IgE-based analysis of sensitization and cross-reactivity to yellow mealworm and edible insect allergens before their widespread dietary introduction

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Jan. 9, 2025

The European Commission authorized the use of dried yellow mealworm (Tenebrio molitor - TM) as a food ingredient under Regulation EU 2021/882. As TM emerges an important allergen source, sensitization and allergy to in various populations need investigation. aim this study was assess incidence before its introduction Poland, well checking occurrence co-sensitivity other invertebrate allergenic extracts molecules. This analysis performed using serum allergen-specific immunoglobulin E (sIgE) results 6,173 individuals ALEX2 test detect sensitivity related allergens. A total 4.3% population had sIgE extract, with 0.7% those being mono-sensitized TM. Sensitization most commonly associated positive response house cricket migratory locust antibodies against significantly more (p < 0.001) co-occurred Patients were likely sensitised tropomyosins (49.1% patients), Niemann-Pick C2 protein (43.8%), group 5/21 (38.6%), class III chitinases (37.1%), cysteine proteases (34.1%). Based on asIgE levels prior introduced we hypothesised that primary may be allergies. Our showed sensitisation reaction dust mites shrimp tropomyosins. Therefore, speculate allergic should exercise caution when consuming foods containing

Language: Английский

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Hot topics in allergen immunotherapy, 2023: Current status and future perspective

Allergy, Journal Year: 2023, Volume and Issue: 79(4), P. 823 - 842

Published: Nov. 20, 2023

The importance of allergen immunotherapy (AIT) is multifaceted, encompassing both clinical and quality-of-life improvements cost-effectiveness in the long term. Key mechanisms tolerance induced by AIT include changes memory type allergen-specific T- B-cell responses towards a regulatory phenotype with decreased Type 2 responses, suppression IgE increased IgG

Language: Английский

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A systematic review of allergen cross-reactivity: Translating basic concepts into clinical relevance

Journal of Allergy and Clinical Immunology Global, Journal Year: 2024, Volume and Issue: 3(2), P. 100230 - 100230

Published: Feb. 19, 2024

Access to the molecular culprits of allergic reactions allows for leveraging allergology as a new precision medicine approach-one built on interdisciplinary, basic, and clinical knowledge. Molecular relies use allergen molecules

Language: Английский

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Introduction of a human- and keyboard-friendly N-glycan nomenclature

Beilstein Journal of Organic Chemistry, Journal Year: 2024, Volume and Issue: 20, P. 607 - 620

Published: March 15, 2024

In the beginning was word. But there were no words for N-glycans, at least, simple words. Next to chemical formulas, IUPAC code can be regarded as best, most reliable and yet immediately comprehensible annotation of oligosaccharide structures any type from source. When it comes venerable has, however, been widely supplanted by highly simplified terms N-glycans that count number antennae or certain components such galactoses, sialic acids fucoses give only limited room exact structure description. The illustrative – fortunately now standardized cartoon depictions gained much ground during last years. By their very nature, cartoons neither written nor spoken. underlying machine codes (e.g., GlycoCT, WURCS) are definitely not intended direct use in human communication. So, one might feel need a simple, intelligible precise system alphanumeric descriptions hundreds thousands N-glycan structures. Here, we present describes defining terminal elements. To minimize redundancy length terms, common elements taken granted. preset reading order facilitates definition positional isomers. combination with condensed allows describe even rather complex structural Thus, this “proglycan” coding could missing link between drawn software-oriented representations On top, may greatly facilitate keyboard-based mining glycan substructures repositories.

Language: Английский

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Multiplex basophil activation tests for allergy diagnosis: present and future applications

Frontiers in Allergy, Journal Year: 2025, Volume and Issue: 5

Published: Jan. 14, 2025

The basophil activation test (BAT) has become a major cellular in vitro for evaluating the allergenic activity of specific IgEs. impact BAT is due to ability blood granulocytes present IgE on high-affinity Fc ε RI receptor and mirror mast cell response that elicits an acute allergic reaction. proved be able identify patients at risk reacting low dose allergen and/or developing life-threatening reactions thus can significantly improve current management patients. However, diagnostic utility identifying different genuinely sensitizing allergens lower procedure labour requirements, multiplex approach incorporating multiple components would highly anticipated. Recently, novel was described utilizing two innovative steps. first step fluorescent labeling allergens. second applying fluorescently labeled flow cytometry assessment analyze subpopulations gated according binding or evaluate fluorescence intensity surface basophils. These approaches will advance our understanding IgE-mediated responses. Integration BAT, addition assays into practice, personalize measurement help estimate likelihood clinical relevance risks when testing individual

