Conifers concentrate large numbers of NLR immune receptor genes on one chromosome DOI Creative Commons
Yannick Woudstra, Hayley R. Tumas, Cyril Van Ghelder

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 23, 2023

Abstract Nucleotide-binding domain and Leucine-rich Repeat (NLR) immune receptor genes form a major line of defence in plants, acting both pathogen recognition resistance machinery activation. NLRs are reported to large gene clusters limber pine ( Pinus flexilis ) but it is unknown how widespread this genomic architecture may be among the extant species conifers (Pinophyta). We used comparative analyses assess patterns abundance, diversity distribution NLR genes. Chromosome-level whole genome assemblies high-density linkage maps Pinaceae, Cupressaceae, Taxaceae other gymnosperms were scanned for using existing customised pipelines. Discovered mapped across chromosomes groups, analysed phylogenetically evolutionary history. Conifer genomes characterised by dense genes, highly localised on one chromosome. These rich TNL-encoding which seem have formed through multiple tandem duplication events. In contrast angiosperms non-coniferous gymnosperms, clustering ubiquitous conifers. NLR-dense regions likely influence part plant’s resistance, informing our understanding adaptation biotic stress development genetic resources breeding. Plain language summary important pest, disease drought plants. giga-genomes conifers, they concentrate very small chromosomal regions. act as reservoirs can breeding improve resilience conifer trees.

Language: Английский

Cell death in bryophytes: emerging models to study core regulatory modules and conserved pathways DOI

Fernanda Marchetti,

Ayelén Mariana Distefano,

Maximiliano Cainzos

et al.

Annals of Botany, Journal Year: 2024, Volume and Issue: 134(3), P. 367 - 384

Published: Aug. 22, 2024

Abstract This review summarizes recent progress in our current understanding of the mechanisms underlying cell death pathways bryophytes, focusing on conserved and particularities comparison to angiosperms. Regulated (RCD) plays key roles during essential processes along plant life cycle. It is part specific developmental programmes maintains homeostasis organism response unfavourable environments. Bryophytes could provide valuable models study RCD as well those triggered by biotic abiotic stresses. Some analogous present angiosperms occur gametophytic haploid generation allowing direct genetic studies. In this review, we focus such programmes, identifying core raising new questions analyse from an evolutionary perspective.

Language: Английский

Citations

2
Bryo-FIGHTs: Emerging insights and principles acquired from non-vascular plant-pathogen interactions DOI Creative Commons

Hyeon‐Min Jeong,

Henrietta Patterson, Philip Carella

et al.

Current Opinion in Plant Biology, Journal Year: 2023, Volume and Issue: 76, P. 102484 - 102484

Published: Nov. 4, 2023

Since the dawn of land plant evolution, pathogenic microbes have impacted health and threatened their survival. Though much our knowledge on plant-pathogen interactions is derived from flowering plants, emerging research leveraging evolutionarily divergent non-vascular/non-seed bryophytes beginning to shed light history diversity immune infection processes. Here, we highlight key bryophyte-microbe pathosystems used address fundamental questions health. To this end, outline idea that core molecular aspects impacting immunity are likely conserved across plants. We discuss recent advances in field Evo-MPMI (evolutionary plant-microbe interactions) future opportunities will clarify understanding evolutionary framework underpins host-pathogen full spectrum evolution.

Language: Английский

Citations

4
Helper NLR immune protein NRC3 evolved to evade inhibition by a cyst nematode virulence effector DOI Creative Commons
Yu Sugihara, Jiorgos Kourelis, Mauricio P. Contreras

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 16, 2024

ABSTRACT Parasites can counteract host immunity by suppressing nucleotide binding and leucine-rich repeat (NLR) proteins that function as immune receptors. We previously showed a cyst nematode virulence effector SPRYSEC15 (SS15) binds inhibits oligomerisation of helper NLR in the expanded NRC1/2/3 clade preventing intramolecular rearrangements required for NRC into an activated resistosome. Here we examined degree to which from multiple Solanaceae species are sensitive suppression SS15 tested hypotheses about adaptive evolution interface between inhibitor proteins. Whereas all orthologs NRC2 were inhibited SS15, some natural variants NRC1 NRC3 insensitive suppression. Ancestral sequence reconstruction combined with functional assays revealed transitioned ancestral suppressed form one over 19 million years ago. Our analyses evolutionary trajectory coevolution parasite its receptor target, identifying key transitions NLRs this inhibition. This work reveals distinct type gene-for-gene interaction or pathogen immunosuppressors receptors contrasts AVR effectors

Language: Английский

Citations

1
A leucine-rich repeat receptor-like protein kinase enhances tomato resistance to Phelipanche aegyptiaca DOI
Lu Zhang, Zhaoqun Yao, Xiaolei Cao

et al.

