Principles, Applications, and Biosafety of Plant Genome Editing Using CRISPR-Cas9 DOI Creative Commons

Kaoutar El‐Mounadi,

María Luisa Morales-Floriano,

Hernán García-Ruíz

et al.

Frontiers in Plant Science, Journal Year: 2020, Volume and Issue: 11

Published: Feb. 13, 2020

The terms genome engineering, editing and gene editing, refer to modifications insertions, deletions, substitutions) in the of a living organism. most widely used approach nowadays is based on Clustered Regularly Interspaced Short Palindromic Repeats associated protein 9 (CRISPR-Cas9). In prokaryotes, CRISPR-Cas9 an adaptive immune system that naturally protects cells from DNA virus infections. has been modified create versatile technology wide diversity applications medicine, agriculture, basic studies functions. growing number monocot dicot plant species enhance yield, quality nutritional value, introduce or tolerance biotic abiotic stress, domestication, other applications. Although biosafety concerns remain, promising with potential contribute food production for benefit Gene This provisional file, not final typeset article human population. Here we review principles, current advances CRISPR-Cas9-based crop improvement. We also address show humans have exposed Cas9 homologues long before use CRISP-Cas9 editing.

Language: Английский

CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture DOI Open Access

Kunling Chen,

Yanpeng Wang,

Rui Zhang

et al.

Annual Review of Plant Biology, Journal Year: 2019, Volume and Issue: 70(1), P. 667 - 697

Published: March 5, 2019

Enhanced agricultural production through innovative breeding technology is urgently needed to increase access nutritious foods worldwide. Recent advances in CRISPR/Cas genome editing enable efficient targeted modification most crops, thus promising accelerate crop improvement. Here, we review CRISPR/Cas9 and its variants examine their applications plant related manipulations. We highlight base-editing tools that nucleotide substitutions describe the various delivery systems, particularly DNA-free methods, have linked with breeding. summarize of for trait improvement, development techniques fine-tuning gene regulation, strategies virus resistance, use high-throughput mutant libraries. outline future perspectives synthetic biology domestication, specificity, homology-directed repair, drives. Finally, discuss challenges opportunities precision bright agriculture.

Language: Английский

Citations

1244
Applications of CRISPR–Cas in agriculture and plant biotechnology DOI
Haocheng Zhu, Chao Li, Caixia Gao

et al.

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 21(11), P. 661 - 677

Published: Sept. 24, 2020

Language: Английский

Citations

648
CRISPR for Crop Improvement: An Update Review DOI Creative Commons

Deepa Jaganathan,

Karthikeyan Ramasamy,

Gothandapani Sellamuthu

et al.

Frontiers in Plant Science, Journal Year: 2018, Volume and Issue: 9

Published: July 17, 2018

The availability of genome sequences for several crops and advances in editing approaches has opened up possibilities to breed almost any given desirable trait. Advancements technologies such as zinc finger nucleases (ZFNs), transcription activator-like effector (TALENs) made it possible molecular biologists more precisely target gene interest. However, these methodologies are expensive time-consuming they involve complicated steps that require protein engineering. Unlike first-generation tools, CRISPR/Cas9 involves simple designing cloning methods, with the same Cas9 being potentially available use different guide RNAs targeting multiple sites genome. After proof-of-concept demonstrations crop plants involving primary CRISPR-Cas9 module, modified cassettes have been utilized improving specificity reducing off-target cleavage (e.g., Nmcas9, Sacas9, Stcas9). Further, enzymes from additional bacterial species options enhance efficiency methodologies. This review summarizes plant biotechnologists bring about improvement using based tools also presents studies where used enhancing biotic abiotic stress tolerance. Application techniques will result development non-genetically (Non-GMO) desired trait can contribute increased yield potential under conditions.

Language: Английский

Citations

561
The Enhancement of Plant Disease Resistance Using CRISPR/Cas9 Technology DOI Creative Commons

Virginia Maria Grazia Borrelli,

Vittoria Brambilla, Peter Rogowsky

et al.

Frontiers in Plant Science, Journal Year: 2018, Volume and Issue: 9

Published: Aug. 24, 2018

Genome editing technologies have progressed rapidly and become one of the most important genetic tools in implementation pathogen resistance plants. Recent years witnessed emergence site directed modification methods using meganucleases, zinc finger nucleases (ZFNs), transcription activator-like effector (TALENs), clustered regularly interspaced short palindrome repeats (CRISPR)/CRISPR-associated protein 9 (Cas9). Recently, CRISPR/Cas9 has largely overtaken other genome due to fact that it is easier design implement, a higher success rate, more versatile less expensive. This review focuses on recent advances plant protection technology model plants crops response viral, fungal bacterial diseases. As regards achievement viral disease resistance, main strategies employed species such as Arabidopsis Nicotiana benthamiana, which include integration CRISPR-encoding sequences target interfere with induction CRISPR-mediated targeted mutation host genome, will be discussed. Furthermore, based susceptibility genes crop rice, tomato, wheat citrus reviewed. After spending deciphering reading genomes, researchers are now rewriting them develop resistant specific pests pathogens.

