The mycobiome in human cancer: analytical challenges, molecular mechanisms, and therapeutic implications

Molecular Cancer, Journal Year: 2025, Volume and Issue: 24(1)

Published: Jan. 15, 2025

The polymorphic microbiome is considered a new hallmark of cancer. Advances in High-Throughput Sequencing have fostered rapid developments research. interaction between cancer cells, immune and microbiota defined as the immuno-oncology (IOM) axis. Fungal microbes (the mycobiome), although representing only ∼ 0.1-1% microbiome, are critical immunologically active component tumor microbiome. Accumulating evidence suggests possible involvement commensal pathogenic fungi initiation, progression, treatment responsiveness. tumor-associated mycobiome mainly consists gut mycobiome, oral intratumoral mycobiome. However, role remains poorly understood, diversity complexity analytical methods make it challenging to access this field. This review aims elucidate causal complicit roles development progression while highlighting issues that need be addressed executing such We systematically summarize advantages limitations current fungal detection analysis methods. enumerate integrate these recent findings into our understanding accompanied by prospect novel exhilarating clinical implications.

Language: Английский

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Fungi and cancer

Gut, Journal Year: 2023, Volume and Issue: 72(7), P. 1410 - 1425

Published: May 5, 2023

The microbiome may impact cancer development, progression and treatment responsiveness, but its fungal components remain insufficiently studied in this context. In review, we highlight accumulating evidence suggesting a possible involvement of commensal pathogenic fungi modulation cancer-related processes. We discuss the mechanisms by which can influence tumour biology, locally activity exerted within microenvironment, or remotely through secretion bioactive metabolites, host immunity communications with neighbouring bacterial commensals. examine prospects utilising fungi-related molecular signatures diagnosis, patient stratification assessment while highlighting challenges limitations faced performing such research. all, demonstrate that likely constitute important members mucosal tumour-residing microbiomes. Exploration inter-kingdom interactions decoding their causal impacts on biology enable harnessing into diagnosis treatment.

Language: Английский

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A comprehensive analysis of the effect of quorum-sensing molecule 3-oxo-C12-homoserine lactone on Candida auris and Candida albicans

Biofilm, Journal Year: 2025, Volume and Issue: unknown, P. 100259 - 100259

Published: Jan. 1, 2025

Language: Английский

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Unveiling the hidden players: exploring the role of gut mycobiome in cancer development and treatment dynamics

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: March 14, 2024

The role of gut fungal species in tumor-related processes remains largely unexplored, with most studies still focusing on infections. This review examines the accumulating evidence suggesting involvement commensal and pathogenic fungi cancer biological process, including oncogenesis, progression, treatment response. Mechanisms explored include influence host immunity, secretion bioactive toxins/metabolites, interaction bacterial commensals, migration to other tissues certain types cancers. Attempts utilize molecular signatures for diagnosis fungal-derived products are discussed. A few highlight fungi's impact responsiveness sensitivity chemotherapy, radiotherapy, immunotherapy, fecal microbiota transplant. Given limited understanding techniques research, facing great challenges, despite having potentials.

Language: Английский

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Roles of Pseudomonas aeruginosa siderophores in interaction with prokaryotic and eukaryotic organisms

Research in Microbiology, Journal Year: 2024, Volume and Issue: 175(7), P. 104211 - 104211

Published: May 9, 2024

Language: Английский

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A gain-of-function mutation in zinc cluster transcription factor Rob1 drives Candida albicans adaptive growth in the cystic fibrosis lung environment

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(4), P. e1012154 - e1012154

Published: April 11, 2024

Candida albicans chronically colonizes the respiratory tract of patients with Cystic Fibrosis (CF). It competes CF-associated pathogens (e.g. Pseudomonas aeruginosa) and contributes to disease severity. We hypothesize that C. undergoes specific adaptation mechanisms explain its persistence in CF lung environment. To identify underlying genetic phenotypic determinants, we serially recovered 146 clinical isolates over a period 30 months from sputum 25 antifungal-naive patients. Multilocus sequence typing analyses revealed most were individually colonized genetically close strains, facilitating comparative between serial isolates. strikingly observed differential ability filament form monospecies dual-species biofilms P. aeruginosa among 18 sharing same diploid type, within one year pediatric patient. Whole genome sequencing their genomes highly heterozygous similar each other, displaying clonal subpopulation structure. Data mining identified 34 non-synonymous SNPs 19 open reading frames differentiating hyperfilamentous strong biofilm-former strains remaining Among these, detected glycine-to-glutamate substitution at position 299 (G299E) deduced amino acid zinc cluster transcription factor ROB1 (ROB1G299E), encoding major regulator filamentous growth biofilm formation. Introduction G299E mutation co-isolated weak strain was sufficient confer growth, increased expression hyphal-specific genes, formation survival formed aeruginosa, indicating ROB1G299E is gain-of-function mutation. Disruption isolate carrying allele abolished hyperfilamentation Our study links single better survive during interaction other microbes illuminates how adaptive traits emerge microbial persistently colonize and/or infect CF-patient airways.