Language: Английский

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Precision engineering for localization, validation, and modification of allergenic epitopes

Journal of Allergy and Clinical Immunology, Journal Year: 2024, Volume and Issue: 153(3), P. 560 - 571

Published: Jan. 4, 2024

Language: Английский

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Understanding the heterogeneity of childhood allergic sensitization and its relationship with asthma

Current Opinion in Allergy and Clinical Immunology, Journal Year: 2024, Volume and Issue: 24(2), P. 79 - 87

Published: Feb. 7, 2024

Purpose of review To the current state knowledge on relationship between allergic sensitization and asthma; to lay out a roadmap for development IgE biomarkers that differentiate, in individual sensitized patients, whether their is important or future asthma symptoms, has little no relevance disease. Recent findings The evidence suggests some subtypes are not associated with whilst others pathologic. Interaction patterns antibodies allergenic molecules component-resolved diagnostics (CRD) multiplex arrays might be hallmarks by which different relevant can distinguished. These amongst individuals at all ages, clinical presentations (no disease, as single multimorbidity); preschool children without lower airway risk subsequent development; patients asthma, differing levels severity. Summary use machine learning-based methodologies complex CRD data help us design better diagnostic tools practising physicians differentiate benign clinically sensitization.

Language: Английский

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AAAAI–EAACI PRACTALL: Standardizing oral food challenges—2024 Update

Pediatric Allergy and Immunology, Journal Year: 2024, Volume and Issue: 35(11)

Published: Nov. 1, 2024

This common statement of the American Academy Allergy, Asthma and Immunology (AAAAI) The European Allergy Clinical (EAACI) provides an update 2012 published guidelines on food challenges. equally address challenges in research clinical settings. They first diagnostic tests which can guide decision to conduct a challenge. Safety is prime, various procedures safety issues as well medications potentially involved are extensively discussed. Challenges suggested be conducted with semi-logarithmic incremental doses based protein content, typically for IgE-mediated allergy intervals 20-30 min between doses. Specific protocols other types reactions such atopic dermatitis or gastrointestinal detailed separately. Proper stopping criteria essential order reduce risk false-positive diagnoses, but also severe reactions. recommend "go on," "stop," "observation." These revised will clearly provide much needed guidance continue evolve new needs field allergy.

Language: Английский

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The History and Science of the Major Birch Pollen Allergen Bet v 1

Biomolecules, Journal Year: 2023, Volume and Issue: 13(7), P. 1151 - 1151

Published: July 19, 2023

The term allergy was coined in 1906 by the Austrian scientist and pediatrician Clemens Freiherr von Pirquet. In 1976, Dietrich Kraft became head of Allergy Immunology Research Group at Department General Experimental Pathology University Vienna. 1983, proposed to replace natural extracts used diagnostic tests vaccines with recombinant allergen molecules persuaded Michael Breitenbach contribute his expertise molecular cloning as one mentors this project. Thus, foundation for Vienna School Molecular Allergology laid. With recruitment Heimo Breiteneder a young biology researcher, work began earnest, resulting publication first plant Bet v 1 1989. has become subject very large number basic scientific well clinical studies. is also founding member 1-like superfamily proteins members—based on ancient conserved fold—being present all three domains life, i.e., archaea, bacteria eukaryotes. This suggests that fold most likely already existed last universal common ancestor. biological function protein probably related lipid binding. However, during evolution, functional diversity within established. comprises 25 families, which family, turn composed 11 subfamilies. One these, PR-10-like subfamily proteins, contains almost homologous allergens from pollen foods. Structural comparisons its non-allergenic homologs will pave way deeper understanding allergic sensitization process.

Language: Английский

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Revisiting Fruit Allergy: Prevalence across the Globe, Diagnosis, and Current Management

Foods, Journal Year: 2023, Volume and Issue: 12(22), P. 4083 - 4083

Published: Nov. 10, 2023

Fruit allergies manifest with a diverse array of clinical presentations, ranging from localized contact and oral allergy syndrome to the potential for severe systemic reactions including anaphylaxis. The scope population-level prevalence studies remains limited, largely derived single-center or hospital-based investigations. In this comprehensive review, we conducted systematic literature search spanning years 2009 2023, full acknowledgment analytical biases, provide global overview fruit prevalence. primary mechanistic underpinning stems cross-reactivity between aeroallergens food allergens, consequence structurally similar epitopes—a phenomenon recognized as pollen (PFAS). era molecular allergology, numerous have dissected allergen components substantial relevance. Within explore important allergenic molecules found in plant-based foods, scrutinize pertinent patterns, offer insights into management recommendations. Additionally, compare guideline recommendations enhance understanding inform decision making.

Language: Английский

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