FRUIT GROWING RESEARCH,
Journal Year:
2024,
Volume and Issue:
40, P. 57 - 62
Published: Dec. 11, 2024
The
preservation
and
use
of
the
genetic
resources
is
basis
for
permanent
improvement
cultivated
varieties.
objective
this
study
was
to
analyze
quality
local
Romanian
apples
cultivars
as
parents
in
future
apple
breeding
programs.
experiment
conducted
during
2020-2022
collection
Genetic
Breeding
Department
Research
Institute
Fruit
Growing
Pitești,
Romania.
designed
assess
fruits
on
fifteen
('Botane',
'Călugăresc',
'Citron
de
Somcuța',
'Cormose',
'Crețesc',
'Crețesc
Simici',
'Domnesc',
'Muntenești',
'Mohorât',
'Moți',
'Sătmărești',
'Urdoase',
'Verzisoare',
'Zmeurii').
As
control
cultivar,
we
used
'Jonathan'.
Quality
parameters
investigated
were
weight,
caliber,
total
soluble
solids,
firmness
color.
results
showed
that
highest
values
fruit
found
'Verzisoare'
variety
(83.03
HPE
units),
solids
(°Brix)
'Urdoase'
(20.63°Brix).
Maximum
size
has
been
registered
Simici'
(186.67
g)
also
caliber
(85.4
mm)
reached
at
same
cultivar.
ABSTRACT
Flower
color
is
a
crucial
trait
that
attracts
pollinators
and
determines
the
ornamental
value
of
floral
crops.
Cymbidium
lowianum
,
one
most
important
breeding
parent
hybrids,
has
two
flower
morphs
(normal
albino)
differ
in
lip
color.
However,
molecular
mechanisms
underlying
formation
C.
are
not
well
understood.
In
this
study,
comparative
metabolomic
analysis
between
normal
albino
tissues
indicated
cyanidin‐3‐
O
‐glucoside
content
was
significantly
higher
red
epichiles
than
other
tissues.
This
finding
suggests
responsible
for
variation
differentiation
.
We
also
found
coloration
correlated
with
high
levels
F3'H
expression;
further,
anthocyanins,
carotenoids
chlorophyll
coordinate
to
influence
sepal
petal
during
development.
transgenic
Arabidopsis
lines,
overexpression
increased
anthocyanin
concentration,
BCH
carotenoid
whereas
HEMG
CHLI
both
concentration.
Identification
assessment
several
transcription
factors
revealed
MYB308‐1
activates
MYB111
PIF4‐2
activate
expression
Importantly,
MYB14‐1
shows
interaction
PIF4‐2,
appears
act
as
connector
biosynthesis
by
either
activating
or
inhibiting
expression.
These
results
indicate
that,
patterns
primarily
regulated
types
concentrations
flavonoids;
The
evolutionary
origin
of
fruits
played
a
pivotal
role
in
promoting
the
dominance
angiosperms
on
Earth
as
protect
and
nourish
seeds
facilitate
their
dispersal
through
diverse
mechanisms.
Understanding
molecular
networks
underlying
fruit
development
is
prerequisite
for
elucidating
mechanisms
shaping
diversification,
particularly
improving
crop
yield
quality
response
to
rapid
climate
change
modern
agricultural
systems.
In
this
article,
we
offer
comprehensive
analysis
classification,
emphasising
intrinsic
characteristics
adaptive
strategies
specific
environments.
Based
studies
model
systems
such
Arabidopsis
thaliana
Solanum
lycopersicum,
highlight
recent
advances
identifying
novel
components
involved
development.
We
further
discuss
that
contribute
diversification
context
well-established
phylogenetic
frameworks,
with
examples
from
Brassicaceae
Solanaceae
families.
A
comparison
between
indicates
key
module
largely
conserved
evolution.
propose
future
research
integrates
multidisciplinary
evidence
could
help
better
understand
which
ultimately
practice.
Journal of Experimental Botany,
Journal Year:
2024,
Volume and Issue:
75(18), P. 5627 - 5640
Published: May 29, 2024
Strawberry
(Fragaria
×
ananassa)
is
a
model
plant
for
studying
non-climacteric
fruit
ripening
regulated
by
abscisic
acid
(ABA);
however,
the
signaling
of
ABA
in
regulation
coloration
not
fully
understood.
In
this
study,
we
identified
transcription
factor
BASIC
HELIX-LOOP-HELIX
3
(bHLH3)
as
being
key
to
via
yeast
two-hybrid
library
screening
using
bait
SUCROSE
NONFERMENTING
1
(SNF1)-RELATED
PROTEIN
KINASE
2
(SnRK2.6),
which
core
component
that
negatively
regulates
ripening.
The
interaction
was
also
confirmed
firefly
luciferase
complementation
assays
and
pull-down
assays.
RT-qPCR
western
blot
analysis
bHLH3
expressed
ubiquitously
strawberry
tissues,
it
stably
during
development.
Overexpression
RNAi
both
SnRK2.6
demonstrated
promote
inhibit
coloration,
respectively.