Language: Английский

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Synergistic Antimicrobial Activity of Silver Nanoparticles with an Emergent Class of Azoimidazoles

Pharmaceutics, Journal Year: 2023, Volume and Issue: 15(3), P. 926 - 926

Published: March 13, 2023

The combination of two or more agents capable acting in synergy has been reported as a valuable tool to fight against pathogens. Silver nanoparticles (AgNPs) present strong antimicrobial action, although their cytotoxicity for healthy cells at active concentrations is major concern. Azoimidazole moieties exhibit interesting bioactivities, including activity. In this work, class recently described azoimidazoles with antifungal activity was conjugated citrate polyvinylpyrrolidone-stabilized AgNPs. Proton nuclear magnetic resonance used confirm the purity compounds before further tests and atomic absorption spectroscopy verify concentration silver prepared dispersions. Other analytical techniques elucidate morphology stability AgNPs corresponding conjugates, namely ultraviolet-visible spectrophotometry, scanning transmission electron microscopy dynamic light scattering analysis. synergistic conjugates assessed through checkerboard assay yeasts (Candida albicans Candida krusei) bacteria (Staphylococcus aureus Escherichia coli). showed improved all microorganisms, particular towards bacteria, below individual minimal inhibitory (MIC). Furthermore, some combinations were found be non-cytotoxic human HaCaT cells.

Language: Английский

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Herptile gut microbiomes: a natural system to study multi-kingdom interactions between filamentous fungi and bacteria

mSphere, Journal Year: 2024, Volume and Issue: 9(3)

Published: Feb. 13, 2024

ABSTRACT Reptiles and amphibians (herptiles) are some of the most endangered threatened species on planet numerous conservation strategies being implemented with goal ensuring recovery. Little is known, however, about gut microbiome wild herptiles how it relates to health these populations. Here, we report results from characterization both a broad survey herptiles, correlation between fungus Basidiobolus , bacterial community supported by deeper, more intensive sampling Plethodon glutinosus known as slimy salamanders. We demonstrate that communities sampled frogs, lizards, salamanders structured host taxonomy common natural component microbiomes. Intensive multiple hosts across ecoregions Tennessee revealed geography host:geography interactions strong predictors distinct operational taxonomic units present within given host. Co-occurrence analyses diversity support interaction bacteria, suggesting may play role in structuring community. further hypothesis this advanced unique specialized metabolism originating horizontal gene transfer bacteria capable producing metabolites including small cyclic peptides. IMPORTANCE This work significantly advances our understanding biodiversity microbial herptile microbiomes, fungi structural functional members chemical functions structure phenotypes. also provide an important observational system represents environment selects for novel metabolic through bacteria. Such studies needed better understand complexity microbiomes nature will inform herpetofauna.

Language: Английский

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Evaluation of the resistome and gut microbiome composition of hospitalized patients in a health unit of southern Brazil coming from a high animal husbandry production region

Frontiers in Antibiotics, Journal Year: 2025, Volume and Issue: 3

Published: Jan. 17, 2025

Antimicrobial resistance (AMR) poses a significant threat to global public health. The One Health approach, which integrates human, animal, and environmental health, highlights the roles of agricultural hospital settings in propagation AMR. This study aimed analyze resistome gut microbiome composition individuals from high-intensity animal husbandry area western region Santa Catarina, Southern Brazil, who were subsequently admitted University Hospital city Florianopolis, located eastern part same state. Rectal swab samples collected upon admission discharge. Metagenomic sequencing analysis employed identify antimicrobial genes (ARGs) their associated bacterial taxa. Additionally, impact environment on profiles these patients was assessed. A total 247 genetic elements related AMR identified, with 66.4% present both discharge samples. Aminoglycoside most prevalent, followed by for tetracyclines lincosamides. Notably, unique genes, including dfrF mutations gyrB, identified at ARGs 55 species, Lactobacillus fermentum, harboring ermB gene. (MLSB), detected prevalent families included Mycobacteriaceae, Enterobacteriaceae, Bacteroidaceae. Among these, Mycobacteriaceae abundant, primarily 16S rRNA gene, RNA polymerase subunits, gyrases. revealed high prevalence aminoglycoside tetracycline resistance, notable increase certain determinants discharge, likely influenced extended use. presence mcr colistin single patient concerning trend AMR, particularly relation husbandry. These findings underscore substantial use development complex dynamics settings. They also emphasize influence local factors, such as intensive production, patterns advocate ongoing surveillance policy manage multidrug-resistant bacteria eVectively.

Language: Английский

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Predicting the risk of invasive fungal infections in ICU sepsis population: the AMI risk assessment tool

Infection, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 3, 2025

Language: Английский

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