Multi-Faceted Mass Spectrometric Investigation of Neuropeptides in <em>Callinectes sapidus</em> DOI
Ashley Phetsanthad, Nhu Q. Vu, Lingjun Li

et al.

Journal of Visualized Experiments, Journal Year: 2022, Volume and Issue: 183

Published: May 31, 2022

Neuropeptides are signaling molecules that regulate almost all physiological and behavioral processes, such as development, reproduction, food intake, response to external stressors. Yet, the biochemical mechanisms full complement of neuropeptides their functional roles remain poorly understood. Characterization these endogenous peptides is hindered by immense diversity within this class molecules. Additionally, bioactive at concentrations 100x - 1000x lower than neurotransmitters prone enzymatic degradation after synaptic release. Mass spectrometry (MS) a highly sensitive analytical tool can identify, quantify, localize analytes without comprehensive priori knowledge. It well-suited for globally profiling aiding in discovery novel peptides. Due low abundance high chemical peptides, several sample preparation methods, MS acquisition parameters, data analysis strategies have been adapted from proteomics techniques allow optimal neuropeptide characterization. Here, methods described isolating complex biological tissues sequence characterization, quantitation, localization using liquid chromatography (LC)-MS matrix-assisted laser desorption/ionization (MALDI)-MS. A protocol preparing database blue crab, Callinectes sapidus, an organism genomic information, included. These workflows be study other classes different species variety instruments.

Language: Английский

Peptidomics DOI
Roland Hellinger, Arnar Sigurdsson, Wenxin Wu

et al.

Nature Reviews Methods Primers, Journal Year: 2023, Volume and Issue: 3(1)

Published: March 30, 2023

Language: Английский

Citations

57
Mass spectrometry imaging for spatially resolved multi-omics molecular mapping DOI Creative Commons
Hua Zhang,

K. Lu,

Malik Ebbini

et al.

npj Imaging, Journal Year: 2024, Volume and Issue: 2(1)

Published: July 17, 2024

Abstract The recent upswing in the integration of spatial multi-omics for conducting multidimensional information measurements is opening a new chapter biological research. Mapping landscape various biomolecules including metabolites, proteins, nucleic acids, etc., and even deciphering their functional interactions pathways believed to provide more holistic nuanced exploration molecular intricacies within living systems. Mass spectrometry imaging (MSI) stands as forefront technique spatially mapping metabolome, lipidome, proteome diverse tissue cell samples. In this review, we offer systematic survey delineating different MSI techniques resolved analysis, elucidating principles, capabilities, limitations. Particularly, focus on advancements methodologies aimed at augmenting sensitivity specificity MSI; depict burgeoning MSI-based metabolomics, lipidomics, proteomics, encompassing synergy with other modalities. Furthermore, speculative insights into potential trajectory technology future.

Language: Английский

Citations

18
Origins, Technological Advancement, and Applications of Peptidomics DOI
Michael Schrader

Methods in molecular biology, Journal Year: 2024, Volume and Issue: unknown, P. 3 - 47

Published: Jan. 1, 2024

Language: Английский

Citations

4
Pigment-dispersing factor neuropeptides act as multifunctional hormones and modulators in tardigrades DOI Creative Commons
Soumi Dutta, Lars Hering, Milena M. Grollmann

et al.

Open Biology, Journal Year: 2025, Volume and Issue: 15(3)

Published: March 1, 2025

Pigment-dispersing factors (PDFs) are neuropeptides that play key roles in controlling the circadian rhythms various insects, whereas their function remains elusive other protostomes including tardigrades (water bears). Here we show three PDFs of tardigrade Hypsibius exemplaris co-localized two pairs inner lobe cells brain, only one PDF occurs four additional cerebral and extracerebral cells. The axons pass through contralateral brain hemisphere, descend to ventral nerve cord terminate potential release sites posteriormost trunk ganglion. Using vitro assays, demonstrate all deorphanized receptor (PDFR) functional. Widespread localization PDFR suggests may act as multifunctional hormones neuromodulators control major functions light detection, neural processing, locomotion, feeding, digestion, osmoregulation, growth, embryonic development oogenesis/reproduction.

Language: Английский

Citations

0
Multisensory navigation and neuronal plasticity in desert ants DOI Creative Commons
Wolfgang Rößler

Trends in Neurosciences, Journal Year: 2023, Volume and Issue: 46(6), P. 415 - 417

Published: April 4, 2023

Cataglyphis desert ants are skilled visual navigators. Here, I present a brief overview of multisensory learning and neuronal plasticity in ants, with particular focus on the transition from dark nest interior to performing first foraging trips. This highlights as experimental models for studying mechanisms underlying behavioral development into successful

Language: Английский

Citations

8
Unravel the Local Complexity of Biological Environments by MALDI Mass Spectrometry Imaging DOI Open Access
Elvira Sgobba, Yohann Daguerre, Marco Giampà

et al.

