CRISPR/Cas Genome Editing and Precision Plant Breeding in Agriculture

Annual Review of Plant Biology, Journal Year: 2019, Volume and Issue: 70(1), P. 667 - 697

Published: March 5, 2019

Enhanced agricultural production through innovative breeding technology is urgently needed to increase access nutritious foods worldwide. Recent advances in CRISPR/Cas genome editing enable efficient targeted modification most crops, thus promising accelerate crop improvement. Here, we review CRISPR/Cas9 and its variants examine their applications plant related manipulations. We highlight base-editing tools that nucleotide substitutions describe the various delivery systems, particularly DNA-free methods, have linked with breeding. summarize of for trait improvement, development techniques fine-tuning gene regulation, strategies virus resistance, use high-throughput mutant libraries. outline future perspectives synthetic biology domestication, specificity, homology-directed repair, drives. Finally, discuss challenges opportunities precision bright agriculture.

Language: Английский

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Applications of CRISPR–Cas in agriculture and plant biotechnology

Nature Reviews Molecular Cell Biology, Journal Year: 2020, Volume and Issue: 21(11), P. 661 - 677

Published: Sept. 24, 2020

Language: Английский

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Applications and potential of genome editing in crop improvement

Genome biology, Journal Year: 2018, Volume and Issue: 19(1)

Published: Nov. 30, 2018

Genome-editing tools provide advanced biotechnological techniques that enable the precise and efficient targeted modification of an organism's genome. systems have been utilized in a wide variety plant species to characterize gene functions improve agricultural traits. We describe current applications genome editing plants, focusing on its potential for crop improvement terms adaptation, resilience, end-use. In addition, we review novel breakthroughs are extending genome-edited crops possibilities their commercialization. Future prospects integrating this revolutionary technology with conventional new-age breeding strategies also discussed.

Language: Английский

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Novel CRISPR–Cas Systems: An Updated Review of the Current Achievements, Applications, and Future Research Perspectives

International Journal of Molecular Sciences, Journal Year: 2021, Volume and Issue: 22(7), P. 3327 - 3327

Published: March 24, 2021

According to Darwin’s theory, endless evolution leads a revolution. One such example is the Clustered Regularly Interspaced Palindromic Repeats (CRISPR)–Cas system, an adaptive immunity system in most archaea and many bacteria. Gene editing technology possesses crucial potential dramatically impact miscellaneous areas of life, CRISPR–Cas represents suitable strategy. The has ignited revolution field genetic engineering. ease, precision, affordability this akin Midas touch for researchers genomes. Undoubtedly, applications are endless. extensively employed treatment infectious diseases, metabolic disorders, curing cancer, developing sustainable methods fuel production chemicals, improving quality quantity food crops, thus catering global demands. Future will provide benefits everyone save countless lives. evolving rapidly; therefore, overview continuous improvement important. In review, we aim elucidate current state tailor-made format from its discovery exciting breakthroughs at application level further upcoming trends related opportunities challenges including ethical concerns.

Language: Английский

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Highly efficient homology‐directed repair using CRISPR/Cpf1‐geminiviral replicon in tomato

Plant Biotechnology Journal, Journal Year: 2020, Volume and Issue: 18(10), P. 2133 - 2143

Published: March 16, 2020

Summary Genome editing via the homology‐directed repair (HDR) pathway in somatic plant cells is very inefficient compared with error‐prone by nonhomologous end joining (NHEJ). Here, we increased HDR‐based genome efficiency approximately threefold a Cas9‐based single‐replicon system use of de novo multi‐replicon systems equipped CRISPR/LbCpf1 tomato and obtained replicon‐free but stable HDR alleles. The CRISPR/LbCpf1‐based was significantly modulated physical culture conditions such as temperature light. Ten days incubation at 31 °C under light/dark cycle after Agrobacterium ‐mediated transformation resulted best performance among tested conditions. Furthermore, developed our into that effectively efficiency. Although this approach still challenging, showed feasibility salt‐tolerant SlHKT1;2 allele without genomic integration antibiotic markers or any phenotypic selection. Self‐pollinated offspring plants carrying HKT1;2 inheritance germination tolerance presence 100 m NaCl. Our work may pave way for transgene‐free alleles interest asexually sexually reproducing plants.

Language: Английский

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Application of CRISPR/Cas9-mediated gene editing for abiotic stress management in crop plants

Frontiers in Plant Science, Journal Year: 2023, Volume and Issue: 14

Published: April 18, 2023

Abiotic stresses, including drought, salinity, cold, heat, and heavy metals, extensively reducing global agricultural production. Traditional breeding approaches transgenic technology have been widely used to mitigate the risks of these environmental stresses. The discovery engineered nucleases as genetic scissors carry out precise manipulation in crop stress-responsive genes associated molecular network has paved way for sustainable management abiotic stress conditions. In this context, clustered regularly interspaced short palindromic repeat-Cas (CRISPR/Cas)-based gene-editing tool revolutionized due its simplicity, accessibility, adaptability, flexibility, wide applicability. This system great potential build up varieties with enhanced tolerance against review, we summarize latest findings on understanding mechanism response plants application CRISPR/Cas-mediated towards a multitude stresses metals. We provide mechanistic insights CRISPR/Cas9-based genome editing technology. also discuss applications evolving techniques such prime base editing, mutant library production, transgene free multiplexing rapidly deliver modern cultivars adapted

