Frequent variations and phylogenetic relationships within the genus Secale identified by ND-FISH according to the genome-wide universal oligonucleotides chromosome probes DOI Creative Commons
Zhi Li,

Zixin Sun,

Tianheng Ren

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 12, 2024

Introduction Rye ( Secale cereale L.) played a very important role in wheat genetic improvement and forage production worldwide. However, since rye is kind of cross-pollinated plant, high levels heterozygosity heterogeneity existed the genome. Genome-wide variation repeat sequences one most reasons for chromosome evolution rye. High-precision cytological identification can effectively identify heterochromatin or sequence variations genome, relationship between different varieties be identified while obtaining FISH-karyotype varieties. The chromosomes analyzed by degree probes on chromosomes. Methods All materials were non-denaturing fluorescence situ hybridization (ND-FISH). Five probes, (AAC) 6 , Oligo-pSc119.2-1, Oligo-pTa71A-2, Oligo-pSc200, Oligo-pSc250 used to Results 15 including S. (cultivated weedy rye), strictum (wild sylvestre vavilovii rye) examined five oligonucleotides probes. 92 signal sites 2074 patterns observed, suggesting that polymorphisms exist genomes. karyotypes obtained, frequency types at each site was calculated model diagrams Oligo-pSc200 + drawn. results showed rate not consistent. 1R, 6R, 7R have higher diversity, 2R 3R lower are more conserved relative other also indicated has far distance from species, might ancestors Chinese Discussion this study confirmed rapid change diversity

Language: Английский

Fine mapping of Pm71, a novel powdery mildew resistance gene from emmer wheat DOI Creative Commons
Jiadong Zhang, Hong Yang, Guohao Han

et al.

The Crop Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

2
Fine mapping of the powdery mildew resistance gene PmXQ-0508 in bread wheat DOI Creative Commons

Zejun Qian,

Guohao Han,

Ningning Yu

et al.

The Crop Journal, Journal Year: 2024, Volume and Issue: 12(4), P. 1176 - 1184

Published: Aug. 1, 2024

In a wheat breeding line XQ-0508 showing consistent resistance to powdery mildew disease, recessive gene, designated PmXQ-0508, was identified and mapped distal region on chromosome arm 2BS. Of three resistance-associated genes in this region, one encoding protein kinase selected as the primary candidate for PmXQ-0508. Ten closely linked DNA markers developed study could be used marker-assisted selection powdery-mildew programs.

Language: Английский

Citations

5
Genome-wide analysis of NAC transcription factors and exploration of candidate genes regulating selenium metabolism in Broussonetia papyrifera DOI

Longfei Guo,

Yongling Liao, Shiming Deng

et al.

Planta, Journal Year: 2024, Volume and Issue: 260(1)

Published: May 16, 2024

Language: Английский

Citations

4
Fine-mapping of PmHHM, a broad-spectrum allele from a wheat landrace conferring both seedling and adult resistance to powdery mildew DOI Creative Commons

Bisheng Fu,

Zhen Lin, Lijuan Yan

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Feb. 6, 2025

Common wheat is a leading global food crop that impacts security. Wheat powdery mildew (PM), caused by Blumeria graminis f. sp. tritici (Bgt), poses significant threat to grain yield and flour quality. The identification utilization of broad-spectrum resistance genes against PM are essential for effective disease control. spectrum test during the seedling stage adult were conducted evaluate landrace Honghuamai (HHM). Five segregating populations investigated assess inheritance in HHM. To map its resitance gene, bulked segregant analysis, molecular mapping comparative genomic analysis also used present study. HHM shows remarkable field nearly immune all 25 Bgt isolates tests, making it an excellent source resistance. was determined single dominant temporarily named PmHHM. It then fine-mapped interval with genetic distance 0.0031 cM physical 187.4 kb on chromosome 4AL Chinese Spring reference sequence v.2.1. Four identified target region, three which encode nucleotide-binding leucine-rich repeat (NLR) proteins. Comparative revealed presence/absence variations (PAVs) PmHHM locus among common varieties. These closely linked markers will not only benefit cloning gene underlying but facilitate efficient breeding programs.

