Trans-Kingdom RNA Dialogues: miRNA and milRNA Networks as Biotechnological Tools for Sustainable Crop Defense and Pathogen Control DOI Creative Commons
Hui Jia, Pan Li, Mingcai Li

et al.

Plants, Journal Year: 2025, Volume and Issue: 14(8), P. 1250 - 1250

Published: April 20, 2025

MicroRNAs (miRNAs) are a class of non-coding RNAs approximately 20–24 nucleotides in length, which play crucial role during gene regulation plant–pathogen interaction. They negatively regulate the expression target genes, primarily at transcriptional or post-transcriptional level, through complementary base pairing with sequences. Recent studies reveal that pathogen infection, miRNAs produced by plants and miRNA-like (milRNAs) fungi can endogenous genes their respective organisms undergo trans-kingdom transfer. thereby recipient cells. These findings provide novel perspectives for deepening our understanding regulatory mechanisms underlying interactions. Here, we summarize discuss roles milRNAs mediating interactions via multiple pathways, providing new insights into functions these modes action. Collectively, lay theoretical foundation targeted management crop diseases.

Language: Английский

The molecular dynamics between reactive oxygen species (ROS), reactive nitrogen species (RNS) and phytohormones in plant’s response to biotic stress DOI

Kiran Gogoi,

Hiren Gogoi,

Manashi Borgohain

et al.

Plant Cell Reports, Journal Year: 2024, Volume and Issue: 43(11)

Published: Oct. 16, 2024

Language: Английский

Citations

11
Eukaryotic translation initiation factor OseIF5A4 negatively regulates rice immunity against blast and bacterial blight diseases DOI Creative Commons
Hui Wang, Yuqing Yan, Yan Bi

et al.

The Crop Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

1
A novel key virulence factor, FoSSP71, inhibits plant immunity and promotes pathogenesis in Fusarium oxysporum f. sp. cubense DOI Creative Commons
Shuang Liu, Junyu Wu,

Yinhui Sun

et al.

Microbiology Spectrum, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

ABSTRACT Fusarium wilt of banana ( Musa spp.), caused by oxysporum f. sp. cubense Foc ), poses a significant threat to the global industry. Particularly, tropical race 4 exhibits high pathogenicity toward major commercial cultivar Cavendish, and there are no effective control measures available. Here, we characterize novel effector protein, FoSSP71, from , which was significantly induced during early stages interaction could suppress BAX-triggered programmed cell death in Nicotiana benthamiana . Transient expression FoSSP71 N. leaves weaken upregulation genes involved SA signaling pathway flg22 reduce both reactive oxygen species bursts callose accumulation. To verify function deletion mutant created. The displayed reduced growth rate F. marked reduction virulence bananas compared wild type (WT). Furthermore, levels PR3 PR10 were downregulated infected with ΔFoSSP71 strain WT strain. These findings indicate that is essential for plays key role invasion. Therefore, presents potential target future control, offering scientific foundation breeding disease-resistant varieties developing measures. IMPORTANCE Effector proteins critical factors fungi, playing roles fungal infection plants. In this study, identified an important regulatory protein invasion 4). Understanding its mechanisms necessary. Our research indicates factor 4, as it suppresses plant immune responses inhibiting accumulation callose. lacking showed bananas, demonstrating controlling disease. provide basis resistant disease new strategies, crucial sustainable development

Language: Английский

Citations

1
Plant secondary metabolites-mediated plant defense against bacteria and fungi pathogens DOI
Wenjuan Yang, Lu Zhang, Yong Yang

et al.

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 217, P. 109224 - 109224

Published: Oct. 20, 2024

Language: Английский

Citations

7
Phytoalexin gene regulation in Arabidopsis thaliana – On the verge of a paradigm shift? DOI Creative Commons
Ivan Monsalvo,

Jie Lin,

Nik Kovinich

et al.

Current Plant Biology, Journal Year: 2024, Volume and Issue: 39, P. 100367 - 100367

Published: June 30, 2024

Phytoalexins are plant-specialized metabolites that biosynthesized de novo in response to pathogens. Their biosynthesis is highly diverse, with different plant lineages biosynthesizing unique molecules. A common notion individual species produce one type of phytoalexin molecule. Arabidopsis well known an indole alkaloid phytoalexin, named camalexin, as important component its defense against microbial However, studies collectively demonstrate produces a diverse array molecules from branches primary and specialized metabolism. The signaling proteins stimulate synthesis conserved plants, yet each transcription factor they converge upon has been reported regulate the specific class metabolite. We have conducted this review because recent demonstrated homologs factors dissimilar biosynthetic pathways other species. These findings challenge paradigm role regulating Here, we Arabidopsis, them, discoveries on their regulatory mechanisms. discuss crop suggest WRKY33, ERF1, ANAC042, MYB15, MYB72, protein JAZ1, part 'core' network conserved, regulates distinct lineages. Finally, highlight questions raised by that, once solved, will provide major advances our understanding evolution biochemical defenses plants. Recent evidence demonstrates these can be manipulated enhance production pathogen resistance

Language: Английский

Citations

4
Virulence Regulation in Plant-Pathogenic Bacteria by Host-Secreted Signals DOI Creative Commons
Muhammad Asif, Xin Xie, Zhibo Zhao

et al.

