Integration of fungal transcriptomics and metabolomics provides insights into the early interaction between the ORM fungus Tulasnella sp. and the orchid Serapias vomeracea seeds DOI Creative Commons
Silvia De Rose, Fabiano Sillo, Andrea Ghirardo

et al.

IMA Fungus, Journal Year: 2024, Volume and Issue: 15(1)

Published: Oct. 25, 2024

In nature, germination of orchid seeds and early plant development rely on a symbiotic association with mycorrhizal (ORM) fungi. These fungi provide the host necessary nutrients facilitate transition from embryos to protocorms. Despite recent advances in omics technologies, our understanding this symbiosis remains limited, particularly during initial stages interaction. To address gap, we employed transcriptomics metabolomics investigate responses occurring fungus Tulasnella sp. isolate SV6 when co-cultivated Serapias vomeracea. The integration data gene expression metabolite profiling revealed activation some fungal signalling pathways before establishment symbiosis. Prior seed contact, an indole-related was produced by fungus, significant changes lipid profile occurred throughout process. Additionally, cell wall-degrading enzymes (PCWDEs) observed pre-symbiotic stage, as approached seeds, along amino acid metabolism. Thus, dual-omics approach study yielded novel insights into relationship between orchids ORM suggest that responds presence prior contact.

Language: Английский

The mycorrhizal symbiosis: research frontiers in genomics, ecology, and agricultural application DOI Open Access
Francis Martin, Marcel G. A. van der Heijden

New Phytologist, Journal Year: 2024, Volume and Issue: 242(4), P. 1486 - 1506

Published: Jan. 31, 2024

Summary Mycorrhizal symbioses between plants and fungi are vital for the soil structure, nutrient cycling, plant diversity, ecosystem sustainability. More than 250 000 species associated with mycorrhizal fungi. Recent advances in genomics related approaches have revolutionized our understanding of biology ecology associations. The genomes 250+ been released hundreds genes that play pivotal roles regulating symbiosis development metabolism characterized. rDNA metabarcoding metatranscriptomics provide novel insights into ecological cues driving communities functions expressed by these associations, linking to traits such as acquisition organic matter decomposition. Here, we review genomic studies revealed involved uptake development, discuss adaptations fundamental evolution lifestyles. We also evaluated services provided networks how hold promise sustainable agriculture forestry enhancing stress tolerance. Overall, unraveling intricate dynamics is paramount promoting sustainability addressing current pressing environmental concerns. This ends major frontiers further research.

Language: Английский

Citations

62
Symbiotic synergy: How Arbuscular Mycorrhizal Fungi enhance nutrient uptake, stress tolerance, and soil health through molecular mechanisms and hormonal regulation DOI Creative Commons
Nazir Ahmed, Juan Li, Yongquan Li

et al.

IMA Fungus, Journal Year: 2025, Volume and Issue: 16

Published: March 21, 2025

Arbuscular Mycorrhizal (AM) symbiosis is integral to sustainable agriculture and enhances plant resilience abiotic biotic stressors. Through their symbiotic association with roots, AM improves nutrient water uptake, activates antioxidant defenses, facilitates hormonal regulation, contributing improved health productivity. Plants release strigolactones, which trigger spore germination hyphal branching, a process regulated by genes, such as D27 , CCD7 CCD8 MAX1 . recognition plants mediated receptor-like kinases (RLKs) LysM domains, leading the formation of arbuscules that optimize exchange. Hormonal regulation plays pivotal role in this symbiosis; cytokinins enhance colonization, auxins support arbuscule formation, brassinosteroids regulate root growth. Other hormones, salicylic acid, gibberellins, ethylene, jasmonic abscisic also influence colonization stress responses, further bolstering resilience. In addition health, soil improving microbial diversity, structure, cycling, carbon sequestration. This supports pH pathogen suppression, offering alternative chemical fertilizers fertility. To maximize ’s potential agriculture, future research should focus on refining inoculation strategies, enhancing compatibility different crops, assessing long-term ecological economic benefits. Optimizing applications critical for agricultural resilience, food security, farming practices.

Language: Английский

Citations

2
Mycoheterotrophy in the wood-wide web DOI
Vincent S. F. T. Merckx, Sofia I. F. Gomes, Deyi Wang

et al.

Nature Plants, Journal Year: 2024, Volume and Issue: 10(5), P. 710 - 718

Published: April 19, 2024

Language: Английский

Citations

12
Perspective and challenges of mycorrhizal symbiosis in orchid medicinal plants DOI Open Access

Chun-Yan Leng,

Mengyan Hou,

Yong-Mei Xing

et al.

