Advances in Plant Metabolomics and Its Applications in Stress and Single-Cell Biology

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(13), P. 6985 - 6985

Published: June 23, 2022

In the past two decades, post-genomic era envisaged high-throughput technologies, resulting in more species with available genome sequences. In-depth multi-omics approaches have evolved to integrate cellular processes at various levels into a systems biology knowledge base. Metabolomics plays crucial role molecular networking bridge gaps between genotypes and phenotypes. However, greater complexity of metabolites diverse chemical physical properties has limited advances plant metabolomics. For several years, applications liquid/gas chromatography (LC/GC)-mass spectrometry (MS) nuclear magnetic resonance (NMR) been constantly developed. Recently, ion mobility (IMS)-MS shown utility resolving isomeric isobaric metabolites. Both MS NMR combined metabolomics significantly increased identification quantification an untargeted targeted manner. Thus, hyphenated tools will narrow gap number metabolite features identified Metabolites change response environmental conditions, including biotic abiotic stress factors. The spatial distribution across different organs, tissues, cells compartments is trending research area Herein, we review recent technological advancements their understanding organization. addition, discuss opportunities challenges multiple interactions, multi-omics, single-cell

Language: Английский

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Transcriptome profile analysis of Indian mustard (Brassica juncea L.) during seed germination reveals the drought stress-induced genes associated with energy, hormone, and phenylpropanoid pathways

Plant Physiology and Biochemistry, Journal Year: 2023, Volume and Issue: 200, P. 107750 - 107750

Published: May 15, 2023

Language: Английский

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Seasonal drought promotes citrate accumulation in citrus fruit through the CsABF3‐activated CsAN1‐CsPH8 pathway

New Phytologist, Journal Year: 2024, Volume and Issue: 242(3), P. 1131 - 1145

Published: March 14, 2024

Summary Plenty of rainfall but unevenly seasonal distribution happens regularly in southern China. Seasonal drought from summer to early autumn leads citrus fruit acidification, how regulates citrate accumulation remains unknown. Herein, we employed a set physiological, biochemical, and molecular approaches reveal that CsABF3 responds stress modulates fruits by directly regulating CsAN1 CsPH8 . Here, demonstrated irreversible acidification is caused lasting for > 30 d during the enlargement stage. We investigated transcriptome characteristics affected corroborated pivotal roles bHLH transcription factor ( ) P 3A ‐ATPase gene response drought. Abscisic acid (ABA)‐responsive element binding 3 was upregulated an ABA‐dependent manner. activated expression specifically ABA‐responsive elements (ABREs) promoters positively regulated accumulation. Taken together, this study sheds new light on regulatory module ABA‐CsABF3‐ ‐ responsible under stress, which advances our understanding quality formation fruit.

Language: Английский

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Understanding drought tolerance in plants

Physiologia Plantarum, Journal Year: 2021, Volume and Issue: 172(2), P. 286 - 288

