Comparison of Development and Antioxidative Ability in Fertilized Crossbred (Yorkshire × Landrace × Duroc) Oocytes Using Duroc and Landrace Sperm DOI Creative Commons
Hayoung Lee, Hye Won Kim, Jisoon An

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(24), P. 3562 - 3562

Published: Dec. 10, 2024

Pig production through crossbreeding methods is a pillar of the swine industry; however, research on fertilization ability male pigs in crossbreeds lacking. Therefore, this study investigated effects Duroc sperm (DS) and Landrace (LS) fertility Yorkshire × (YLD) oocytes. Sperm were collected from species, characteristics, viability, acrosome reactions analyzed using flow cytometry. Oocytes YLD ovaries, DS LS was determined vitro (IVF). Reactive oxygen species (ROS) antioxidative abilities H2DCFDA Cell Tracker Red assay. Pluripotency (OCT4, SOX2, NANOG), (SOD1, SOD2, CAT, GPx1), apoptotic (Bax Bcl-2), cell cycle-related (Cdc2 CCNB1) genes detected quantitative reverse transcription polymerase chain reaction (qRT-PCR) oocytes fertilized with sperm. The results showed no significant difference viability or between LS. ROS levels significantly lower group than group, whereas glutathione (GSH) embryo did not differ groups. OCT4, GPx1, Cdc2 mRNA expression higher Blastocyst formation reduced, blastocyte increased LS-obtained embryos. In conclusion, these provide fundamental understanding semen three-way pigs.

Language: Английский

Assessment of Boar Semen Morphology and Morphometry DOI
J. Gadea

Methods in molecular biology, Journal Year: 2025, Volume and Issue: unknown, P. 43 - 61

Published: Jan. 1, 2025

Language: Английский

Citations

0
Application of sperm motion kinematics and motility-related proteins for prediction of male fertility DOI
Jeong-Won Bae, Ju-Mi Hwang,

Woo-Jin Lee

et al.

Theriogenology, Journal Year: 2024, Volume and Issue: 218, P. 223 - 230

Published: Feb. 7, 2024

Language: Английский

Citations

2
Effect of age, season, breed composition, and sperm counting chamber on boar semen quality in tropics DOI Open Access
Francisco Sevilla, Laura Murillo, Ignacio Araya‐Zúñiga

et al.

Published: Aug. 21, 2024

Understanding the factors affecting semen quality is important for optimizing male reproductive efficiency. The present study aimed to evaluate seminal associated with breed, age, season, and sperm counting chamber in boar ejaculates. A total of 22 sexually mature healthy boars from maternal sire commercial breeds were utilized as donors, an average age (mean ± standard deviation) 21.0 7.2 months. Boars housed individually well-ventilated pens fed a breeder mix. Semen samples collected each dry rainy season. grouped according into three categories: <12 months, 12-24 >24 Only ejaculates at least 75 % morphologically normal more than 8.5 x 109 per ejaculate used. analyzed using Computer-Assisted Analysis (CASA) ISAS®v1 system. Results showed that younger (<12 months) exhibited higher progressive motility faster swimming patterns compared older boars. percentages fast spermatozoa increased by kinematics variables significant differences (P<0.05) between chambers. Duroc Landrace presented greater motility, while terminal line accelerated linear progressiveness (P<0.05). Overall, impact various on kinematic ejaculates, including genetic breed composition, enables better understand This highlights importance swine management practices active

Language: Английский

Citations

2
Morphometry of Boar Spermatozoa in Semen Stored at 17 °C—The Influence of the Staining Technique DOI Creative Commons

Dorota Szablicka,

Anna Wysokińska, Angelika Pawlak

et al.

Animals, Journal Year: 2022, Volume and Issue: 12(15), P. 1888 - 1888

Published: July 25, 2022

The aim of the study was to assess morphometry sperm during storage liquid boar semen at 17 °C. An attempt also made evaluate suitability three staining methods for assessment morphometry. carried out on 20 Landrace boars. Semen collected from boars every 5 days by manual method. Four ejaculates each were analysed (80 in total). Analyses performed five times: 1 h, 24 48 96 and 168 h after collection. Blisters with insemination doses opened immediately before analyses. From dose, smears prepared morphometric evaluation sperm, which stained (eosin-nigrosin-EN, eosin-gentian-EG, SpermBlue-SB). Morphometric measurements 15 randomly selected normal morphology slide. included following parameters: head length, width, area, perimeter; tail length; total length. results used calculate shape index. dimensions shown change extent these changes, however, depended method used, as result different case both tail. In slides eosin-nigrosin method, smaller time than SpermBlue eosin-gentian methods.

