Smads and AP‐1 activation of TGF‐β signaling upregulate transcription of Osteoprotegerin in Cementoblasts to inhibit osteoclastogenesis DOI Open Access
L. H. Mo, Jiaqi Zhu, M. Li

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(22)

Published: Nov. 15, 2024

Abstract Orthodontically induced root resorption (OIRR) is a common side effect during orthodontic tooth treatment (OTM). The function of osteoprotegerin (OPG) considered to protect cementum from excessive maintain integrity. In this study, we observed that the expression TGF‐β1 and OPG was upregulated under loading force in periodontal tissues. However, specific molecular mechanisms TGF‐β1‐induced cementoblasts are not fully elucidated. This study aims investigate Smads AP‐1 stimulated by TGF‐β signaling on transcription cementoblasts. vitro, demonstrated TGF‐β/Smad involved extracellular secretion conditioned media (CM) cementoblasts, which further inhibited osteoclastogenesis. Reporter gene plasmids containing promoter sequences different lengths (0.5–3 kb) were constructed potential binding sites AP‐1. We identified nine concentrated 0–0.5 2.3–3 kb regions ChlP results showed Smad2/3/4 c‐Jun bound more stimulation. vivo, localized administration OTM model increased expression, resulted inhibition OIRR. summary, can bind promote transcription, inhibits osteoclast differentiation protects resorption.

Language: Английский

Matrix Metalloproteinases in the Periodontium—Vital in Tissue Turnover and Unfortunate in Periodontitis DOI Open Access
Dominik Radzki, Alessandro Negri, Aida Kusiak

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2763 - 2763

Published: Feb. 27, 2024

The extracellular matrix (ECM) is a complex non-cellular three-dimensional macromolecular network present within all tissues and organs, forming the foundation on which cells sit, composed of proteins (such as collagen), glycosaminoglycans, proteoglycans, minerals, water. ECM provides fundamental framework for cellular constituents tissue biochemical support to surrounding cells. highly dynamic structure that constantly being remodeled. Matrix metalloproteinases (MMPs) are among most important proteolytic enzymes capable degrading molecules. MMPs play relevant role in physiological well pathological processes; participate embryogenesis, morphogenesis, wound healing, remodeling, therefore, their impaired activity may result several problems. MMP also associated with chronic inflammation, breakdown, fibrosis, cancer invasion metastasis. periodontium unique anatomical site, variety connective tissues, created by ECM. During periodontitis, inflammation affecting periodontium, increased presence observed, resulting irreversible losses periodontal tissues. expression be controlled various ways, one inhibition an endogenous group inhibitors (TIMPs), reversion-inducing cysteine-rich protein Kazal motifs (RECK).

Language: Английский

Citations

12
Effects of mechanical loading on matrix homeostasis and differentiation potential of periodontal ligament cells: A scoping review DOI

Novena Dameria Pakpahan,

Maythwe Kyawsoewin, Jeeranan Manokawinchoke

et al.

Journal of Periodontal Research, Journal Year: 2024, Volume and Issue: 59(5), P. 877 - 906

Published: May 12, 2024

Various mechanical loadings, including stress, orthodontics forces, and masticatory force, affect the functions of periodontal ligament cells. Regulation tissue destruction, formation, differentiation are crucial processes for regeneration therapy. Numerous studies have reported that different types loading play a role in maintaining matrix homeostasis, osteogenic This scoping review aims to evaluate regarding effects various loadings on secretion extracellular (ECM) components, regulation balance between formation destruction matrix, differentiation, multiple ligament. An electronic search this has been conducted two databases; MEDLINE via PubMed SCOPUS. Study selection criteria included original research written English homeostasis potential The final 204 articles were mainly present review. Mechanical forces appropriate magnitude, duration, pattern positive influence ECM components such as collagen, well regulate metalloproteinases inhibitors metalloproteinases. Additionally, these osteoblastic osteoclast differentiation. Conversely, incorrect can lead abnormal both soft hard tissue. provides additional insight into how impact cells (PDLCs), thus making it valuable regenerative treatment. In combination with advancing technologies, utilization application aspects PDLCs could bring benefits future

Language: Английский

Citations

8
Smads and AP‐1 activation of TGF‐β signaling upregulate transcription of Osteoprotegerin in Cementoblasts to inhibit osteoclastogenesis DOI Open Access
L. H. Mo, Jiaqi Zhu, M. Li

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(22)

Published: Nov. 15, 2024

Abstract Orthodontically induced root resorption (OIRR) is a common side effect during orthodontic tooth treatment (OTM). The function of osteoprotegerin (OPG) considered to protect cementum from excessive maintain integrity. In this study, we observed that the expression TGF‐β1 and OPG was upregulated under loading force in periodontal tissues. However, specific molecular mechanisms TGF‐β1‐induced cementoblasts are not fully elucidated. This study aims investigate Smads AP‐1 stimulated by TGF‐β signaling on transcription cementoblasts. vitro, demonstrated TGF‐β/Smad involved extracellular secretion conditioned media (CM) cementoblasts, which further inhibited osteoclastogenesis. Reporter gene plasmids containing promoter sequences different lengths (0.5–3 kb) were constructed potential binding sites AP‐1. We identified nine concentrated 0–0.5 2.3–3 kb regions ChlP results showed Smad2/3/4 c‐Jun bound more stimulation. vivo, localized administration OTM model increased expression, resulted inhibition OIRR. summary, can bind promote transcription, inhibits osteoclast differentiation protects resorption.

Language: Английский

Citations

1