Published: May 27, 2021

Drought limits plant growth and productivity, the severity duration of drought determine its damaging effects. Almost all phenological stages are prone to drought, especially germination flowering. Global climate change is exacerbating effects drought. Understanding mechanisms underpinning responses challenging due variations in traits controlling changing water availability soil demand for at evaporation sites, status, genetic differences among species. affects relations, nutrient acquisition assimilation, photosynthesis, enzyme functioning assimilates partitioning (Xiong et al. 2020; Ayyaz al., 2021; Bano 2021). The visible morphological contributing altered relations use efficiency include reduced leaf number, size, stem extension root 2020). often characterised by reductions cell content, turgor tissue potential, resulting wilting, stomatal closure enlargement. These lead photosynthetic arrest, triggering drastic changes normal metabolism that can cessation or death. Reductions hydraulic conductivity shoots roots under result from interruptions xylem column (embolism) modifications vessels. Leaf regulated aquaporin transport proteins (Patel Mishra tolerance involves metabolic readjustment cellular whole level, combined with finely signalling events. Several key physiological adaptations response xero-halophytic plants have been identified (Panda Physiological, biochemical molecular level triggered counteract maintain performance. a complex process genes. upregulates genes, such as those involved osmolyte metabolism, secondary metabolite synthesis hormone synthesis, which facilitate rapid induction crosstalk. Reducing loss increasing diffusive resistance, developing prolific deep system enhanced uptake, small succulent leaves transpirational important strategies (Osakabe 2014; van der Vyver Peters 2017). Mineral ions like potassium assist osmotic adjustment, thereby maintenance while silicon improves retention capacity through silicification endodermal cells. Low weight osmolytes crucial sustaining drought-induced injury protecting metabolism. Among hormones, auxins, gibberellic acid, ABA, salicylic jasmonic acid ethylene contribute modulating response. Polyamines metabolites exhibit antioxidant reducing adverse Phytohormones, Abscisic (ABA), act regulators conditions, pathways either ABA-dependent ABA-independent. ABA mediated increased Water (WUE) regulation has reported Gouveia (2020). largely weak ROS scavenging excessive damage macromolecules, proteins, lipids nucleic acids, hampers organelles, mitochondria chloroplasts. ROS-induced peroxidation polyunsaturated fatty acids causes ion leakage, leaving cells deficient mineral Loss homeostasis directly pathways, disturbing structural functional profile plant. system, compatible help minimise Molecular studies shown above-mentioned machinery other stresses. components, non-enzymatic ascorbate, glutathione tocopherol, an essential role maintaining redox processes electron transport. Modern research techniques further strengthen our understanding these mechanisms. gene sets local systematic sensing need be model crops (e.g. Arabidopsis) before extending crops. advancement genome sequencing, assembly annotation improved genomics related crop plants. Genome-wide association (GWAS), used studying natural variations, identify genes alleles regulation, will pave way mapping cloning loci regulating controlled hormonal seed development relations. In addition current management practices, fertiliser application compost (Hafez 2020), exogenous biostimulants (Nadeem 2020) phytohormones (Khan tested their efficacy improving mitigation oxidative approaches physio-biochemical evaluations revealed several avenues plants, food discussed researchers (Jabeen Razik Aleem omics approaches, transcriptomics, metabolomics proteomics, drought-responsive including coding channels, transporters, detoxification, late embryogenesis abundant proteolysis. Signal transduction expression during stress transcription factors (TFs), phosphatases protein kinases, mitogen-activated, Ca-dependent kinases. TFs AREB, NAC, AP2/ERF, MYB, bZIP MYC. Some factor families AP2-EFR, Dof-type, MADS-box, bZIP, CPP, ZF-HD GATA-type drought-stressed cowpea (Ferreira-Netoet Transcriptomic study discussing identification pepper Negi Of note dehydration-responsive element binding (DREB) CRT AP2/ERF family, well-studied structure stabilisation, DNA post-translational modification transgenic studies. carried (2020) demonstrated significant difference patterns between sensitive tolerant cultivars. Other induced desiccation encoding osmo-protectants, proline, glycine betaine, sugars biosynthetic pathway, components TFs. regulate downstream stress-responsive cis-regulatory elements promoter region However, needed understand physiology, biochemistry genetics This special issue covers advances experimental evidence confirming roles novel molecules, hydrogen sulphide, melatonin acid. Transgenic using microRNAs candidate understating performance conditions also discussed. We hope this provides excellent set papers greater ideas future on biology.

Language: Английский

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Negative effects of long‐term exposure to salinity, drought, and combined stresses on halophyte Halogeton glomeratus

Physiologia Plantarum, Journal Year: 2021, Volume and Issue: 173(4), P. 2307 - 2322

Published: Oct. 9, 2021

Plants are subjected to salt and drought stresses concurrently but our knowledge about the effects of combined stress on plants is limited, especially halophytes. We aim study if some diverse tolerance traits in halophyte may explain their salinity stresses, individual combination, identify key that influence growth under such conditions. Here, Halogeton glomeratus was grown control, single or combinations 60 days treatments, morphophysiological responses were tested. Our results showed drought, salinity, combination these two stressors decreased plant (shoot height, root length, biomass), leaf photosynthetic pigments content (chlorophyll a, b, a + b carotenoids), gas exchange parameters (Net photosynthesis rate [PN ], transpiration [E], stomatal conductance [gs ]), water potential (ψw ), decreases more prominent treatment compared with individually performed. Similarly, induced severe oxidative as indicated by hydrogen peroxide (H2 O2 ) malondialdehyde (MDA) accumulated. Nevertheless, H. equipped specific mechanisms protect itself against including upregulation superoxide dismutases (SOD; EC 1.15.1.1) catalase (CAT; 1.11.1.6) activities accumulation osmoprotectants (Na+ , Cl- soluble sugar). content, parameters, potential, APX activity, CAT sugar, H2 MDA valuable screening criteria for salt, alone combined, provide tolerant assessment glomeratus.

