A dynamic basal complex modulates mammalian sperm movement

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: June 21, 2021

Abstract Reproductive success depends on efficient sperm movement driven by axonemal dynein-mediated microtubule sliding. Models predict sliding at the base of tail – centriole but such has never been observed. Centrioles are ancient organelles with a conserved architecture; their rigidity is thought to restrict Here, we show that, in mammalian sperm, atypical distal (DC) and its surrounding pericentriolar matrix form dynamic basal complex (DBC) that facilitates cascade internal deformations, coupling beating asymmetric head kinking. During beating, DC’s right side surroundings slide ~300 nm rostrally relative left side. The deformation throughout DBC transmitted head-tail junction; thus, tilts left, generating kinking motion. These findings suggest evolved as linker into single self-coordinated system.

Language: Английский

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Sperm bauplan and function and underlying processes of sperm formation and selection

Physiological Reviews, Journal Year: 2021, Volume and Issue: 102(1), P. 7 - 60

Published: April 21, 2021

The spermatozoon is a highly differentiated and polarized cell, with two main structures: the head, containing haploid nucleus acrosomal exocytotic granule, flagellum, which generates energy propels cell; both structures are connected by neck. sperm’s aim to participate in fertilization, thus activating development. Despite this common bauplan function, there an enormous diversity structure performance of sperm cells. For example, mammalian spermatozoa may exhibit several head patterns overall lengths ranging from ∼30 350 µm. Mechanisms transport female tract, preparation for recognition interaction oocyte also show considerable variation. There has been much interest understanding origin diversity, evolutionary terms relation mechanisms underlying differentiation testis. Here, relationships between function examined at levels: first, analyzing selective forces that drive changes physiology understand adaptive values variation impact on male reproductive success second, examining cellular molecular formation testis explain how can give rise such wide array forms functions.

Language: Английский

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Mysteries and unsolved problems of mammalian fertilization and related topics

Biology of Reproduction, Journal Year: 2022, Volume and Issue: 106(4), P. 644 - 675

Published: March 15, 2022

Mammalian fertilization is a fascinating process that leads to the formation of new individual. Eggs and sperm are complex cells must meet at appropriate time position within female reproductive tract for successful fertilization. I have been studying various aspects mammalian over 60 years. In this review, discuss many different fertilization, some my laboratory's contribution field, enigmas mysteries remain be solved.

Language: Английский

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The evolution of centriole degradation in mouse sperm

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 2, 2024

Abstract Centrioles are subcellular organelles found at the cilia base with an evolutionarily conserved structure and a shock absorber-like function. In sperm, centrioles flagellum essential for embryo development in basal animals. Yet, sperm have evolved diverse forms, sometimes acting like transmission system, as cattle, becoming dispensable, house mice. How centriole to become dispensable some organisms is unclear. Here, we test hypothesis that this transition occurred through cascade of evolutionary changes proteins, structure, function was possibly driven by competition. We final steps associated change primary centriolar inner scaffold protein FAM161A rodents. This information provides first insight into molecular mechanisms adaptive evolution underlying major within internal mammalian neck.

Language: Английский

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Sperm Differentiation: The Role of Trafficking of Proteins

International Journal of Molecular Sciences, Journal Year: 2020, Volume and Issue: 21(10), P. 3702 - 3702

Published: May 24, 2020

Sperm differentiation encompasses a complex sequence of morphological changes that takes place in the seminiferous epithelium. In this process, haploid round spermatids undergo substantial structural and functional alterations, resulting highly polarized sperm. Hallmark during process include formation new organelles, chromatin condensation nuclear shaping, elimination residual cytoplasm, assembly sperm flagella. To achieve these transformations, have unique mechanisms for protein trafficking operate coordinated fashion. Microtubules filaments actin are main tracks used to facilitate transport mechanisms, assisted by motor non-motor proteins, delivery vesicular non-vesicular cargos specific sites. This review integrates recent findings regarding role differentiation. Although complete characterization interactome proteins involved temporal spatial processes is not yet known, we propose model based on current literature as framework future investigations.

Language: Английский

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Energy Metabolism and Hyperactivation of Spermatozoa from Three Mouse Species under Capacitating Conditions

Cells, Journal Year: 2022, Volume and Issue: 11(2), P. 220 - 220

Published: Jan. 10, 2022

Mammalian sperm differ widely in morphology, and several explanations have been presented to account for this diversity. Less is known about variation physiology cellular processes that can give cells an advantage when competing fertilize oocytes. Capacitation of spermatozoa, a process essential mammalian fertilization, correlates with changes motility result characteristic swimming pattern as hyperactivation. Previous studies revealed velocity depend on the amount ATP available and, therefore, movement occurring during capacitation hyperactivation may involve bioenergetics. Here, we examine differences levels from three mouse species (genus

Language: Английский

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Bioenergetics of Rodent Spermatozoa

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown, P. 267 - 288

Published: Jan. 1, 2025

Language: Английский

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Evolutionary Aspects of Sperm Physiology and Its Assessment

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown, P. 29 - 41

Published: Jan. 1, 2025

Language: Английский

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Modeling diffusive search by non-adaptive sperm: Empirical and computational insights

PLoS Computational Biology, Journal Year: 2025, Volume and Issue: 21(4), P. e1012865 - e1012865

Published: April 17, 2025

During fertilization, mammalian sperm undergo a winnowing selection process that reduces the candidate pool of potential fertilizers from ~10 6 -10 11 cells to 10 1 2 (depending on species). Classical competition theory addresses positive or ‘stabilizing’ acting phenotypes within populations organisms but does not strictly address developmental consequences traits among individual are under purifying during fertilization. It is latter utmost concern for improving assisted reproductive technologies (ART) because low-fitness may be inadvertently used fertilization interventions rely heavily artificial selection, such as intracytoplasmic injection (ICSI). Importantly, some form in nearly all forms ART (e.g., differential centrifugation, swim-up, hyaluronan binding assays, etc.). To date, there no unifying quantitative framework (i.e., selection) synthesizes causal mechanisms with observed natural variation traits. In this report, we reframe physiological function collective diffusive search and develop multi-scale computational models explore dynamics constrain fitness Several experimentally useful concepts developed, including probabilistic measure well an information theoretic magnitude each which assessed systematic increases microenvironmental selective pressure motility patterns.

Language: Английский

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Assessments of sperm quality integrating morphology, swimming patterns, bioenergetics and cell signalling

Theriogenology, Journal Year: 2020, Volume and Issue: 150, P. 388 - 395

Published: Feb. 11, 2020

Language: Английский

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