Language: Английский

Citations

6
Effect of an Antioxidant Supplement Combination on Boar Sperm DOI Creative Commons
Ivan Galić, S. Dragin, Ivan Stančić

et al.

Animals, Journal Year: 2022, Volume and Issue: 12(10), P. 1301 - 1301

Published: May 18, 2022

The study was conducted on a commercial pig farm located in Serbia. Thirty Duroc or Landrace breed boars were randomly selected for this study. experimental group fed compound feed with added organic selenium and Oxynat 3D. antioxidant status parameters of boar seminal plasma evaluated using biochemical analyzer Randox kits. sperm chromatin structure assay (SCSA) flow cytometry (FC) provided information about spermatozoa's DNA status. Additionally, the total number motile spermatozoa kinematic measured computer-assisted analysis (CASA) system. aim to improve semen by combining two preparations that have potential effect, but also establish level various enzymes native sperm. There no statistically significant difference capacity glutathione peroxidase activity obtained from control groups boars. Regarding superoxide dismutase activity, research results showed compared one. Moreover, fragmentation lower higher values, respectively, groups. combination these shows significantly enhanced vital semen. To best our knowledge, is first which ejaculate examined after application supplements.

Language: Английский

Citations

4
Influence of Fat-Soluble Vitamin Intramuscular Supplementation on Kinematic and Morphometric Sperm Parameters of Boar Ejaculates DOI Creative Commons
Josué Calderón‐Calderón, Francisco Sevilla, Eduardo R. S. Roldán

et al.

Frontiers in Veterinary Science, Journal Year: 2022, Volume and Issue: 9

Published: July 12, 2022

Ejaculate quality can be regarded as multifactorial, with nutrition being a factor that could directly influence sperm parameters. The present study aimed to evaluate seminal associated seasonal fat-soluble vitamin supplementation of boars. Seven sexually mature boars were randomly allotted one the three groups, and fed supplementary diets for 32 weeks: (1) control treatment (COD), without vitamins, (2) containing 100% administered intramuscularly, which was based on fat soluble (A, D3, E) (FVD1), (3) 50% (FVD 12 ). Semen collected at 7-day intervals. samples analyzed assess several parameters using Computer-Assisted Analysis (CASA) ISAS ® v1 system. Results showed groups receiving FVD1 FVD id="M2">12 had an increased semen volume. percentages motile progressively by treatment. A statistically significant interaction between season found in percentage motility progressive ( p < 0.05). Sperm concentrations differences 0.05) treatments. Velocity variables (VSL, VCL, VAP) higher received comparison controls no supplementation. presented spermatozoa greater head size more elongated heads Overall, utilization dietary significantly improved boar ejaculates. This highlights importance active

Language: Английский

Citations

4
El análisis seminal en la agricultura de precisión en el siglo XXI DOI Creative Commons
Carles Soler, Anthony Valverde

Agronomía Mesoamericana, Journal Year: 2023, Volume and Issue: unknown, P. 51957 - 51957

Published: Jan. 30, 2023

Introducción. La reproducción asistida de animales tiene su origen en la domesticación las especies ganaderas útiles al ser humano. Los consiguientes procesos selección artificial permitieron desarrollar técnicas biotecnológicas que contribuyeron a mejora capacidades producción animal. Objetivo. Contextualizar el análisis seminal agricultura precisión siglo XXI. Desarrollo. visualización espermatozoides microscopio puede considerarse como primer paso advenimiento biotecnología y base para desarrollo asistida. Con perfeccionamiento los métodos microscopía, se logró caracterizar gametos masculinos, lo significó un avance significativo tecnología inseminación artificial. El punto inflexión marcado por conservación espermática, implicó cambio sustantivo estas tecnologías ganaderas, ya sea con semen crio preservado o refrigerado. Estos son alto valor caso amenazadas, dado pueden crear bancos germoplasma propósitos genética rescate riesgo extinción. ha desarrollado forma paralela asistida, hoy considera una técnica relevante animal, perfeccionado mediante ciencia tecnología, física óptica computación. Conclusión. tenido paradigma desechar obsoletas evaluación subjetiva calidad adoptar objetivos del semen, grandes volúmenes datos variables movilidad, cinéticas, morfométricas, morfológicas fragmentación ADN, permiten mejor eyaculados reproductores centros

Citations

2
Morphometric assessment of cryopreserved livestock bull spermatozoa in the tropics DOI
Luis Víquez, Francisco Sevilla, Ignacio Araya‐Zúñiga

et al.