Language: Английский

Citations

457
CRISPR/Cas System: Recent Advances and Future Prospects for Genome Editing DOI Creative Commons
Hakim Manghwar, Keith Lindsey, Xianlong Zhang

et al.

Trends in Plant Science, Journal Year: 2019, Volume and Issue: 24(12), P. 1102 - 1125

Published: Nov. 11, 2019

Language: Английский

Citations

403
Applications and potential of genome editing in crop improvement DOI Creative Commons
Yi Zhang, Karen Massel, Ian D. Godwin

et al.

Genome biology, Journal Year: 2018, Volume and Issue: 19(1)

Published: Nov. 30, 2018

Genome-editing tools provide advanced biotechnological techniques that enable the precise and efficient targeted modification of an organism's genome. systems have been utilized in a wide variety plant species to characterize gene functions improve agricultural traits. We describe current applications genome editing plants, focusing on its potential for crop improvement terms adaptation, resilience, end-use. In addition, we review novel breakthroughs are extending genome-edited crops possibilities their commercialization. Future prospects integrating this revolutionary technology with conventional new-age breeding strategies also discussed.

Language: Английский

Citations

358
Gene Editing and Crop Improvement Using CRISPR-Cas9 System DOI Creative Commons
Leena Arora, Alka Narula

Frontiers in Plant Science, Journal Year: 2017, Volume and Issue: 8

Published: Nov. 8, 2017

Advancements in Genome editing technologies have revolutionized the fields of functional genomics and crop improvement. CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeat)-Cas9 technology is a versatile tool for genome engineering that uses guideRNA (gRNA) to target Cas9 endonuclease modify specific sequence. It has broadened agricultural research area, bringing new opportunities develop novel plant varieties with deletion detrimental traits or addition significant characters. This RNA guided turning out be groundbreaking innovation distinct branches biology. CRISPR constantly advancing including options various genetic manipulations like generating knockouts; making precise modifications, multiplex engineering, activation repression genes. The review highlights progression throughout legacy. We studied rapid evolution tools myriad functionalities, capabilities specialized applications. Among varied diligences, nutritional improvement, enhancement disease resistance production drought tolerant plants are reviewed. also includes some information on traditional delivery methods Cas9-gRNA complexes into cells incorporates advent ribonucleoproteins (RNPs) came up as solution limitations prevailed plasmid-based system.

Language: Английский

Citations

310
Xanthomonas diversity, virulence and plant–pathogen interactions DOI Open Access
Sujan Timilsina, Neha Potnis, Eric A. Newberry

et al.

Nature Reviews Microbiology, Journal Year: 2020, Volume and Issue: 18(8), P. 415 - 427

Published: April 28, 2020

Language: Английский

Citations

276
Genome Editing: Targeting Susceptibility Genes for Plant Disease Resistance DOI Creative Commons

Syed Shan‐e‐Ali Zaidi,

M. Shahid Mukhtar, Shahid Mansoor

et al.

Trends in biotechnology, Journal Year: 2018, Volume and Issue: 36(9), P. 898 - 906

Published: May 8, 2018

Language: Английский

Citations

275
Towards CRISPR/Cas crops – bringing together genomics and genome editing DOI Creative Commons
Armin Scheben, Felix Wolter, Jacqueline Batley

et al.

New Phytologist, Journal Year: 2017, Volume and Issue: 216(3), P. 682 - 698

Published: Aug. 1, 2017

Contents 682 I. II. 683 III. 684 IV. 685 V. VI. 688 VII. 690 VIII. 694 References SUMMARY: With the rapid increase in global population and impact of climate change on agriculture, there is a need for crops with higher yields greater tolerance to abiotic stress. However, traditional crop improvement via genetic recombination or random mutagenesis laborious process cannot keep pace increasing demand. Genome editing technologies such as clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated protein (CRISPR/Cas) allow targeted modification almost any genome sequence generate novel variation accelerate breeding efforts. We expect gradual shift away from towards cycles editing. Crop using not constrained by limited existing requirement select alleles over multiple generations. current applications are lack complete reference genomes, sparse knowledge potential targets, unclear legal status edited crops. argue that overcoming technical social barriers application will this technology produce new generation high-yielding, ready

Language: Английский

Citations

261