Molecular hydrogen reduces mean and systolic blood pressure in various forms of hypertension, as well as inflammatory processes in lung tissue, in Wistar rats DOI
Marina S Artemieva, Т. А. Куропаткина, В. В. Шишкина

et al.

Российский физиологический журнал им  И  М  Сеченова, Journal Year: 2024, Volume and Issue: 110(10), P. 1666 - 1682

Published: Dec. 29, 2024

Molecular hydrogen demonstrates antioxidant and anti-inflammatory properties. It has been shown to have a protective effect in several cardiovascular diseases. The aim of this work was study the breathing atmospheric air containing 4% on degree development monocrotaline-induced pulmonary hypertension associated lung tissue inflammation, as well severity renovascular Wistar rats. Methods. Monocrotaline-induced (MCT-PH) used model small circle hypertension. Three groups animals were experiment: "Control" – injected with monocrotaline solvent, "MCT-Control" "MCT-H2" MCT once. breathed for 21 days, group hydrogen. Inhalations kept constant until days. On day 21, haemodynamic parameters measured under urethane anesthesia samples fixed subsequent morphological analysis. Renovascular 1R1С (RVH) systemic There two RVH-C rats RVH-H2 During experiment, systolic blood pressure (SBP) renal excretory function assessed. 28, anesthesia. Results. In model, had no symptoms hypertension, but decreased mean (MBP), SBP markers connective remodeling lungs, TGF-β MMP-9, resulted tryptase secretion mast cell counts. RVG MBP, function. Conclusion. Inhalation reduces MBP both models arterial inflammatory process, regulates phenotypic functional status cells inhibits activity profibrotic factors MCT-PH. is likely that central action combined its anti-fibrotic effects.

Language: Английский

Therapeutic Potential of Gasdermin D‐Mediated Myocardial Pyroptosis in Ischaemic Heart Disease: Expanding the Paradigm From Bench to Clinical Insights DOI Creative Commons
Chanon Piamsiri, Chayodom Maneechote, Nipon Chattipakorn

et al.

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(3)

Published: Feb. 1, 2025

ABSTRACT Ischaemic heart disease (IHD) remains a leading cause of global morbidity and mortality. One significant contributor to the pathology IHD is excessive release inflammatory mediators during progression. Pyroptosis form programmed cell death (PCD) triggered by activation inflammasomes caspase 1. The 1 proteolytically cleaves gasdermin D (GSDMD) activated amino acid terminus (GSDMD‐NT), disruption plasma membrane. This cascade events considered canonical pathway pyroptosis. also caused oxidative stress, thereby triggering noncanonical pyroptosis via caspases 4/5/11. Previous studies have provided compelling evidence close relationship between aetiology (e.g., acute myocardial infarction, ischaemia reperfusion injury chronic infarction), as well association with unfavourable clinical outcomes. Several interventions aimed at targeting demonstrated promising therapeutic benefits against IHD‐related pathologies. review provides mechanistic insights into roles in from vitro, vivo perspectives. In‐depth understanding this area could pave way for future development novel strategies IHD.

Language: Английский

Citations

0
Hydrogen alleviates myocardial infarction by impeding apoptosis via ROS-mediated mitochondrial endogenous pathway DOI Creative Commons
Shuang Pan, Bin Wang,

Mengshu Yu

et al.

Free Radical Research, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 13

Published: March 5, 2025

Acute myocardial infarction (AMI) is a deadly cardiovascular disease with no effective solution except for percutaneous coronary intervention and artery bypass grafting. Inflammation apoptosis of the injured myocardium after revascularization seriously affect prognosis. Hydrogen possesses anti-inflammatory, anti-oxidative, anti-apoptotic effects may become new treatment AMI. This study explored specific mechanism by which hydrogen operates during AMI treatment. Thirty Sprague-Dawley rats were randomly divided into three groups: control, (MI), + (MI+H2), each containing 10 rats. The MI rat model was established ligation left anterior descending branch. MI+H2 group received 2% inhalation 3 h/Bid. Myocardial infarct size evaluated using triphenyl tetrazolium chloride staining. Transmission electron microscopy showed reduced mitochondrial damage compared group. JC-1 staining, indicates membrane potential, low red/green fluorescence intensity ratio in to that control group, indicating potential loss. After inhalation, this increased, suggesting partial recovery potential. In addition, ATP content, complex I, III activity significantly decreased improved administration. Western blotting analysis Cyt-c protein levels mitochondria increased cytoplasm Following ROS, 8-OHdG, MDA could represent oxidative stress injury decreased. Besides, expression Cyt-C, Bax, cleaved-caspase-9, cleaved-caspase-3 while Bcl-2, TRX2, SOD2 these proteins Overall, reduces size, improves dysfunction, modulates apoptosis-related substances. Importantly, acute downregulating ROS upregulating antioxidant proteins.