Microbiological Research, Journal Year: 2024, Volume and Issue: 288, P. 127883 - 127883

Published: Aug. 24, 2024

Bacterial pathogens manipulate host signaling pathways and evade defenses using effector molecules, coordinating their deployment to ensure successful infection. However, host-derived metabolites as signals, critical role in regulating bacterial virulence requires further insights. Effective regulation of virulence, which is essential for pathogenic bacteria, involves controlling factors that enable colonization, defense evasion, tissue damage. This dynamic, influenced by environmental cues including signals from plants like exudates. Plant exudates, comprising diverse compounds released roots tissues, serve rich chemical affecting the behavior associated bacteria. nutrients act molecules are sensed through membrane-localized receptors intracellular response mechanisms review explains how different bacteria detect answer secreted gene expression. Our main emphasis exploring recognition process host-originated molecular sensors on cellular membranes pathways. encompasses insights into strains individually coordinate various distinct can positively or negatively regulate virulence. Furthermore, we explained interruption plant with perception dampen pathogen The intricate interplay between particularly recognize metabolic genes, portrays a crucial initial interaction leading profound influences infection outcomes. work will greatly aid researchers developing new strategies preventing treating infections.

Language: Английский

Citations

4
Detecting novel plant pathogen threats to food system security by integrating the Plant Reactome and remote sensing DOI
Seth C. Murray, Aart Verhoef, Alper Adak

et al.

Current Opinion in Plant Biology, Journal Year: 2025, Volume and Issue: 83, P. 102684 - 102684

Published: Jan. 5, 2025

Language: Английский

Citations

0
OsWRKY26 negatively regulates bacterial blight resistance by suppressing OsXa39 expression DOI Creative Commons
Win Tun, Kieu Thi Xuan Vo, Behnam Derakhshani

et al.

Frontiers in Plant Science, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 9, 2025

Plants are susceptible to infection by various pathogens with high epidemic potential. Xanthomonas oryzae pv. (Xoo) causes bacterial blight in rice, one of the most significant diseases both temperate and tropical regions. In this study, we report identification characterization OsWRKY26, a sucrose-inducible transcription factor, that plays role plant defense responses following Xoo infection. We found mutant plants defective OsWRKY26 showed enhanced response specifically Xoo, indicating factor acts as negative regulator. contrast, did not exhibit higher resistance compared wild-type (WT) when infected rice blast fungal pathogen Magnaporthe oryzae. Transcriptomic analysis WT revealed several genes were upregulated mutants. Of these, selected OsXa39 for further analysis. Transient expression experiments protoplasts repressed Luciferase reporter gene driven promoter. Chromatin immunoprecipitation binds directly promoter region OsXa39. These findings suggest negatively regulates during repressing well other pathogen-related such OsXa47, OsBBR1, OsRSR1, OsPR1a, OsPR1-11, OsPR2, OsPR4c.

Language: Английский

Citations

0
The 14-3-3 protein CaTFT7 interacts with transcription factor CaHDZ27 to positively regulate pepper immunity against Ralstonia solanacearum DOI Creative Commons
Shaoliang Mou, Xiaodan Chen,

Jiao Cai

et al.

Horticulture Research, Journal Year: 2025, Volume and Issue: 12(4)

Published: Jan. 14, 2025

Abstract Bacterial wilt, caused by Ralstonia solanacearum, is a devastating disease affecting plants in the Solanaceae family. In our previous study, CaHDZ27 was shown to act crucially pepper defense response R. solanacearum. However, molecular basis underlying function remains unexplored. this we demonstrate that post-translationally regulated 14-3-3 protein CaTFT7, which functions as positive regulator immunity against RT-qPCR analysis revealed CaTFT7 transcriptionally induced solanacearum infection. The data from virus-induced gene silencing positively affects immunity, further confirmed of CaTFT7-overexpressing Nicotiana benthamiana. interacted with CaHDZ27, thereby promoting stability and enhancing binding promoter cysteine-rich receptor-like kinase 5 (CaCRK5), above indicated enhanced promoted its ability activate shedding light on mechanisms resistance bacterial wilt.

Language: Английский

Citations

0
Atypical RXLR effectors are involved in Phytophthora cactorum pathogenesis DOI Creative Commons

Zeyang Zheng,

Juanjuan Liu,

Wenzhong You

et al.

aBIOTECH, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

RXLR effectors are pathogenic factors secreted from oomycetes to manipulate the immunity of host. Typical contain an RXLR-dEER motif at N-terminus, whereas atypical RXLRs show variations on this motif. The oomycete Phytophthora cactorum is known infect over 200 plant species, resulting in significant agricultural economic losses. Although genome-wide identification and functional analyses typical P. have been performed, little PcaRXLRs. Here, we identified RXLRs, both atypical, compared them with those other pathogens. Fewer were possibly due fewer duplication events RXLRs. In contrast, percentage was higher than suggesting roles pathogenesis. Analysis gene expression showed that most transcribed, their functionality. Transient two Nicotiana benthamiana they induced necrosis dependent host SGT1 HSP90. Furthermore, additional suppressed defense response N. promoted infection. These results demonstrate vital role provide valuable information evolutionary patterns interactions plants. online version contains supplementary material available 10.1007/s42994-025-00198-4.

Language: Английский

Citations

0