A CRISPR view on genetic screens in Toxoplasma gondii DOI Creative Commons
Franziska Hildebrandt, Ana Catarina Matias, Moritz Treeck

et al.

Current Opinion in Microbiology, Journal Year: 2025, Volume and Issue: 83, P. 102577 - 102577

Published: Jan. 8, 2025

Genome editing technologies, such as CRISPR-Cas9, have revolutionised the study of genes in a variety organisms, including unicellular parasites. Today, CRISPR-Cas9 technology is vastly applied high-throughput screens to investigate interactions between Apicomplexan parasite Toxoplasma gondii and its hosts. In vitro vivo T. performed naive restrictive conditions led discovery essential fitness-conferring genes, well factors important for virulence dissemination. Recent studies adapted screening based on phenotypes unrelated survival. These advances were achieved by using conditional systems coupled with imaging, single-cell RNA sequencing phenotypic selection. Here, we review state-of-the-art technologies focus gondii, highlighting strengths, current limitations future avenues development, application other species.

Language: Английский

A CRISPR view on genetic screens in Toxoplasma gondii DOI Creative Commons
Franziska Hildebrandt, Ana Catarina Matias, Moritz Treeck

et al.

Current Opinion in Microbiology, Journal Year: 2025, Volume and Issue: 83, P. 102577 - 102577

Published: Jan. 8, 2025

Genome editing technologies, such as CRISPR-Cas9, have revolutionised the study of genes in a variety organisms, including unicellular parasites. Today, CRISPR-Cas9 technology is vastly applied high-throughput screens to investigate interactions between Apicomplexan parasite Toxoplasma gondii and its hosts. In vitro vivo T. performed naive restrictive conditions led discovery essential fitness-conferring genes, well factors important for virulence dissemination. Recent studies adapted screening based on phenotypes unrelated survival. These advances were achieved by using conditional systems coupled with imaging, single-cell RNA sequencing phenotypic selection. Here, we review state-of-the-art technologies focus gondii, highlighting strengths, current limitations future avenues development, application other species.

Language: Английский

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