Language: Английский

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Orphan crops: their importance and the urgency of improvement

Planta, Journal Year: 2019, Volume and Issue: 250(3), P. 677 - 694

Published: June 12, 2019

Language: Английский

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What is the available evidence for the range of applications of genome-editing as a new tool for plant trait modification and the potential occurrence of associated off-target effects: a systematic map

Environmental Evidence, Journal Year: 2019, Volume and Issue: 8(1)

Published: July 12, 2019

Abstract Background Within the last decades, genome-editing techniques such as CRISPR/Cas, TALENs, Zinc-Finger Nucleases, Meganucleases, Oligonucleotide-Directed Mutagenesis and base editing have been developed enabling a precise modification of DNA sequences. Such provide options for simple, time-saving cost-effective applications compared to other breeding hence genome has already promoted wide range plant species. Although application induces less unintended modifications (off-targets) in classical mutagenesis techniques, off-target effects are prominent point criticism they supposed cause effects, e.g. genomic instability or cell death. To address these aspects, this map aims answer following question: What is available evidence new tool trait potential occurrence associated effects? This primary question will be considered by two secondary questions: One overview market-oriented traits being modified plants explores effects. Methods A literature search nine bibliographic databases, Google Scholar, 47 web pages companies governmental agencies was conducted using predefined tested strings English language. Articles were screened on title/abstract full text level relevance based pre-defined inclusion criteria. The relevant information included studies mapped data extraction strategy. Besides descriptive summary literature, spreadsheet containing all extracted provided. Results Altogether, 555 articles from journals, company identified 1328 studies/applications model agricultural crops period January 1996 May 2018. Most China followed USA. Genome-editing applied 68 different plants. most basic research, 99 28 leading with improved food feed quality, agronomic value like growth characteristics increased yield, tolerance biotic abiotic stress, herbicide industrial benefits. 252 explored CRISPR/Cas. Several firstly investigated whether sites show similarity target sequence secondly analyzed sequencing. In around 3% sites, mutations detected. Only few analyses unbiased detection methods (e.g. whole sequencing). No that could correlated process studies. Conclusions rapid adoption demonstrated considerable number market oriented (crops traits) described publications worldwide. Studies investigating very heterogeneous their structure design. Therefore, an in-depth assessment regarding weight mandatory.

Language: Английский

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Cassava breeding and agronomy in Asia: 50 years of history and future directions

Breeding Science, Journal Year: 2020, Volume and Issue: 70(2), P. 145 - 166

Published: Jan. 1, 2020

In Asia, cassava (Manihot esculenta) is cultivated by more than 8 million farmers, driving the rural economy of many countries. The International Center for Tropical Agriculture (CIAT), in partnership with national agricultural research institutes (NARIs), instigated breeding and agronomic 1983. program has successfully released high-yielding cultivars resulting an average yield increase from 13.0 t ha–1 1996 to 21.3 2016, significant economic benefits. Following success increasing yields, turned its focus higher-value traits, such as waxy cassava, reach new market niches. More recently, building resistance invasive pests diseases become a top priority due emergent threat mosaic disease (CMD). involves profitability advanced technologies focusing on better management practices thereby maintaining sustainable production systems. Remote sensing are being tested trait discovery large-scale field evaluation cassava. summary, Asia driven combination food demand technological innovations productivity. Further, exploration potential data-driven agriculture needed empower researchers producers advancement.

Language: Английский

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Creation of novel alleles of fragrance gene OsBADH2 in rice through CRISPR/Cas9 mediated gene editing

PLoS ONE, Journal Year: 2020, Volume and Issue: 15(8), P. e0237018 - e0237018

Published: Aug. 12, 2020

Fragrance in rice grains is a key quality trait determining its acceptability and marketability. Intensive research on aroma identified mutations betaine aldehyde dehydrogenase (OsBADH2) leading to production of rice. Gene editing technologies like CRISPR/Cas9 system has opened new avenues for accelerated improvement grain through targeted mutagenesis. In this study, we have employed tool create novel alleles OsBADH2 introduction into an elite non-aromatic variety ASD16. PCR analysis putative transformants using primers targeting the flanking regions sgRNA 7th exon 37.5% potential multi-allelic T0 generation. Sensory evaluation test leaves lines thirteen belonging five independent events producing aroma. Sequence these aromatic 22 different types located within -17 bp +15bp region. The -1/-2 deletion line # 8–19 -8/-5 2–16 produced strong phenotype was stably inherited T1 Comparative volatile profiling detected compounds viz., pyrrolidine, pyridine, pyrazine, pyradazine pyrozole progenies 8–19. This study demonstrated use creating introduce any varieties.

Language: Английский

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