Language: Английский

Citations

0
Dietary addition of magnesium hydride nanoparticles: a breakthrough in combating high-fat diet-induced chronic kidney disease DOI
Hongtao Lu, Wanqiu Chen,

Yajing Ying

et al.

Medical Gas Research, Journal Year: 2025, Volume and Issue: 15(3), P. 374 - 382

Published: April 17, 2025

A substantial body of evidence indicates a positive correlation between dyslipidemia and an elevated risk chronic kidney disease, with renal interstitial fibrosis frequently serving as common pathway in the advanced stages disease progression. Hydrogen has anti-inflammatory antioxidant properties, magnesium hydride nanoparticle is material high hydrogen storage capacity. Magnesium -fortified feed capable releasing gas steadily continuously within digestive tract. 12-week high-fat diet significantly serum urea creatinine levels mice. In contrast, dietary addition demonstrated notable protective effect against pathological conditions. Additionally, was found to reduce thereby improve function. support these findings, vitro study utilizing human cortical proximal tubule epithelial cells (HK-2 cells) exposed palmitic acid under conditions mimicking confirmed renoprotective effects hydride. Furthermore, primary target phosphatase tensin homologue deleted on chromosome 10 molecular mechanisms underlying hydride, specifically its ability inhibit transforming growth factor-beta -Smad family member 2 3 (Smad2/3) axis through downregulating expression 10, were elucidated. overexpression Hes BHLH transcription factor 1 can negate beneficial suggesting that may serve upstream regulatory context conclusion, this functions safe effective source inhibiting activation factor-beta/Smad2/3 protein kinase B/mechanistic rapamycin pathways by increasing 10. This mechanism counteracts progression diet-induced damage.

Language: Английский

Citations

0
Molecular Hydrogen Therapy: Mechanisms, Delivery Methods, Preventive, and Therapeutic Application DOI Creative Commons
Jiayi Jin,

Lijun Yue,

Maoru Du

et al.

MedComm, Journal Year: 2025, Volume and Issue: 6(5)

Published: April 28, 2025

ABSTRACT Molecular hydrogen (H 2 ), recognized as the smallest gas molecule, is capable of permeating cellular membranes and diffusing throughout body. Due to its high bioavailability, H considered a therapeutic for treatment various diseases. The efficacy contingent upon factors such administration method, duration contact with diseased tissue, concentration at targeted sites. can be administered exogenously also produced endogenously within intestinal tract. A comprehensive understanding delivery mechanisms modes action crucial advancing medicine. This review highlights H₂’s action, summarizes methods, explores advancements in treating diseases (e.g., inflammatory bowel disease, ischemia–reperfusion, colorectal cancer). Additionally, applications managing other are discussed. Finally, challenges associated clinical application potential solutions explored. We propose that current faced by effectively addressed through use nanoplatforms; furthermore, interactions between gut microbiota may provide insights into Future research should explore synergistic effects conjunction conventional therapies develop personalized plans achieve precision

Language: Английский

Citations

0
Pyroptosis is a drug target for prevention of adverse cardiac remodeling: The crosstalk between pyroptosis, apoptosis, and autophagy DOI Open Access
N. V. Naryzhnaya, Л. Н. Маслов, С. В. Попов

et al.

Journal of Biomedical Research, Journal Year: 2022, Volume and Issue: 36(6), P. 375 - 375

Published: Jan. 1, 2022

Acute myocardial infarction (AMI) is one of the main reasons cardiovascular disease-related death. The introduction percutaneous coronary intervention to clinical practice dramatically decreased mortality rate in AMI. Adverse cardiac remodeling a serious problem cardiology. An increase effectiveness AMI treatment and prevention adverse difficult achieve without understanding mechanisms reperfusion injury remodeling. Inhibition pyroptosis prevents development postinfarction pressure overload-induced remodeling, mitigates cardiomyopathy induced by diabetes metabolic syndrome. Therefore, it reasonable hypothesize that inhibitors may find role for syndrome-triggered cardiomyopathy. It was demonstrated interacts closely with apoptosis autophagy. Pyroptosis could be inhibited nucleotide-binding oligomerization domain-like receptor pyrin domain 3 inhibitors, caspase-1 microRNA, angiotensin-converting enzyme angiotensin Ⅱ blockers, traditional Chinese herbal medicines.

Language: Английский

Citations

17
The NLRP3 Inflammasome as a Novel Therapeutic Target for Cardiac Fibrosis DOI Creative Commons

Jiwen Fan,

Meng Ren,

Binay Kumar Adhikari

et al.

