High temperatures promote antibiotic resistance genes conjugative transfer under residual chlorine: Mechanisms and risks

Journal of Hazardous Materials, Journal Year: 2024, Volume and Issue: 483, P. 136675 - 136675

Published: Nov. 26, 2024

Language: Английский

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Of telomeres and temperature: Measuring thermal effects on telomeres in ectothermic animals

Molecular Ecology, Journal Year: 2021, Volume and Issue: 31(23), P. 6069 - 6086

Published: Aug. 27, 2021

Ectotherms are classic models for understanding life-history tradeoffs, including the reproduction-somatic maintenance tradeoffs that may be reflected in telomere length and their dynamics. Importantly, traits of ectotherms tightly linked to thermal environment, with diverse or synergistic mechanistic explanations underpinning variation. Telomere dynamics potentially provide a link can used monitor effects on individuals response climatic perturbations. Growth rate, age developmental stage all affected by temperature, which interacts complex intriguing ways. The physiological processes visualized understood using performance curves (TPCs). TPCs reflect evolutionary history environment during an individual's ontogeny. should enhanced at near optimum species, population individual. sensitivity interacting underlying them. key directly mitochondrial function (reactive oxygen production), antioxidant activity, telomerase activity endcap protein status. We argue identifying these will significantly help design robust, repeatable experiments field studies ectotherms. Conceptually, valuable framework predict interpret taxon- population-specific across regimes. literature telomeres is sparse mostly limited vertebrates, but our conclusions recommendations relevant ectothermic animals.

Language: Английский

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Mono-(2-ethylhexyl) Phthalate (MEHP)-Induced Telomere Structure and Function Disorder Mediates Cell Cycle Dysregulation and Apoptosis via c-Myc and Its Upstream Transcription Factors in a Mouse Spermatogonia-Derived (GC-1) Cell Line

Toxics, Journal Year: 2023, Volume and Issue: 11(5), P. 448 - 448

Published: May 10, 2023

As a typical environmental endocrine disrupting chemical (EDC), di-(2-ethylhexyl) phthalate (DEHP) is thought to be related reproductive disorders, especially in males. Growing evidence suggests that various EDCs may result an impaired telomere structure and function, which associated with male infertility. However, the adverse effect of DEHP on telomeres cells has rarely been studied, mechanisms remain unclear. In this study, we tested effects mono-(2-ethylhexyl) (MEHP), primary metabolite DEHP, dysfunction mouse spermatogonia-derived (GC-1) potential role TERT c-Myc MEHP-induced spermatogenic cell damage. Results showed MEHP induced viability inhibition, G0/G1 phase cycle arrest, apoptosis GC-1 dose-dependent manner. Shortened telomeres, reduced telomerase activity, decreased expression TERT, c-Myc, upstream transcription factors were also observed MEHP-treated cells. conclusion, it can concluded TERT-mediated contribute arrest through impairment its factors.

Language: Английский

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Mitochondrial dysfunction and oxidative stress in Alzheimer's disease–A step towards mitochondria based therapeutic strategies

Aging and Health Research, Journal Year: 2023, Volume and Issue: 3(4), P. 100169 - 100169

Published: Nov. 8, 2023

Alzheimer's disease (AD) is a neurodegenerative with reduced cognitive function due to mitochondrial dysfunction and oxidative stress. Recent studies show that the pathophysiology of AD may be influenced by dysfunctionality, Ca2+ imbalance, apoptosis, decreased energy, alteration in its metabolism. Study indicates damaged mitochondria play critical roles pathogenesis AD, even if precise mechanism behind remains unknown. It thought healthy pool protects neurons reducing damage caused also promotes neuronal activity giving enough energy other associated functions. In this sense, investigation mechanisms altered constitutes novel, promising therapeutic targets for disease. Mitochondria enhances generation, antioxidants scavenge reactive oxygen species reduce substrate supply, glucose metabolism, potential drug candidates target apoptotic mitophagy pathways remove mitochondria. Although therapy approaches have shown promise preclinical studies, there hasn't been much advancement clinical trials thus far. Therefore, we try find out role highlight development compounds as AD.

Language: Английский

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Comparative morphological, physiological, and biochemical traits in sensitive and tolerant maize genotypes in response to salinity and pb stress

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 28, 2024

Salinity and lead are two important abiotic stresses that limit crop growth yield. In this study, we assayed the effect of these on tolerant sensitive maize genotypes. Four-week-old plants were treated with 250 mM sodium chloride (NaCl) µM (Pb). Our results show NaCl or Pb treatment genotype caused a significant reduction in root length, plant height, total fresh dry weights, as well chlorophyll, carotenoid content. Salt stress led to decrease relative water content, shoot weight leaf area K+ content but increase Na+ Both increased antioxidant enzyme activity, proline malondialdehyde, hydrogen peroxide levels. Principal component analysis (PCA) accounted for 69.8% 16.5% variation among all variables studied. PCA also suggested positive correlation between peroxide, peroxidase, catalase, ascorbate peroxidase levels, negative chlorophyll area, fresh, weights. Together, suggest salt-tolerant is more suitable adapting compared salt-sensitive genotype.

