Transcriptomic and Metabolomic Analysis Reveals the Mechanism of H18 Pepper Color Change

Agriculture, Journal Year: 2025, Volume and Issue: 15(6), P. 655 - 655

Published: March 20, 2025

Pigments in plants not only determine their visual characteristics but also play crucial roles photosynthesis, pollinator attraction, and disease resistance. The diverse colors of chili peppers arise from various pigments. However, detailed reports on the genetic metabolic changes these pigments developmental stages colorful are limited. In this study, we analyzed content anthocyanins carotenoids fruits cultivated pepper variety H18 at different growth development stages. Our findings revealed that, as mature, anthocyanin gradually decreases while carotenoid increases. Using transcriptomic metabolomic sequencing stages, identified four types anthocyanins: pelargonidin, cyanidin, delphinidin, peonidin, well a small amount proanthocyanidins. concentrations generally showed decreasing trend, expression patterns biosynthesis genes were consistent with data. analysis carotenoids, found apart Lutein, all other detected increased during fruit development. Most began to accumulate significantly orange stage (H18-O) peaked red (H18-R). Transcriptomic RT-qPCR data indicated that progressively. Furthermore, investigated degradation potential genes, including BGLU, POD, PPO. This study provides deep insights into mechanisms underlying pigment peppers, which may contribute improvement varieties future research regulatory mechanisms.

Language: Английский

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Genome‐Wide Identification and Functional Characterization of QdA3oGT Genes Reveal a Molecular Mechanism of Autumnal Leaf Color Transition in Quercus dentata

Plant Cell & Environment, Journal Year: 2025, Volume and Issue: unknown

Published: April 15, 2025

ABSTRACT Autumnal leaf color transition is a crucial environmental adaptation strategy for trees. Quercus dentata typical autumn tree species distributed in Northeast Asia, which characterized by bright red leaves autumn. However, the molecular mechanisms of turning respond to climatic factors are unclear. This study identified five anthocyanidin 3‐ O ‐glucosyltransferase ( QdA3oGT ) members, QdA3oGT2 , with unique cis ‐elements, evolutionary background and gene structure, emerged as key transition. The transcript level was significantly higher than other members. qPCR revealed that expression trend increased coloration, positively correlated anthocyanin content. Thus, Q. . Functional validation Arabidopsis demonstrated stimulated low temperature, leading enhanced cold resistance accumulation. Furthermore, cold‐responsive senescence‐related transcription factor, QdNAC58 identified, directly bound NAC conserved binding motif ‘ACGTCA’ promoter, regulated In sum, this reveals QdNAC58, responds temperatures autumn, further upregulates play role synthesis associated senescence.

Language: Английский

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Tracing the color: quantitative trait loci analysis reveals new insights into red-flesh pigmentation in apple (Malus domestica)

Horticulture Research, Journal Year: 2024, Volume and Issue: 11(8)

Published: June 27, 2024

Abstract Red-flesh color development in apple fruit is known to depend upon a particular allele of the MdMYB10 gene. While anthocyanin metabolic pathway well characterized, current genetic models do not explain observed variations red-flesh pigmentation intensity. Previous studies focused on total content as phenotypic trait characterize overall flesh color. this approach led global understanding mechanisms involved expression, it essential adopt more quantitative approach, by analyzing other phenolic compound classes, order better understand molecular subtle variation and distribution. In study, we performed pedigree-based loci (QTL) mapping, using FlexQTL™ software, decipher determinism five F1 inter-connected families segregating for trait. A 452 genotypes were evaluated profiles during 3 years (2021–2023). We identified 24 QTLs intensity profiles. Six detected LG1, LG2, LG8, LG9, LG11, LG16. Several genes QTL confidence intervals related metabolism. Further analyses allowed us propose model which competition between anthocyanins flavan-3-ols (monomer oligomer) end-products decisive development. model, alleles favorable high can be inherited from both white-flesh parents.

Language: Английский

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Thermopriming Induces Time-Limited Tolerance to Salt Stress

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(14), P. 7698 - 7698

Published: July 13, 2024

Implementing sustainable crop protection practices is crucial to protect global harvests and ensure high-quality food supplies. While priming an established method in seed production for the fortification of plants against various stresses, it not yet a standard practice transplant cultivation. Thus, we evaluated long-term effects thermopriming—a heat-based technique—on growth, development, fruit yield tomato plants. Following recovery period about six weeks thermoprimed without stress inducers, subjected them subsequent salt ascertain persistence effects. Additionally, compared efficacy thermopriming with benzothiadiazole (BTH), chemical elicitor, enhancing plant resilience abiotic stress. BTH application negatively impacted both growth health, showed no such adverse on these parameters. Instead, initially enhanced defense mechanisms by increasing accumulation protective phenols flavonoids leaves. Interestingly, while did alter response stress, notably strengthened overall Our findings underscore potential temporal constraints memory. Nonetheless, primed exhibited temporarily increased tolerance, offering means safeguard offspring.

Language: Английский

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Unravelling the physiological and molecular mechanisms 0f leaf color change in Acer griseum through multi-omics analysis

Plant Physiology and Biochemistry, Journal Year: 2024, Volume and Issue: 216, P. 109198 - 109198

Published: Oct. 16, 2024

Language: Английский

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Metabolomic and transcriptomic analyses provide insights into temperature and light regulated anthocyanin accumulation in flesh of ‘Furongli’ plum (Prunus salicina Lindl.)

