IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco DOI Open Access
Chong Wang, Lianjun Wang,

Jian Lei

et al.

Genes, Journal Year: 2022, Volume and Issue: 13(8), P. 1476 - 1476

Published: Aug. 18, 2022

The MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factor family plays an important role in plant growth, development, and response to biotic abiotic stresses. However, the gene functions of factors sweet potato (Ipomoea batatas (L.) Lam) have not been elucidated. In this study, gene, IbMYB308, was identified isolated from potato. Multiple sequence alignment showed that IbMYB308 is a typical R2R3-MYB factor. Further, quantitative real-time PCR (qRT-PCR) analysis revealed expressed root, stem, and, especially, leaf tissues. Moreover, it had tissue-specific profile. experiment also expression induced by different stresses (20% PEG-6000, 200 mM NaCl, 20% H2O2). After NaCl treatment, several stress-related genes (SOD, POD, APX, P5CS) upregulation transgenic plants, CAT activity, POD proline content, protein content tobacco increased, while MDA decreased. conclusion, study demonstrated could improve salt stress tolerance tobacco. These findings lay foundation for future studies on suggest potentially be used as positive breeding plants.

Language: Английский

A lncRNA fine-tunes salicylic acid biosynthesis to balance plant immunity and growth DOI Creative Commons
Ningkun Liu,

Yanzhuo Xu,

Qi Li

et al.

Cell Host & Microbe, Journal Year: 2022, Volume and Issue: 30(8), P. 1124 - 1138.e8

Published: July 30, 2022

Constitutive activation of plant immunity is detrimental to growth and development. Here, we uncover the role a long non-coding RNA (lncRNA) in fine-tuning balance growth. We find that lncRNA termed salicylic acid biogenesis controller 1 (SABC1) suppresses promotes healthy plants. SABC1 recruits polycomb repressive complex 2 its neighboring gene NAC3, which encodes NAC transcription factor, decrease NAC3 via H3K27me3. activates isochorismate synthase (ICS1), key enzyme catalyzing (SA) biosynthesis. thus represses SA production decreasing ICS1 transcriptions. Upon pathogen infection, downregulated derepress resistance bacteria viruses. Together, our findings reveal as molecular switch balancing defense by modulating

Language: Английский

Citations

78
Linking discoveries, mechanisms, and technologies to develop a clearer perspective on plant long noncoding RNAs DOI Creative Commons
Kyle Palos, Liang Yu, Caylyn E Railey

et al.

The Plant Cell, Journal Year: 2023, Volume and Issue: 35(6), P. 1762 - 1786

Published: Feb. 4, 2023

Abstract Long noncoding RNAs (lncRNAs) are a large and diverse class of genes in eukaryotic genomes that contribute to variety regulatory processes. Functionally characterized lncRNAs play critical roles plants, ranging from regulating flowering controlling lateral root formation. However, findings the past decade have revealed thousands present plant transcriptomes, characterization has lagged far behind identification. In this setting, distinguishing function noise is challenging. community been at forefront discovery lncRNA biology, providing many functional mechanistic insights increased our understanding gene class. review, we examine key discoveries made biology over two half decades. We describe how pregenomics era informed efforts identify functionally characterize subsequent provide an overview archetypes into which fit speculate on new avenues research may uncover yet more archetypes. Finally, review discusses challenges facing field some exciting molecular computational approaches help inform comparative analyses.

Language: Английский

Citations

46
The Emerging Role of Non-Coding RNAs (ncRNAs) in Plant Growth, Development, and Stress Response Signaling DOI Creative Commons
Amit Kumar Yadav, Jyotirmaya Mathan, Arvind Kumar Dubey

et al.

Non-Coding RNA, Journal Year: 2024, Volume and Issue: 10(1), P. 13 - 13

Published: Feb. 7, 2024

Plant species utilize a variety of regulatory mechanisms to ensure sustainable productivity. Within this intricate framework, numerous non-coding RNAs (ncRNAs) play crucial role in plant biology, surpassing the essential functions RNA molecules as messengers, ribosomal, and transfer RNAs. ncRNAs represent an emerging class regulators, operating directly form small interfering (siRNAs), microRNAs (miRNAs), long noncoding (lncRNAs), circular (circRNAs). These exert control at various levels, including transcription, post-transcription, translation, epigenetic. Furthermore, they interact with each other, contributing biological processes associated stress resilience. This review primarily concentrates on recent advancements ncRNAs, delineating their growth development across organs such root, leaf, seed/endosperm, seed nutrient development. Additionally, broadens its scope by examining response environmental stresses drought, salt, flood, heat, cold plants. compilation offers updated information insights guide characterization potential growth, development, resilience future research.

