The effects of glyphosate, pure or in herbicide formulation, on bumble bees and their gut microbial communities

The Science of The Total Environment, Journal Year: 2023, Volume and Issue: 872, P. 162102 - 162102

Published: Feb. 9, 2023

Language: Английский

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Bees just wanna have fungi: a review of bee associations with nonpathogenic fungi

FEMS Microbiology Ecology, Journal Year: 2023, Volume and Issue: 99(8)

Published: July 7, 2023

Abstract Bee–fungus associations are common, and while most studies focus on entomopathogens, emerging evidence suggests that bees associate with a variety of symbiotic fungi can influence bee behavior health. Here, we review nonpathogenic fungal taxa associated different species bee-related habitats. We synthesize results examining effects behavior, development, survival, fitness. find communities differ across habitats, some groups restricted mostly to flowers (Metschnikowia), others present almost exclusively in stored provisions (Zygosaccharomyces). Starmerella yeasts found multiple habitats association many species. Bee widely the abundance identity hosted. Functional suggest affect foraging, pathogen interactions, though few have been examined this context. Rarely, obligately beneficial symbionts bees, whereas facultative associates unknown or ecologically contextual effects. Fungicides reduce alter potentially disrupting bee–fungi associations. recommend future study non-honeybee examine life stages document composition, abundance, mechanistic bees.

Language: Английский

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Exploring climate‐related gut microbiome variation in bumble bees: An experimental and observational perspective

Ecology, Journal Year: 2025, Volume and Issue: 106(3)

Published: March 1, 2025

Abstract Rising temperatures negatively affect bumble bee fitness directly through physiological impacts and indirectly by disrupting mutualistic interactions between bees other organisms, which are crucial in determining species‐specific responses to climate change. Gut microbial symbionts, key regulators of host nutrition health, may be the Achilles' heel thermal insects. They not only modulate biotic with plants pathogens but also exhibit varying sensitivity themselves. Understanding how environmental changes disrupt microbiome communities is a first step determine potential consequences for population responses. We analyzed gut bacterial six species inhabiting different climatic niches along an elevational gradient German Alps using 16S ribosomal DNA amplicon sequencing. investigated whether inter‐ intraspecific differences can linked species' niches, differ temperature, flower resource composition, likely pathogen pressure. A reciprocal translocation experiment distinct regions tested Bombus terrestris lucorum change short‐term when exposed new environments. Finally, we these heat cold wave scenarios within chambers disentangle pure temperature‐driven effects on from effects. Interspecific variation composition exceeded variation. Species levels stability, where stability defined as within‐group variance: lower indicated greater variance, predominantly observed higher elevations. Transplanted showed subtle adjustments, marked increase Lactobacillaceae upon exposure warmer regions; however, microbiomes did under laboratory temperature scenarios. conclude that could lead For example, less stable elevations might indicate increased pathogens. Short‐term following relatively microbiomes, such B. , rapidly integrate bacteria, their capacity cope environments

Language: Английский

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Microbiome assembly and maintenance across the lifespan of bumble bee workers

Molecular Ecology, Journal Year: 2022, Volume and Issue: 32(3), P. 724 - 740

Published: Nov. 5, 2022

Abstract How a host's microbiome changes over its lifespan can influence development and ageing. As these temporal patterns have only been described in detail for handful of hosts, an important next step is to compare succession more broadly investigate why it varies. Here we characterize the dynamics stability bumble bee worker gut microbiome. Bumble bees simple host‐specific microbiomes, their microbial may health pollination services. We used 16S rRNA gene sequencing, quantitative PCR metagenomics microbiomes Bombus impatiens workers. also sequenced transcriptomes examine host factors that control At community level, assembly highly predictable similar primary observed human gut. However, at strain partitioning bacterial variants among colonies suggests stochastic colonization events those flies nematodes. find strong differences symbiont species, suggesting ecological members persistence. Finally, show both transcriptome—including expression key immunity genes—stabilize, as opposed senesce, with age. suggest social groups such bees, maintenance contribute inclusive fitness, thus remain under selection even old Our findings provide foundation exploring mechanisms functional outcomes succession.