Scientia Horticulturae, Journal Year: 2024, Volume and Issue: 337, P. 113353 - 113353

Published: Aug. 14, 2024

Language: Английский

Citations

1
PP2C phosphatase Pic14 negatively regulates tomato Pto/Prf-triggered immunity by inhibiting MAPK activation DOI Creative Commons
Joydeep Chakraborty, Guy Sobol, Fan Xia

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 4, 2024

SUMMARY Type 2C protein phosphatases (PP2Cs) are emerging as important regulators of plant immune responses, although little is known about how they might impact nucleotide-binding, leucine-rich repeat (NLR)-triggered immunity (NTI). We discovered that expression the PP2C-immunity associated candidate 14 gene ( Pic14 ) induced upon activation Pto/Prf-mediated NTI response in tomato. Pto/Prf recognize effector AvrPto translocated into cells by pathogen Pseudomonas syringae pv. tomato Pst and activate a MAPK cascade other responses which together confer resistance to bacterial speck disease. encodes PP2C with an N-terminal kinase-interacting motif (KIM) C-terminal phosphatase domain. Upon inoculation -AvrPto, Pto/Prf-expressing plants loss-of-function mutations developed less disease, specifically older leaves, compared wild-type plants. Transient leaves Nicotiana benthamiana inhibited cell death typically members M3Kα Mkk2. The death-suppressing activity was dependent on KIM catalytic M3Kα- Mkk2-mediated immunity-associated MAPKs shown be active physically interacts dephosphorylates Mkk2 KIM-dependent manner. Together, our results reveal negative regulator Pto/Prf-triggered interacting dephosphorylating SIGNIFICANCE STATEMENT Plant intracellular receptors, proteins (NLRs) such Prf NLR-triggered (NTI) specific virulence proteins. This paper reveals key signaling component acting downstream Pto/Prf, likely moderate effects growth or processes.

Language: Английский

Citations

0
Coordinated function of paired NLRs confersYr84-mediated stripe rust resistance in wheat DOI Creative Commons
Valentyna Klymiuk, Krystalee Wiebe, Harmeet Singh Chawla

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: June 17, 2024

Abstract Cloning of resistance genes expands our understanding their function and facilitates deployment in breeding. Here, we report the cloning two from wild emmer wheat ( Triticum turgidum ssp. dicoccoides ) underlying Yr84 -mediated stripe rust using a combination fine mapping, long read-sequencing mutation-induced functional validation. In contrast to all previously cloned genes, incompletely dominant phenotype is conferred through coordinated paired nucleotide-binding leucine-rich repeats (NLR) CNL NL . We hypothesize that based on genomic organization, annotation, expression profiles predicted protein structure, functions as sensor NLR (sNLR) responsible for effector recognition, acts helper (hNLR) initiating downstream cascades. The lack an integrated domain(s) implicated recognition by NLRs, therefore these findings contribute new insights into plant NLRs structure molecular mechanisms function.

Language: Английский

Citations

0
PP2C phosphatase Pic14 negatively regulates tomato Pto/Prf‐triggered immunity by inhibiting MAPK activation DOI
Joydeep Chakraborty, Guy Sobol, Fan Xia