Language: Английский

Citations

25
LncRNAs: the art of being influential without protein DOI Creative Commons
Lorena Ramírez Gonzales, Suze Blom, Rossana Henriques

et al.

Trends in Plant Science, Journal Year: 2024, Volume and Issue: 29(7), P. 770 - 785

Published: Feb. 16, 2024

The plant long noncoding (lnc)RNA field is on the brink of transitioning from large-scale identification lncRNAs to their functional characterization. Due cross-kingdom conservation interaction types and molecular functions, there much be learned mammalian lncRNA research. Here, we discuss different processes involving regulation chromatin splicing. Furthermore, interactome, which includes proteins, other RNAs, DNA. We explore how functionalities could reflected in similar pathways plants hypothesize that several breakthroughs research lead discovery novel functions. Expanding our knowledge biological role multiple applications paves way for future agricultural applications.

Language: Английский

Citations

17
Long Non-coding RNA in Plants in the Era of Reference Sequences DOI Creative Commons
Hikmet Budak,

Sezgi Kaya,

H. Busra Cagirici

et al.

Frontiers in Plant Science, Journal Year: 2020, Volume and Issue: 11

Published: March 12, 2020

The discovery of non-coding RNAs (ncRNAs), and the subsequent elucidation their functional roles, was largely delayed due to misidentification non-protein-coding parts DNA as "junk DNA," which forced ncRNAs into shadows protein-coding counterparts. However, over past decade, insight important regulatory roles has led rapid progress in identification characterization. Of different types ncRNAs, long (lncRNAs), attracted considerable attention mRNA-like structures gene functions plant stress responses. While RNA sequencing been commonly used for mining lncRNAs, a lack widespread conservation at sequence level addition relatively low highly tissue-specific expression patterns challenges high-throughput silico approaches. complex folding characteristics lncRNA molecules also complicate target predictions, knowledge about interaction interfaces between lncRNAs potential targets is insufficient. Progress characterizing from species may hold key efficient this class transcriptomic potentially genomic resources. In wheat barley, two most crops, very limited. recently published high-quality genomes these crops are considered promising resources not only but any molecules. Considering increasing demand food, should be efficiently discover molecular mechanisms lying behind development a/biotic As our understanding expands, interactions among ncRNA classes, well with coding sequences, will likely define novel networks that modulated crop improvement.

Language: Английский

Citations

126
Systematic identification of long noncoding RNAs expressed during light‐induced anthocyanin accumulation in apple fruit DOI Open Access
Tuo Yang, Huaying Ma, Jie Zhang

et al.

The Plant Journal, Journal Year: 2019, Volume and Issue: 100(3), P. 572 - 590

Published: July 25, 2019

Anthocyanin pigments contribute to the red color of apple (Malus × domestica) fruit and have a major influence on their ornamental, dietary market value. In this study, we investigated potential role long noncoding RNAs (lncRNAs) in anthocyanin biosynthesis. RNA-seq analysis peels from 'Red Fuji' cultivar during light-induced rapid accumulation revealed 5297 putative lncRNAs. Differential expression further showed that lncRNAs were induced light treatment involved photosynthesis. Using miRNA-lncRNA-mRNA network endogenous target mimic (eTM) analysis, predicted two differentially expressed lncRNAs, MLNC3.2 MLNC4.6, eTMs for miRNA156a promoted SPL2-like SPL33 transcription factors. Transient stable transformation callus overexpression SPLs accumulation, with opposite results eTM SPL-silenced fruit. Silencing or overexpressing miR156a also affected identified SPLs. These indicated MLNC4.6 function as prevent cleavage by Our study provides fundamental insights into lncRNA involvement biosynthetic pathway

Language: Английский

Citations

122
Classification and experimental identification of plant long non-coding RNAs DOI
Muneeza Iqbal, Maheen Alam, David A. Lightfoot

et al.