Chinese Herbal Medicines, Journal Year: 2024, Volume and Issue: 16(2), P. 172 - 179

Published: March 8, 2024

The family Orchidaceae is of the most diverse taxon in plant kingdom, and its members are highly valuable herbal medicines. Orchids have a unique mycorrhizal symbiotic relationship with specific fungi for carbohydrate nutrient supplies their whole lifecycle. large-scale cultivation medicinal Gastodia elata successful example using technology. In this review, we adopted G. Dendrobium officinale as examples to describe characteristics orchid mycorrhiza benefits host plants' growth health (e.g. biotic abiotic stress secondary metabolite accumulation). challenges applying technology plants future were also discussed. This review aims serve theoretical guide plants.

Language: Английский

Citations

5
Arbuscular mycorrhizal fungi DOI

Francesco Bergese,

Luca Giovannini, Fabiano Sillo

et al.

Elsevier eBooks, Journal Year: 2025, Volume and Issue: unknown, P. 171 - 188

Published: Jan. 1, 2025

Citations

0
Ecological divergence of sibling allopolyploid marsh orchids is associated with species specific plasticity and distinct fungal communities DOI Creative Commons
Katie Emelianova,

Anna‐Sophie Hawranek,

Mimmi C. Eriksson

et al.

The Plant Journal, Journal Year: 2025, Volume and Issue: 121(4)

Published: Feb. 1, 2025

SUMMARY Phenotypic plasticity, the dynamic adjustment of traits to environmental variations, is crucial for enabling species exploit broader niches and withstand suboptimal conditions. This adaptability particularly relevant newly formed allopolyploids, which possess redundant gene copies must become established in diverse environments distinct from their parents other relatives. By evaluating expression root mycobiome among two ecologically divergent sibling allopolyploid marsh orchids ( Dactylorhiza majalis D. traunsteineri ) reciprocal transplants at localities where both are native, we aimed understand drivers persistence face interspecific flow. Despite consistent abiotic differences characterising alternative each locality, majority between allopolyploids appears be plastic. Ecologically processes, such as photosynthesis transmembrane transport, include some genes that differentially expressed regardless environment, while others change activity plastically one or other. suggests although plasticity helps define specific ecological range allopolyploid, it also mediates flow them, thereby preventing differentiation. Extending our investigations mycobiome, uncover more fungal communities either when grown environment with nutrient‐poor soils, indicating biotic factors drive distribution orchids. Altogether, results indicate can simultaneously promote diversification homogenisation lineages, influencing establishment recurrently species.

Language: Английский

Citations

0
Soil cropping selects for nutrient efficient but more costly indigenous mycorrhizal fungal communities DOI Creative Commons
Paula A. Buil, Jan Jansa,

Martin Rozmoš

et al.

Biology and Fertility of Soils, Journal Year: 2025, Volume and Issue: unknown

Published: March 4, 2025

Language: Английский

Citations

0
Forest mycorrhizal types mediated environmental controls on global particulate and mineral-associated organic matter storage DOI

Luping Ma,

Zhaoyong Shi,

Manman Jing

et al.

Environmental Research, Journal Year: 2025, Volume and Issue: unknown, P. 121459 - 121459

Published: March 1, 2025

Language: Английский

Citations

0
SOC sequestration, N and P retention in mineral soils depend on arbuscular mycorrhizal tree dominance and soil microbial traits DOI

Qianru Ji,

Qi Li,

Yanbo Yang

et al.

Applied Soil Ecology, Journal Year: 2025, Volume and Issue: 211, P. 106136 - 106136

Published: April 23, 2025

Language: Английский

Citations

0
Omics approaches to investigate pre-symbiotic responses of the mycorrhizal fungus Tulasnella sp. SV6 to the orchid host Serapias vomeracea DOI Creative Commons
Silvia De Rose, Fabiano Sillo, Andrea Ghirardo

et al.

Mycorrhiza, Journal Year: 2025, Volume and Issue: 35(2)

Published: April 1, 2025

Like other plant-microbe symbioses, the establishment of orchid mycorrhiza (ORM) is likely to require specific communication and metabolic adjustments between two partners. However, while modulation plant fungal metabolism has been investigated in fully established mycorrhizal tissues, molecular changes occurring during pre-symbiotic stages interaction remain largely unexplored ORM. In this study, we responses ORM fungus Tulasnella sp. SV6 plantlets host Serapias vomeracea a dual vitro cultivation system. The mycelium was harvested prior physical contact with roots transcriptome metabolome were analyzed using RNA-seq untargeted metabolomics approaches. results revealed distinct transcriptomic metabolomic remodelling presence plantlets, as compared free-living condition. responds significant up-regulation genes associated protein synthesis, amino acid lipid biosynthesis, indicating increased activity. Metabolomic analysis supported data, showing levels acids phospholipids, suggesting cell structure signalling interaction. addition, identified an increase transcripts small secreted that may play role early symbiotic signalling. Taken together, our suggest perceive information from roots, leading readjustment its profiles.

Language: Английский

Citations

0