Reproduction in Domestic Animals, Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 21, 2023

Abstract The identification of different morphometric patterns spermatozoa serves as a basis for improving our understanding the diversity in an ejaculate and to relate them potential fertility males. In this study, we aimed examine semen subpopulation structure, following dilution extenders, using mathematical approach possible application analyses. Ten sexually mature Bos taurus bulls were randomly allotted one three groups: (1) Tris–citric acid–egg yolk extender (Tris‐EY); (2) commercial egg OptiXcell® (3) Triladyl®. results showed significant differences ( p < .05) between extenders terms values head size shape variables individual sperm, indicating influence composition. Sperm width was found significantly differ according extender, decreasing order: OptiXcell ® (4.836 ± 0.017 μm), Triladyl (4.695 0.012 μm) Tris‐EY (4.638 0.010 μm). Principal component analysis allowed us identify two subpopulations , each Tris‐EY. Overall, observed sperm within .05), with bovine species that can be related functionality capabilities.

Language: Английский

Citations

1
Importance and variability of the paternal component in sow reproductive traits DOI Creative Commons
G. Cieleń, Ewa Sell‐Kubiak

Journal of Applied Genetics, Journal Year: 2024, Volume and Issue: 65(4), P. 853 - 866

Published: Oct. 18, 2024

Abstract Reproductive traits are an integral part of the goals breeding programs that contribute to economic success production. phenotypes such as litter size, number piglets born alive, or weight at birth mainly attributed females. Thus, maternal components can be found by default in quantitative genetics’ animal models. Still, paternal contribution variance should not discarded. In this review, we indicate importance effects pig describing both biology and genetics boars’ traits, use (non-)genetic service sire genetic models for measured on females, genes involved male reproduction. We start important biological boars have most effect their reproductive abilities, i.e., sexual maturity, sperm quality, testes parameters. Then move possible environmental could affect those (e.g., feed, temperature). The main review detail describes (i.e., heritability) direct females correlations). then non-genetic estimated percentage total which vary depending breed from 1 4.5% 2%, respectively. Finally, focus description candidate confirmed mutations affecting reproduction success: IGF2 , Tgm8 ESR1 ZSWIM7 ELMO1. conclusion, observed female might come various attributes including aspects. Those neglected they traits.

Language: Английский

Citations

0
Comparison of Development and Antioxidative Ability in Fertilized Crossbred (Yorkshire × Landrace × Duroc) Oocytes Using Duroc and Landrace Sperm DOI Creative Commons
Hayoung Lee, Hye Won Kim, Jisoon An

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(24), P. 3562 - 3562

Published: Dec. 10, 2024

Pig production through crossbreeding methods is a pillar of the swine industry; however, research on fertilization ability male pigs in crossbreeds lacking. Therefore, this study investigated effects Duroc sperm (DS) and Landrace (LS) fertility Yorkshire × (YLD) oocytes. Sperm were collected from species, characteristics, viability, acrosome reactions analyzed using flow cytometry. Oocytes YLD ovaries, DS LS was determined vitro (IVF). Reactive oxygen species (ROS) antioxidative abilities H2DCFDA Cell Tracker Red assay. Pluripotency (OCT4, SOX2, NANOG), (SOD1, SOD2, CAT, GPx1), apoptotic (Bax Bcl-2), cell cycle-related (Cdc2 CCNB1) genes detected quantitative reverse transcription polymerase chain reaction (qRT-PCR) oocytes fertilized with sperm. The results showed no significant difference viability or between LS. ROS levels significantly lower group than group, whereas glutathione (GSH) embryo did not differ groups. OCT4, GPx1, Cdc2 mRNA expression higher Blastocyst formation reduced, blastocyte increased LS-obtained embryos. In conclusion, these provide fundamental understanding semen three-way pigs.

Language: Английский

Citations

0