Language: Английский

Citations

0
The wheat NLR pair RXL/Pm5e confers resistance to powdery mildew DOI Creative Commons
Guanghao Guo, Kaihong Bai,

Yikun Hou

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Summary Powdery mildew poses a significant threat to global wheat production and most cloned deployed resistance genes for breeding encode nucleotide‐binding leucine‐rich repeat (NLR) immune receptors. Although two genetically linked NLRs function together as an NLR pair have been reported in other species, this phenomenon has relatively less studied wheat. Here, we demonstrate that tightly genes, RXL Pm5e , arranged head‐to‐head orientation, mediate powdery The of the / is validated by mutagenesis, gene silencing, gene‐editing assays. Interestingly, both atypical NLRs, with possessing truncated NB‐ARC (nucleotide binding adaptor shared APAF‐1, plant R proteins CED‐4) domain featuring coiled‐coil (CC) domain. Notably, lack integrated associated effector recognition found all previously pairs. Additionally, exhibit preference forming hetero‐complexes rather than homo‐complexes, highlighting their cooperative role disease resistance. We further show CC specifically suppresses hypersensitive response induced through competitive interaction, revealing regulatory mechanisms within pair. Our study sheds light on molecular mechanism underlying Pm5e‐ mediated provides new example

Language: Английский

Citations

0
TaANK-TPR1 enhances wheat resistance against stripe rust via controlling gene expression and protein activity of NLR protein TaRPP13L1 DOI
Shuangyuan Guo, Feng Zhang, Xiaona Du

et al.

Developmental Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
An NLR receptor confers broad‐spectrum resistance to diversified powdery mildew sublineages in wheat and barley DOI Creative Commons

Renchun Fan,

Lei Feng, Yaling Liu

et al.

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: March 31, 2025

Language: Английский

Citations

0
Advancing wheat breeding using rye: a key contribution to wheat breeding history DOI Creative Commons

G C Han,

Hanwen Yan,

LI Li-hui

et al.

Trends in biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Genome-wide characterization of the NBLRR gene family provides evolutionary and functional insights into blast resistance in pearl millet (Cenchrus americanus (L.) Morrone) DOI

Aruljothi Ambalavanan,

Mallana Gowdra Mallikarjuna,

Shilpi Bansal

et al.

Planta, Journal Year: 2024, Volume and Issue: 259(6)

Published: May 4, 2024

Language: Английский

Citations

2
Bulked segregant RNA-seq reveals complex resistance expression profile to powdery mildew in wild emmer wheat W762 DOI Creative Commons

Zejun Qian,

Ruishan Liu,

Xueqing Liu

et al.

Frontiers in Plant Science, Journal Year: 2024, Volume and Issue: 15

Published: May 16, 2024

Powdery mildew, caused by Blumeria graminis f. sp. tritici ( Bgt ), is one of the most destructive fungal diseases threatening global wheat production. Exploring powdery mildew resistance Pm ) gene(s) and dissecting molecular mechanism host are critical to effectively reasonably control this disease. Durum Triticum turgidum L. var. durum Desf.) an important gene donor for improvement against mildew. In study, a resistant accession W762 was used investigate its potential component(s) profile expression pattern in responding invasion using bulked segregant RNA-Seq (BSR-Seq) further qRT-PCR verification. Genetic analysis showed that did not meet monogenic inheritance complex genetic model might exist within population × Langdon (susceptible wheat). After BSR-Seq, 6,196 consistently different single nucleotide polymorphisms (SNPs) were called between susceptible parents bulks, among them, 763 SNPs assigned chromosome arm 7B. Subsequently, 3,653 differentially expressed genes (DEGs) bulks annotated analyzed Gene Ontology (GO), Cluster Orthologous Groups (COG), Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment. The regulated selected their temporal patterns following inoculation. As result, nine disease-related distinctive after serve as targets regulate W762. Our study could lay foundation also provide durable

Language: Английский

Citations

1