Genomics, Journal Year: 2018, Volume and Issue: 111(5), P. 997 - 1005

Published: April 19, 2018

Language: Английский

Citations

114
Long non-coding RNAs in plants: emerging modulators of gene activity in development and stress responses DOI Creative Commons
Li Chen, Qian‐Hao Zhu, Kerstin Kaufmann

et al.

Planta, Journal Year: 2020, Volume and Issue: 252(5)

Published: Oct. 24, 2020

Abstract Main conclusion Long non-coding RNAs modulate gene activity in plant development and stress responses by various molecular mechanisms. (lncRNAs) are transcripts larger than 200 nucleotides without protein coding potential. Computational approaches have identified numerous lncRNAs different species. Research the past decade has unveiled that participate a wide range of biological processes, including regulation flowering time morphogenesis reproductive organs, as well abiotic biotic responses. LncRNAs execute their functions interacting with DNA, RNA molecules, modulating expression level targets through epigenetic, transcriptional, post-transcriptional or translational regulation. In this review, we summarize characteristics lncRNAs, discuss recent progress on understanding lncRNA functions, propose an experimental framework for functional characterization.

Language: Английский

Citations

91
Research Progress on Plant Long Non-Coding RNA DOI Creative Commons

Ling Wu,

Sian Liu,

Haoran Qi

et al.

Plants, Journal Year: 2020, Volume and Issue: 9(4), P. 408 - 408

Published: March 25, 2020

Non-coding RNAs (ncRNAs) that were once considered “dark matter” or “transcriptional noise” in genomes are research hotspots the field of epigenetics. The most well-known microRNAs (miRNAs) a class short non-coding, small molecular weight with lengths 20–24 nucleotides highly conserved throughout evolution. Through complementary pairing bases target sites, gene transcripts cleaved and degraded, translation is inhibited, thus regulating growth development organisms. Unlike miRNAs, which have been studied thoroughly, long non-coding (lncRNAs) group poorly RNA molecules sequence length more than 200 no protein encoding capability; they interact large molecules, such as DNA, RNA, proteins, regulate modification, chromatin remodeling, functional activity, metabolism vivo through cis- trans-activation at transcriptional, post-transcriptional, epigenetic levels. Research on plant lncRNAs just beginning has gradually emerged biology. Currently, some studies revealed extensively involved stress response processes by mediating transmission expression genetic information. This paper systematically introduces lncRNA its regulatory mechanisms, reviews current status progress plants, summarizes main techniques strategies recent years, discusses existing problems prospects, order to provide ideas for further exploration verification specific evolution their biological functions.

Language: Английский

Citations

90
Sl‐lncRNA15492 interacts with Sl‐miR482a and affects Solanum lycopersicum immunity against Phytophthora infestans DOI Open Access
Ning Jiang, Jun Cui,

Xinxin Hou

et al.

The Plant Journal, Journal Year: 2020, Volume and Issue: 103(4), P. 1561 - 1574

Published: May 20, 2020

Long non-coding RNAs (lncRNAs) are involved in the resistance of plants to infection by pathogens via interactions with microRNAs (miRNAs). cleaved miRNAs produce phased small interfering (phasiRNAs), which, as competing endogenous (ceRNAs), function decoys for mature miRNAs, thus inhibiting their expression, and contain pre-miRNA sequences miRNAs. However, whether lncRNAs mediate other molecular mechanisms during plant is unknown. In this study, a positive regulator, Sl-lncRNA15492 from tomato (Solanum lycopersicum Zaofen No. 2) affected Phytophthora infestans. Gain- loss-of-function experiments RNA ligase-mediated 5'-amplification cDNA ends (RLM-5' RACE) also revealed that Sl-miR482a was negatively targeting Sl-NBS-LRR genes silencing Sl-NBS-LRR1 decreased resistance. inhibited expression Sl-miR482a, whose precursor located within antisense sequence Sl-lncRNA15492. Further degradome analysis additional RLM-5' RACE verified could cleave These results provide mechanism which might inhibit miRNA through strands lncRNAs, demonstrate mutually maintenance homeostasis P.

Language: Английский

Citations

87