Language: Английский

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The antibacterial mechanism of phenylacetic acid isolated from Bacillus megaterium L2 against Agrobacterium tumefaciens

PeerJ, Journal Year: 2022, Volume and Issue: 10, P. e14304 - e14304

Published: Nov. 8, 2022

Background Agrobacterium tumefaciens T-37 can infect grapes and other fruit trees cause root cancer. Given the pollution damage of chemical agents to environment, use biological control has become an important area focus. Bacillus megaterium L2 is a beneficial biocontrol strain isolated identified in laboratory, which good antibacterial effect on variety plant pathogens. The metabolites were separated purified obtain bioactive compound phenylacetic acid (PAA). Methods potential mechanism PAA against A. was determined by relative conductivity, leakage nucleic acids, proteins, soluble total sugars, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), reactive oxygen species (ROS). Results showed activity with IC 50 0.8038 mg/mL. Our data suggested that after treatment PAA, acid, protein, sugar increased significantly compared chloramphenicol group negative group. protein synthesis cells inhibited, consumption phosphorus decreased increase incubation time, content ROS higher than Meanwhile, two key enzymes (MDH SDH) involved tricarboxylic cycle (TCA cycle) decreased. In addition, found be damaged scanning electron microscopy observation. results destroy cell membrane integrity, structures, affect metabolism, inhibit exert effect. Conclusions We concluded action might described as exerting affecting inhibiting synthesis, destroying integrity ultrastructure. Therefore, promising application prospect prevention cancer disease caused .

Language: Английский

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Telomere dynamics in maturing frogs vary among organs

Biology Letters, Journal Year: 2025, Volume and Issue: 21(2)

Published: Feb. 1, 2025

It is important to know whether organs age at the same rate and are equally affected by developmental conditions as this provides insights into causes of ageing. However, data on organ-specific telomere dynamics remain scant. In a previous study early life amphibian Xenopus laevis , we detected changes in lengths gut cells, while liver, heart muscle telomeres were unchanged; larval rearing temperature had minimal effects. Here, extend that examine four treatments from 70-day post-metamorphic juvenile through sexually mature (2-year-old) adults. Telomeres shortened adult gut, hindlimb muscle. contrast, liver did not change with but shorter if was warm. Organ influenced sex only Warmer temperatures also associated longer juveniles. Hence, pre-metamorphic can influence dynamics, loss between stages occurs different those earlier life. These findings indicate existence organ-dependent ageing rates across lifetimes, potentially related environmental history.

Language: Английский

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Unraveling the cGAS-STING pathway in Alzheimer’s disease: A new Frontier in neuroinflammation and therapeutic strategies

Neuroscience, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

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Aging Processes Are Affected by Energy Balance: Focused on the Effects of Nutrition and Physical Activity on Telomere Length

Current Nutrition Reports, Journal Year: 2024, Volume and Issue: 13(2), P. 264 - 279

Published: March 18, 2024

The number and proportion of individuals aged 60 over are increasing globally. increase in the elderly population has important social economic effects. Telomere length is an marker for healthy aging. Here, we review relevance between telomere energy balance by determining effects physical activity, nutrients, dietary patterns, foods on aging with related studies. Evidence emphasizes importance integrity It also focuses potential interventions such as activity a diet to improve this process. We suggest that ensuring regular diets can contribute process protecting length. In addition, different methods studies, short inconsistent durations, types exercise, non-standard have led conflicting results. More studies needed elucidate molecular-based mechanisms.

Language: Английский

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Genomic approaches to mitigating genetic diversity loss in declining populations

Molecular Ecology, Journal Year: 2023, Volume and Issue: 32(19), P. 5228 - 5240

Published: Aug. 23, 2023

Abstract The accelerating pace of global biodiversity loss is exacerbated by habitat fragmentation and subsequent inbreeding in small populations. To address this problem, conservation practitioners often turn to assisted breeding programmes with the aim enhancing genetic diversity declining Although genomic information infrequently included these efforts, it has potential significantly enhance success such programmes. In study, we showcase value approaches for increasing specifically focusing on a highly inbred population Western burrowing owls. maximize resulting offspring, begin creating an optimal pairing decision tree based sex, kinship patterns homozygosity across genome. evaluate effectiveness our strategy, compare diversity, brood size nestling rates between optimized non‐optimized pairs. Additionally, leverage recently discovered correlations telomere length fitness species investigate whether optimization could have long‐term benefits. Our results indicate that individuals contrasting genome effective way increase offspring. short‐term field‐based metrics did not differ pairs, offspring from pairs had longer telomeres, suggesting can help reduce risk depression. These findings underscore importance tools informing efforts preserve adaptive small, populations at further decline.

Language: Английский

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