Language: Английский

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Bumble bee microbiota shows temporal succession and increase of lactic acid bacteria when exposed to outdoor environments

Frontiers in Cellular and Infection Microbiology, Journal Year: 2024, Volume and Issue: 14

Published: March 4, 2024

The large earth bumble bee (

Language: Английский

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Pollen Diet Diversity does not Affect Gut Bacterial Communities or Melanization in a Social and Solitary Bee Species

Microbial Ecology, Journal Year: 2024, Volume and Issue: 87(1)

Published: Jan. 2, 2024

Language: Английский

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Conserved, yet disruption-prone, gut microbiomes in neotropical bumblebees

mSphere, Journal Year: 2023, Volume and Issue: 8(6)

Published: Oct. 19, 2023

Social bees are an important model for the ecology and evolution of gut microbiomes. These harbor ancient, specific, beneficial microbiomes crucial pollinators. However, most research has concentrated on managed honeybees bumblebees in temperate zone. Here we used 16S rRNA gene sequencing to characterize wild neotropical bumblebee communities from Colombia. We also analyzed drivers microbiome structure across our data previously published bumblebees. Our results show that lineages not only retained their ancient bacterial symbionts during dispersal North America but prone major disruption, a shift is strongly associated with parasite infection. Finally, found much more structured by host phylogeny than geography, despite very different environmental conditions plant two regions.

Language: Английский

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Exposure to a fungicide for a field-realistic duration does not alter bumble bee fecal microbiota structure

Applied and Environmental Microbiology, Journal Year: 2024, Volume and Issue: 90(2)

Published: Jan. 19, 2024

Social bees are frequently exposed to pesticides when foraging on nectar and pollen. Recent research has shown that pesticide exposure not only impacts social bee host health but can also alter the community structure of gut microbiotas. However, most pesticide-bee microbiota interactions been conducted in honey bees; bumble bees, native North American pollinators, have received less attention and, due differences their ecology, may be certain for shorter durations than bees. Here, we examine how fungicide chlorothalonil a short, field-realistic duration alters fecal microbiotas (used as proxy microbiotas) performance. We expose small groups

Language: Английский

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Extracellular symbiont colonizes insect during embryo development

ISME Communications, Journal Year: 2024, Volume and Issue: 4(1)

Published: Jan. 1, 2024

Abstract Insects typically acquire their beneficial microbes early in development. Endosymbionts housed intracellularly are commonly integrated during oogenesis or embryogenesis, whereas extracellular only known to be acquired after hatching by immature instars such as larvae nymphs. Here, however, we report on an symbiont that colonizes its host embryo Tortoise beetles (Chrysomelidae: Cassidinae) digestive bacterial Stammera extracellularly within foregut symbiotic organs and ovary-associated glands ensure vertical transmission. We outline the initial stages of colonization observe although develop 3 days prior larval emergence, they remain empty until final 24 h Infection occurs timeframe hatching. By experimentally manipulating availability embryos egg, describe a 12-h developmental window governing Stammera. Symbiotic form normally aposymbiotic larvae, demonstrating these Stammera-bearing structures autonomously. In adults, already colonized following metamorphosis stable population facilitate folivory. The glands, initially lack Symbiont abundance subsequently increases transmission organs, thereby ensuring sufficient titers at onset oviposition ~29 metamorphosis. Collectively, our findings reveal precedes where proliferation is eventually decoupled adult match nutritional reproductive requirements host.

Language: Английский

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A review on recent taxonomic updates of gut bacteria associated with social bees, with a curated genomic reference database

Insect Science, Journal Year: 2024, Volume and Issue: unknown

Published: April 9, 2024

Honeybees and bumblebees play a crucial role as essential pollinators. The special gut microbiome of social bees is key factor in determining the overall fitness health host. Although harbor relatively simple microbial communities at genus level, recent studies have unveiled significant genetic divergence variations gene content within each bacterial genus. However, comprehensive refined genomics-based taxonomic database specific to bee microbiomes remains lacking. Here, we first provided an overview current knowledge on distribution function bacteria, well factors that influence population dynamics. We then consolidated all available genomes bacteria species-level taxonomy, by constructing maximum-likelihood core genome phylogeny calculating genome-wide pairwise average nucleotide identity. On basis species constructed curated genomic reference database, named microbe sequence (BGM-GDb). To evaluate species-profiling performance BGM-GDb, retrieved series metagenomic data inferred composition using intra-species diversity analysis system (MIDAS), compared results with those obtained from prebuilt MIDAS database. found default BGM-GDb excelled aligned read counts richness. Overall, this high-resolution precise will facilitate research understanding community structure bees.

Language: Английский

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