Journal of Inflammation Research, Journal Year: 2022, Volume and Issue: Volume 15, P. 3847 - 3858

Published: July 1, 2022

Abstract: Cardiac fibrosis often has adverse cardiovascular effects, including heart failure, sudden death, and malignant arrhythmias. However, there is no targeted therapy for cardiac fibrosis. Inflammation known to play a crucial role in the disorder, NLR pyrin domain-containing-3 (NLRP3) inflammasome closely associated with innate immunity. Therefore, further understanding pathophysiological of may provide novel strategies prevention treatment disorder. The aim this review was summarize present knowledge NLRP3 inflammasome-related mechanisms underlying suggest potential that could be used treat condition. Keywords: inflammasome, fibrosis, AIM2, ASC, caspase-1

Language: Английский

Citations

16
Elucidating the Molecular Pathways and Therapeutic Interventions of Gaseous Mediators in the Context of Fibrosis DOI Creative Commons
Aohan Li,

Siyuan Wu,

Qian Li

et al.

Antioxidants, Journal Year: 2024, Volume and Issue: 13(5), P. 515 - 515

Published: April 25, 2024

Fibrosis, a pathological alteration of the repair response, involves continuous organ damage, scar formation, and eventual functional failure in various chronic inflammatory disorders. Unfortunately, clinical practice offers limited treatment strategies, leading to high mortality rates diseases. As part investigations into gaseous mediators, or gasotransmitters, including nitric oxide (NO), carbon monoxide (CO), hydrogen sulfide (H2S), numerous studies have confirmed their beneficial roles attenuating fibrosis. Their therapeutic mechanisms, which involve inhibiting oxidative stress, inflammation, apoptosis, proliferation, been increasingly elucidated. Additionally, novel gasotransmitters like (H2) sulfur dioxide (SO2) emerged as promising options for fibrosis treatment. In this review, we primarily demonstrate summarize protective effects mediators process fibrosis, with focus on elucidating underlying molecular mechanisms involved combating

Language: Английский

Citations

2
Controlled Release of Hydrogen‐Carrying Perfluorocarbons for Ischemia Myocardium‐Targeting 19F MRI‐Guided Reperfusion Injury Therapy DOI Creative Commons
Chaoqun Nie,

A Rong,

Jing Wang

et al.

Advanced Science, Journal Year: 2023, Volume and Issue: 10(29)

Published: Aug. 18, 2023

Abstract Hydrogen gas is recently proven to have anti‐oxidative and anti‐inflammation effects on ischemia‐reperfusion injury. However, the efficacy of hydrogen therapy limited by efficiency storage, targeted delivery, controlled release. In this study, H 2 ‐PFOB nanoemulsions (NEs) developed with high loading capacity for ischemic myocardium precision therapy. The hydrogen‐carrying NEs determined chromatography microelectrode methods. Positive uptake in influence 19 F‐MR signal are quantitatively visualized using a 9.4T MR imaging system. biological therapeutic examined myocardial injury mouse model. results illustrated that can efficaciously achieve specific infiltration into exhibit excellent antioxidant anti‐inflammatory properties injury, which be dynamically Moreover, burst release induced low‐intensity focused ultrasound (LIFU) irradiation further promotes effect favorable biosafety profile. potential fully unfolded, may hold great future hydrogen‐based applications tailored

Language: Английский

Citations

6
Effects of Molecular Hydrogen in the Pathophysiology and Management of Metabolic and Non-communicable Diseases DOI
Ram B. Singh, Alex Tarnava, Ján Fedačko

et al.

Advances in biochemistry in health and disease, Journal Year: 2024, Volume and Issue: unknown, P. 287 - 317

Published: Jan. 1, 2024

Language: Английский

Citations

1
Molecular Hydrogen Promotes Adipose-derived Stem Cell Myogenic Differentiation via Regulation of Mitochondria DOI
Yuxia Yang, Wenyong Fei, Mingsheng Liu

et al.

Current Stem Cell Research & Therapy, Journal Year: 2022, Volume and Issue: 18(6), P. 864 - 875

Published: Sept. 27, 2022

Acute skeletal muscle injuries are common physical or sports traumas. Cellular therapy has excellent potential for regeneration after injury. Adipose-derived stem cells (ADSCs) a more accessible type of cell. However, it low survival rate and differentiation efficiency in the oxidative stress-rich microenvironment transplantation. Although molecular hydrogen (H2) possesses anti-inflammatory antioxidant biological properties, its utility mitochondrial cell research not been adequately explored.This study aimed to reveal role H2 on adipose-derived cells' myogenic differentiation.The protective effects ADSCs were evaluated by MTT assay, live-dead staining, western blot analysis, immunofluorescence confocal imaging, transmission electron microscopy.An appropriate volume fraction significantly decreased reactive oxygen species (ROS) levels, increased number mitochondria, promoted mitophagy, thus enhancing ADSCs.This reveals application diseases other pathologies related dysfunction.

Language: Английский

Citations

6