Language: Английский

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ROS scavenging and ion homeostasis is required for the adaptation of halophyte Karelinia caspia to high salinity

Frontiers in Plant Science, Journal Year: 2022, Volume and Issue: 13

Published: Oct. 3, 2022

The halophyte Karelinia caspia has not only fodder and medical value but also can remediate saline-alkali soils. Our previous study showed that salt-secreting by salt glands is one of main adaptive strategies K. under high salinity. However, ROS scavenging, ion homeostasis, photosynthetic characteristics responses to salinity remain unclear in caspia. Here, physio-biochemical gene expression associated with scavenging ions transport were tested subjected 100-400 mM NaCl for 7 days. Results both antioxidant enzymes (SOD, APX) activities non-enzymatic antioxidants (chlorogenic acid, α-tocopherol, flavonoids, polyamines) contents significantly enhanced, accompanied up-regulating the related enzyme synthesis (KcCu/Zn-SOD, KcAPX6, KcHCT, KcHPT1, Kcγ-TMT, KcF3H, KcSAMS KcSMS) increasing concentrations NaCl. These are beneficial removing excess maintain a stable level H2O2 O2 - without lipid peroxidation response salt. Meanwhile, KcSOS1/2/3, KcNHX1, KcAVP was linked Na+ compartmentalization into vacuoles or excretion through Notably, improve function PSII facilitate net rates, which helpful growing normally saline. Overall, findings suggested systems Na+/K+ synergistically contributed redox equilibrium, enhancement function, thereby conferring tolerance.

Language: Английский

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Metabolic profiles in the xylem sap of Brassica juncea exposed to cadmium

Physiologia Plantarum, Journal Year: 2023, Volume and Issue: 175(2)

Published: March 1, 2023

Abstract Metabolic profiles in xylem sap are considered a fundamental mechanism for Cadmium (Cd) detoxification plants. However, the metabolic of Brassica juncea response to Cd is still unclear. Here, we investigated effects on metabolomics B. treated with at different times by utilizing nontargeted liquid chromatography‐mass spectrometry (LC‐MS)‐based method further elucidating exposure. The findings indicated that 48 h and 7 days exposure caused significant differences sap. Those differential metabolites primarily involved amino acids, organic lipids, carbohydrates, most them were downregulated, which played essential roles stress. Furthermore, resisted 48‐h via regulation glycerophospholipid metabolism, carbon aminoacyl‐tRNA biosynthesis, glyoxylate dicarboxylate linoleic acid C5‐branched dibasic alpha‐linolenic cyanoamino ABC transporters, biosynthesis pyrimidine metabolism; whereas photosynthesis, oxidative phosphorylation regulated resisting 7‐day

Language: Английский

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Hexadecanoic acid produced in the co-culture of S. cerevisiae and E.coli promotes oxidative stress tolerance of the S.cerevisiae cells

World Journal of Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 40(7)

Published: May 24, 2024

Language: Английский

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Transcriptome and Metabolome Analyses Reveal the Molecular Mechanisms of Albizia odoratissima’s Response to Drought Stress

Plants, Journal Year: 2024, Volume and Issue: 13(19), P. 2732 - 2732

Published: Sept. 29, 2024

Albizia odoratissima is a deciduous tree species belonging to the family Leguminosae. It widely distributed in southern subtropical and tropical areas of China has important ecological economic value. The growth metabolic processes A. are affected by drought stress, but molecular mechanisms remain unknown. Therefore, this study investigated physicochemical properties, gene expression, metabolites seedlings under stress. results show that, leaves seedlings, stress reduced moisture content, chlorophyll photosynthetic efficiency, superoxide dismutase (SOD) activity, gibberellin (GA) indoleacetic acid (IAA) contents while increasing catalase (CAT) peroxidase (POD) activities malondialdehyde (MDA), proline, soluble sugar, protein contents. Within CK5 (Day 5 control group) vs. T5 treatment), CK10 T10, CK15 T15, CK20 T20 groups (CK: group; T: total 676 differentially expressed genes (DEGs) were upregulated 518 DEGs downregulated, 228 143 differential accumulation (DAMs) identified T10 groups. These mainly involved amino alkaloid metabolism pathways seedlings. In biosynthesis pathways, relative expression levels AoproA (Aod04G002740, ORTHODONTIC APPLIANCE), AoOAT (Aod07G015970, ORNITHINE-OXO-ACID TRANSAMINASE), AoAOC3 (Aod12G005010/08G003360/05G023920/08G003000/08G003010, AMINE OXIDASE COPPER CONTAINING 3) increased, which concurrently promoted arginine, piperine, cadaverine, lysine. Furthermore, some key transcription factors response using weighted co-expression network analyses (WGCNA) method. findings reveal that respond improving capacities antioxidant system secondary metabolism.

Language: Английский

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Ectopic expression of NAC transcription factor HaNAC3 from Haloxylon ammodendron increased abiotic stress resistance in tobacco

Planta, Journal Year: 2022, Volume and Issue: 256(6)

Published: Oct. 31, 2022

Language: Английский

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