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(22), P. 12393 - 12393

Published: Nov. 17, 2021

Classic metabolomic methods have proven to be very useful study functional biology and variation in the chemical composition of different tissues. However, they do not provide any information terms spatial localization within fine structures. Matrix-assisted laser desorption ionization mass spectrometry imaging (MALDI MSI) does reaches at best a resolution 0.25 μm depending on setup, making it powerful tool analyze local complexity biological samples cellular level. Here, we intend give an overview diversity molecules localizations analyzed using this method as well update latest adaptations made circumvent samples. MALDI MSI has been widely used medical sciences is now developing research areas diverse entomology, microbiology, plant biology, plant–microbe interactions, rhizobia symbiosis being most exhaustively described so far. Those are fields interest which will focus demonstrate strengths characterizing distributions metabolites, lipids, peptides relation questions.

Language: Английский

Citations

20
Integrated mass spectrometry imaging and metabolomics reveals sublethal effects of indoxacarb on the red fire ant Solenopsis invicta DOI

Mingyi Du,

Zhibin Yin,

Kaijie Xu

et al.

Pest Management Science, Journal Year: 2023, Volume and Issue: 79(9), P. 3122 - 3132

Published: April 4, 2023

Abstract BACKGROUND Indoxacarb, representing an efficient insecticide, is normally made into a bait to spread the poison among red fire ants so that it can be widely applied in prevention and control of Solenopsis invicta . However, potential toxicity mechanism S. response indoxacarb remains explored. In this study, we integrated mass spectrometry imaging (MSI) untargeted metabolomics methods reveal disturbed metabolic expression levels spatial distribution within whole‐body tissue treated with indoxacarb. RESULTS Metabolomics results showed significantly altered level metabolites after treatment, such as carbohydrates, amino acids pyrimidine derivatives. Additionally, regulation several crucial resulting from pathway lipids visualized using label‐free MSI methods. Specifically, xylitol, aspartate, uracil were distributed throughout whole body , while sucrose‐6′‐phosphate glycerol mainly abdomen thymine was head chest Taken together, indicated closely associated disturbance key pathways, metabolism, aspartate pentose glucuronate interconversions, inhibited energy synthesis. CONCLUSION Collectively, these findings provide new perspective for understanding assessment between targeted organisms pesticides. © 2023 Society Chemical Industry.

Language: Английский

Citations

7
Differential expression of some termite neuropeptides and insulin/IGF-related hormones and their plausible functions in growth, reproduction and caste determination DOI Creative Commons
Jan A. Veenstra

PeerJ, Journal Year: 2023, Volume and Issue: 11, P. e15259 - e15259

Published: April 26, 2023

Background Insulin-like growth factor (IGF) and other insulin-like peptides (ilps) are important hormones regulating development in animals. Whereas most animals have a single female male adult phenotype, some insect species the same genome may lead to different final forms. Perhaps best known example is honeybee where females can either develop into queens or workers. More extreme forms of such polyphenism occur termites, queens, kings, workers soldiers coexist. Both juvenile hormone regulate reproduction as well polyphenism. In termites role induction soldier caste known, but IGF ilps these processes remains largely unknown. Here various termite identified hypotheses regarding their functions suggested. Methods Genome assemblies transcriptome short read archives (SRAs) were used identify neuropeptides determine expression species, tissues castes. Results Discussion Termites seven ilps, i.e. gonadulin, an ortholog Drosophila peptide 7 (dilp7), which commonly present insects, four smaller peptides, that collectively been called IGF-related (sirps) individually atirpin, birpin, cirpin brovirpin. Gonadulin lost from higher however amplified brovirpin gene, they often two three paralogs. Based on differential genes it seems likely atirpin autocrine tissue released when faces metabolic stress. Birpin be responsible for absence this reproductive adults or, present, soldiers. Brovirpin expressed both by brain ovary stimulates vitellogenesis, while function less clear.

Language: Английский

Citations

7
Neuropeptide regulations on behavioral plasticity in social insects DOI
Li Hou,

N. Wang,

Tianle Sun

et al.

Current Opinion in Insect Science, Journal Year: 2023, Volume and Issue: 60, P. 101119 - 101119

Published: Sept. 22, 2023

Language: Английский

Citations

6
Neuropeptides as potential modulators of behavioral transitions in the ant Cataglyphis nodus DOI
Jens Habenstein, Markus Thamm, Wolfgang Rößler

et al.

The Journal of Comparative Neurology, Journal Year: 2021, Volume and Issue: 529(12), P. 3155 - 3170

Published: May 6, 2021

Abstract Age‐related behavioral plasticity is a major prerequisite for the ecological success of insect societies. Although aspects flexibility have been targeted in many studies, underlying intrinsic mechanisms controlling diverse changes behavior along individual life history social insects are not completely understood. Recently, neuropeptides allatostatin‐A, corazonin, and tachykinin associated with regulation transitions insects. Here, we investigated brain localization expression these following Cataglyphis nodus ants. Our immunohistochemical analyses revealed that overall branching pattern neurons immunoreactive (ir) three largely independent stages. Numerous allatostatin‐A‐ tachykinin‐ir innervate primary sensory neuropils high‐order integration centers brain. In contrast, number corazonergic restricted to only four per hemisphere cell bodies located pars lateralis axons extending medial protocerebrum retrocerebral complex. Most interestingly, cell‐body volumes significantly increased foragers compared freshly eclosed ants interior workers. Quantification mRNA levels stage‐related change allatostatin‐A corazonin Given presence control neurohemal organs, mRNA‐changes strongly suggest an important modulatory role both maturation

Language: Английский

Citations

14