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 119(6), P. 2622 - 2637

Published: July 20, 2024

SUMMARY Type 2C protein phosphatases (PP2Cs) are emerging as important regulators of plant immune responses, although little is known about how they might impact nucleotide‐binding, leucine‐rich repeat (NLR)‐triggered immunity (NTI). We discovered that expression the PP2C immunity‐associated candidate 14 gene ( Pic14 ) induced upon activation Pto/Prf‐mediated NTI response in tomato. Pto/Prf recognizes effector AvrPto translocated into cells by pathogen Pseudomonas syringae pv. tomato Pst and activate a MAPK cascade other responses which together confer resistance to bacterial speck disease. encodes with an N‐terminal kinase‐interacting motif (KIM) C‐terminal phosphatase domain. Upon inoculation ‐AvrPto, Pto/Prf‐expressing plants loss‐of‐function mutations developed less disease, specifically older leaves, compared wild‐type plants. Transient leaves Nicotiana benthamiana inhibited cell death typically members M3Kα Mkk2. The death‐suppressing activity was dependent on KIM catalytic M3Kα‐ Mkk2‐mediated MAPKs shown be active physically interacts dephosphorylates Mkk2 KIM‐dependent manner. Together, our results reveal negative regulator Pto/Prf‐triggered interacting dephosphorylating

Language: Английский

Citations

0
Advances in understanding the interaction between Solanaceae NLR resistance proteins and the viral effector Avr DOI Creative Commons

Jianming Wei,

Yunzhou Li, Xiangru Chen

et al.

Plant Signaling & Behavior, Journal Year: 2024, Volume and Issue: 19(1)

Published: Sept. 23, 2024

The rising prevalence of viral-induced diseases, particularly those caused by certain strains, poses a substantial risk to the genetic diversity

Language: Английский

Citations

0
Identification of the HbZAR1 Gene and Its Potential Role as a Minor Gene in Response to Powdery Mildew and Anthracnose of Hevea brasiliensis DOI Open Access
Qifeng Liu, Ang Qiao, Shihao Zhou

et al.

Forests, Journal Year: 2024, Volume and Issue: 15(11), P. 1891 - 1891

Published: Oct. 26, 2024

Powdery mildew and anthracnose are the main diseases of rubber trees. In recent years, there have been large outbreaks in rubber-planting areas Asia, seriously affecting yield quality latex. ZAR1 is a conserved distinctive coiled-coil nucleotide-binding leucine-rich (CNL) repeat plant kingdom, playing crucial role disease-resistance processes. To elucidate function HbZAR1 gene trees (Hevea brasiliensis), three candidate genes were identified using bioinformatics methods comprehensively analyzed. The results indicate that protein different species. Examination cis-regulatory element sequences HbZAR1genes reveals promoter exhibits remarkable enrichment stress, light, hormone elements. An expression analysis shows levels highest bark lowest Three can respond to both tree Erysiphe quercicola Colletotrichum siamense infection; especially, HbZAR1.1 HbZAR1.2 show significant upregulation during early stages infection. These findings suggest may be involved susceptibility E. C. through immune mechanisms. Subcellular localization expressed nucleus plasma membrane. This study also activated mutant HbZAR1.1D481V do not induce stable ROS production cell death, suggesting possible degradation, functional redundancy, or acting as minor disease resistance. research provides valuable insights for further studying mechanisms molecules.

Language: Английский

Citations

0
Conifers concentrate large numbers of NLR immune receptor genes on one chromosome DOI Creative Commons
Yannick Woudstra, Hayley R. Tumas, Cyril Van Ghelder

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 23, 2023

Abstract Nucleotide-binding domain and Leucine-rich Repeat (NLR) immune receptor genes form a major line of defence in plants, acting both pathogen recognition resistance machinery activation. NLRs are reported to large gene clusters limber pine ( Pinus flexilis ) but it is unknown how widespread this genomic architecture may be among the extant species conifers (Pinophyta). We used comparative analyses assess patterns abundance, diversity distribution NLR genes. Chromosome-level whole genome assemblies high-density linkage maps Pinaceae, Cupressaceae, Taxaceae other gymnosperms were scanned for using existing customised pipelines. Discovered mapped across chromosomes groups, analysed phylogenetically evolutionary history. Conifer genomes characterised by dense genes, highly localised on one chromosome. These rich TNL-encoding which seem have formed through multiple tandem duplication events. In contrast angiosperms non-coniferous gymnosperms, clustering ubiquitous conifers. NLR-dense regions likely influence part plant’s resistance, informing our understanding adaptation biotic stress development genetic resources breeding. Plain language summary important pest, disease drought plants. giga-genomes conifers, they concentrate very small chromosomal regions. act as reservoirs can breeding improve resilience conifer trees.

Language: Английский

Citations

1