Morphometric assessment of cryopreserved livestock bull spermatozoa in the tropics DOI
Luis Víquez, Francisco Sevilla, Ignacio Araya‐Zúñiga

et al.

Reproduction in Domestic Animals, Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 21, 2023

Abstract The identification of different morphometric patterns spermatozoa serves as a basis for improving our understanding the diversity in an ejaculate and to relate them potential fertility males. In this study, we aimed examine semen subpopulation structure, following dilution extenders, using mathematical approach possible application analyses. Ten sexually mature Bos taurus bulls were randomly allotted one three groups: (1) Tris–citric acid–egg yolk extender (Tris‐EY); (2) commercial egg OptiXcell® (3) Triladyl®. results showed significant differences ( p < .05) between extenders terms values head size shape variables individual sperm, indicating influence composition. Sperm width was found significantly differ according extender, decreasing order: OptiXcell ® (4.836 ± 0.017 μm), Triladyl (4.695 0.012 μm) Tris‐EY (4.638 0.010 μm). Principal component analysis allowed us identify two subpopulations , each Tris‐EY. Overall, observed sperm within .05), with bovine species that can be related functionality capabilities.

Language: Английский

Efecto del diluyente, la edad y el estado sexual bovino sobre la cinemática del semen DOI Creative Commons
Ignacio Araya‐Zúñiga, Francisco Sevilla, Vinicio Barquero

et al.

Agronomía Mesoamericana, Journal Year: 2023, Volume and Issue: unknown, P. 52597 - 52597

Published: July 12, 2023

Introducción. Los patrones cinemáticos del semen se condicionan por variación individual y/o un efecto ambiental determinado el manejo posterior a la obtención de muestra. Objetivo. Evaluar dos diluyentes comerciales, edad y estado sexual los toros sobre movilidad cinética espermática fresco bovino. Materiales métodos. El estudio realizó en las fincas ganaderas “La Vega”, San Carlos, Balsa”, Ramón, Costa Rica, entre marzo agosto 2021. Se utilizó proveniente trece Brahman, extraído electroeyaculación. utilizaron 45 eyaculados total, con mínimo máximo cinco animal. diluyó Optixcell® Bioxcell®, una temperatura 37 °C. Las muestras analizaron mediante Sistema Computarizado Análisis Seminal (Computer-Assisted Semen Analysis, CASA)-Mot ISAS®v1 cámara recuento reutilizable Spermtrack® 20 μm profundidad. Resultados. observaron diferencias (p<0,05) respecto cinemática espermatozoides, así como parámetros espemática, según diluyente utilizado. Con uso Bioxcell® hubo mayor progresiva (64,03±2,01 %) comparación Optixcell®. diluidos presentaron patrón cinemático más lineal progresivo que Optixcell®, valores menores velocidad promedio (VAP=106,67±0,24 s-1) e índice rectitud (STR=79,35±0,10 %). La no mostró para sin embargo, fue animales >48 meses (62,27±1,87 observó sexual, durante monta presentó linealidad, oscilación comparado al descanso sexual. Conclusión. edad, utilizado preservación condicionaron calidad bovinos.

Citations

3
Kinematic and Morphometric Assessment of Fresh Semen, before, during and after Mating Period in Brahman Bulls DOI Creative Commons
Ignacio Araya‐Zúñiga, Francisco Sevilla,

Rafael Molina-Montero

et al.

Animals, Journal Year: 2023, Volume and Issue: 14(1), P. 132 - 132

Published: Dec. 29, 2023

The objective of the present study was to determine effects that reproductive season has on motility, kinematics, morphology, and sperm morphometry Brahman bulls evaluated with a commercial CASA system. experiment carried out at Costa Rica Institute Technology from March August 2021. A total eight were used. 28 ejaculates collected in pre-mating period (PMP), during it (DMP), after (AMP) using an electroejaculator. concentration measured Accuread photometer. motility Spermtrack® counting chamber. analyses performed CASA-Mot ISAS®v1 morphology analyzed microscope negative phase contrast objective. Morphometry CASA-Morph. did not differences between PMP AMP; however, significantly higher than DMP (p > 0.05). Regarding progressiveness variables, linearity forward progression (LIN), straightness (STR), wobble (WOB) < 0.05) DMP. kinematic principal component analysis grouped all variables into three factors effect found parameters head middle part sperm, such as width perimeter, which greater PMP. length show differences; both larger AMP. insertion distance piece Finally, contained cells angle These findings are important understand implications status quality consider them andrological evaluations.

Language: Английский

Citations

2
Evaluating the effect of semen storage and dilution rate on boar sperm quality DOI
José Manuel León Jurado, Francisco Sevilla, Ignacio Araya‐Zúñiga

et al.

Acta Agriculturae Scandinavica Section A – Animal Science, Journal Year: 2024, Volume and Issue: unknown, P. 1 - 13

Published: Nov. 27, 2024

This study analyzed the effect of boar semen storage and dilution rate on sperm motility, swimming patterns kinematics. It used 35 sexually mature, healthy boars, with an average age 19.0 ± 10.34 months known fertility. Samples were separated into four treatments according to ratio (1:1, 1:4, 1:8 1:16; v:v) Androstar Plus® refrigerated at 17°C in dark for 96 h. Subsamples 12, 24, 28, 72 h using ISAS®v1 Computer-Assisted Semen Analysis System (CASA). Total progressive motility decreased (P < 0.05) as time increased. The percentage morphological abnormalities increased after 48 storage. diluted 1:1 (v:v) had higher values total faster spermatozoa compared other rates. was concluded that plasma membrane integrity showed a significant downward pattern

Language: Английский

Citations

0
Morphometric assessment of cryopreserved livestock bull spermatozoa in the tropics DOI
Luis Víquez, Francisco Sevilla, Ignacio Araya‐Zúñiga

et al.

Reproduction in Domestic Animals, Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 21, 2023

Abstract The identification of different morphometric patterns spermatozoa serves as a basis for improving our understanding the diversity in an ejaculate and to relate them potential fertility males. In this study, we aimed examine semen subpopulation structure, following dilution extenders, using mathematical approach possible application analyses. Ten sexually mature Bos taurus bulls were randomly allotted one three groups: (1) Tris–citric acid–egg yolk extender (Tris‐EY); (2) commercial egg OptiXcell® (3) Triladyl®. results showed significant differences ( p < .05) between extenders terms values head size shape variables individual sperm, indicating influence composition. Sperm width was found significantly differ according extender, decreasing order: OptiXcell ® (4.836 ± 0.017 μm), Triladyl (4.695 0.012 μm) Tris‐EY (4.638 0.010 μm). Principal component analysis allowed us identify two subpopulations , each Tris‐EY. Overall, observed sperm within .05), with bovine species that can be related functionality capabilities.

Language: Английский

Citations

1