Using
EMSAs,
showed
promotes
expression
UDP-GLUCOSE:
FLAVONOL-O-GLUCOSYLTRANSFERASE
(UFGT),
gene
anthocyanin
biosynthesis,
directly
binding
its
promoter.
We
determined
can
phosphorylate
inhibits
UFGT
promoter,
consequently
suppressing
expression.
Altogether,
propose
increased
content
leads
decreased
SnRK2.6,
turn
releases
phosphorylation
thereby
enhances
expression,
ultimately
promoting
fruit.
Molecular Horticulture,
Journal Year:
2024,
Volume and Issue:
4(1)
Published: July 1, 2024
Abstract
The
color
of
red-skinned
pear
(
Pyrus
spp.)
is
primarily
attributed
to
accumulation
anthocyanins,
which
provide
nutritional
benefits
for
human
health
and
are
closely
associated
with
the
commercial
value
fruits.
Here,
we
reported
functional
characterization
a
R2R3-MYB
repressor
PyMYB107,
forms
an
‘activator-repressor’
loop
control
anthocyanin
in
pear.
PyMYB107
overexpression
inhibited
biosynthesis
both
calli
fruits,
while
virus-induced
gene
silencing
increased
Furthermore,
ectopic
expression
decreased
tomato,
strawberry
tobacco.
can
competitively
bind
PybHLH3
PyMYB10/MYB114,
thereby
suppressing
transcriptional
activation
key
genes,
PyANS
PyUFGT
.
Site-directed
mutagenesis
showed
that
mutations
within
R3
domain
EAR
motif
eliminated
its
repressive
activity.
Additionally,
exhibited
comparable
pattern
PyMYB10
/
MYB114
was
transcriptionally
activated
by
them.
Our
finding
advanced
comprehension
repression
mechanism
underlying
accumulation,
providing
valuable
molecular
insights
into
improving
quality
Plant Cell & Environment,
Journal Year:
2024,
Volume and Issue:
47(12), P. 4833 - 4848
Published: Aug. 5, 2024
Abstract
Peach
varieties
that
differ
in
red
coloration
due
to
varied
anthocyanin
accumulation
result
from
transcriptional
regulation
by
PpMYB10s
,
a
group
of
specific
R2R3
MYBs.
Here
we
investigated
the
mechanisms
driving
lack
yellow‐skinned
‘Jinxiu’
peach
peel,
as
well
induced
UV
irradiance.
It
was
found
PpMYB10.1
PpMYB10.2
and
PpMYB10.3
were
positive
regulators
accumulation,
but
stimulation
weak.
Low
expression
causes
natural
deficiency
peel.
However,
promoter
sequences
identical
naturally
red‐coloured
‘Hujingmilu’.
Therefore,
potential
negative
regulator(s)
upstream
explored.
A
novel
R2R3‐MYB
repressor
termed
PpMYB80
identified
through
comparative
transcriptomic
analysis
then
functionally
confirmed
via
transiently
overexpressing
silencing
fruit,
transformation
tobacco.
directly
binds
inhibits
its
expression,
does
not
affect
.
In
UV‐exposed
upregulated,
while
remained
low
enhanced,
which
results
This
study
revealed
cascade
involving
PpMYB
activators
repressors
regulating
basal
UV‐induced
Frontiers in Plant Science,
Journal Year:
2025,
Volume and Issue:
15
Published: Jan. 13, 2025
The
genetic
basis
of
type
1
red-flesh
color
development
in
apple
(
Malus
domestica
)
depends
upon
a
particular
allele
the
MdMYB10
gene.
Interestingly,
apples
are
fully
red
after
fruit
set,
but
anthocyanin
pigmentation
cortex
may
decrease
during
growth
and
maturation,
leading
to
variable
patterning
intensities
mature
cortical
flesh.
We
developed
histogram-based
analysis
method
quantitatively
estimate
patterns.
This
methodology
was
applied
investigate
phenotypic
diversity
four
hybrid
F1
families
segregating
for
color.
Pigmentation
patterns
were
found
be
heritable
allowing
identification
new
locus
by
QTL
analysis.
To
further
mechanisms
involved
spatial
deposition
anthocyanin,
metabolome,
transcriptome
methylome
comparisons
between
white
flesh
areas
within
genotype
cv.
‘R201’
exhibiting
patterns,
performed.
Wide-targeted
showed
that
white-flesh
accumulate
more
dihydrochalcones
hydroxycinnamic
acids
than
while
flavonoids.
Anthocyanin
biosynthesis
genes
positive
regulators
(MBW
complex)
up-regulated
areas,
reduction
storage,
transport
stability
(increase
pH,
down-regulation
MdGSTU22
an
increase
phenolic
catabolism
concomitant
with
fading
process
areas.
Expression
linked
differentially
methylated
region
(DMR)
suggesting
potential
environmental
effect
on
epigenetic
control
gene
expression
fading.
Altogether,
these
results
provide
first
characterization
functional