Postharvest Biology and Technology, Journal Year: 2024, Volume and Issue: 221, P. 113326 - 113326

Published: Nov. 27, 2024

Language: Английский

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Tissue-specific transcriptome analyses unveils candidate genes for flavonoid biosynthesis, regulation and transport in the medicinal plant Ilex asprella

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Dec. 2, 2024

It is not clear that the genes involved with flavonoids synthesis, regulation and transport in Ilex asprella. Transcriptome analysis of leaf, stem root has uncovered 28,478 differentially expressed (DEGs) are various biological processes. Among these, expression 31 candidate synthetase genes, 19 transcription factors, 5 transporters associated flavonoid biosynthesis varies across tissues, encompassing seven complete biosynthetic pathways (stilbene, aurone, flavone, isoflavone, flavonol, phlobaphene, anthocyanin) one partial pathway (proanthocyanidin). Tissue-specific patterns suggest stilbenes, aurones, flavones anthocyanin branches more prominent roots, as indicated by key such STS(Ilex_044726), CH4ʹGT(Ilex_047989), FNS(Ilex_043640) UFGT(Ilex_014720). In leaves, phlobaphenes flavonols dominant, determined CHI(Ilex_005941), FNR(Ilex_039777) FLS(Ilex_046424). The isoflavone appears to be active stems due presence IFS(Ilex_029360), mirroring accumulation intermediate metabolite chalcone, which regulated CHS(Ilex_047537). absence LAR implies gallocatechin, catechin liked proanthocyanidins cannot synthesized I. Meanwhile, general phenylpropanoid than evidenced PAL(Ilex_042231, Ilex_014816), C4H(Ilex_017598), 4CL(Ilex_042033). Flavanone, dihydroflavonol leucoanthocyanidin, intermediates, accumulate rapidly stem, root, respectively, F3H(Ilex_004635) DFR(Ilex_004771). Correlation network analyses reveal regulators closely synthesis genes. study provides profound snoop into metabolism asprella offers valuable refer for medicinal plant.

Language: Английский

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Bioactive constituents from Carica papaya fruit: implications for drug discovery and pharmacological applications

Applied Biological Chemistry, Journal Year: 2024, Volume and Issue: 67(1)

Published: Dec. 12, 2024

Abstract Carica papaya , commonly known as papaya, is a fruit recognized for its substantial medicinal potential, primarily due to wide range of bioactive compounds. This review thoroughly examines the pharmacological implications these constituents and highlights their potential applications in drug discovery therapy. Papaya abundant vitamins (A, C, E), minerals, enzymes, phytochemicals such flavonoids, phenolic acids, carotenoids, alkaloids, all which contribute antioxidant, anti-aging, anti-inflammatory, anticancer effects. The demonstrates significant activity against diabetes, obesity, cardiovascular diseases, gastrointestinal disorders. also discusses how environmental factors, including temperature, light, soil quality, rainfall, impact phytochemical composition thereby influencing properties. Both vitro vivo studies have highlighted therapeutic papaya-derived compounds various health conditions, cancer, wound healing, health. Additionally, we explore papaya's role promoting gut antimicrobial properties bacterial viral pathogens. In conclusion, diverse activities position it valuable candidate further research development applications.

Language: Английский

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Effect of Preharvest Treatments with Sodium Bicarbonate and Potassium Silicate in Navel and Valencia Oranges to Control Fungal Decay and Maintain Quality Traits during Cold Storage

Agronomy, Journal Year: 2023, Volume and Issue: 13(12), P. 2925 - 2925

Published: Nov. 28, 2023

The quality of sweet orange (Citrus sinensis L.) is determined by the presence decay caused phytopathogenic fungi. This can develop in field and rapidly spread among oranges during postharvest storage. Currently, conventional treatments applied to control this problem are chemical fungicides. However, consumers demand eco-friendly non-polluting alternatives with low residues. Therefore, aim work preharvest application sodium bicarbonate (SB) potassium silicate (PS) solutions at 0.1 1% Navel Valencia elucidate effect on fruit fungal harvest after 42 days storage 8 °C. Results showed that treated SB 0.1%, PS 0.1, maintained traits similar levels ones. reduced firmness increased weight loss, respiration rate, maturity index, citrus color index. total carotenoid content significantly 1%, no differences were observed other compared control. Total antioxidant activity phenolic decreased contrary results PS, where both parameters increased. Regarding decay, best obtained highest doses PS. use sprays could be an alternative without affecting quality.

Language: Английский

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Relationship of Cell Size Distribution and Biomechanics of Strawberry Fruit Under Varying Ca and N Supply

Food and Bioprocess Technology, Journal Year: 2024, Volume and Issue: unknown

Published: July 20, 2024

Abstract Bruising due to compression of strawberry fruit is the major cause for decay, resulting in food waste, which can be observed macroscopically as result texture failures. In present study, laser light scattering density (LSD) analysis was applied study effect cell size distribution percentages on mechanics, considering three growth stages and added variance by foliar spray application Ca, N, Ca-N. The rate k range 300–450 µm enhanced N compared Ca treatment with 0.8 0.5, respectively. Comparison sizes mechanical tissue properties reveals a strong mechanics. Based data, particles discrete element models (DEM) were established, applying simple contact model Hertz-Mindlin test LSD data input, when simulating peak force tissue. Comparing measured DEM simulation, mean square error reduced, e.g., group over-ripe fruit, from 9.6 6.5% considered. Concluding, newly available provide valuable information enable simulation under varying environmental conditions. accuracy LSD-DEM approach makes relevant plant nutrition, developing robot harvesters, sorting devices, shelf life assessment fresh fruit.

Language: Английский

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