Unveiling Urinary Biomarkers, Crucial Genes, and Immune Infiltration in Lupus Nephritis Patients DOI Creative Commons

Huiqiong Zeng,

Zhongyu Xie, Haofei Hu

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 17, 2023

Abstract Background This study was conducted to investigate the expression patterns and biological roles of urinary long non-coding RNAs (lncRNAs) messenger (mRNAs) in individuals diagnosed with lupus nephritis (LN). Methods The cohort comprised six participants: three systemic erythematosus (SLE) involving LN, SLE without healthy controls (CON). Microarray technology employed analyze mRNAs lncRNAs, thereby exploring alterations overall RNA expression. Functional insights into dysregulated differentially expressed (DEMs) associated LN were derived through gene ontology (GO) enrichment analysis, Kyoto Encyclopedia Genes Genomes (KEGG) pathway set analysis (GSEA). Furthermore, construction a protein-protein interaction (PPI) network accomplished using Search Tool for Retrieval Interacting Genes/Proteins (STRING). identification immune-related cell types facilitated by Single-sample Gene Set Enrichment Analysis (ssGSEA). To predict potential drug candidates based on genes (DEGs), Connectivity Map (CMap) conducted. Results Within urine samples patients, total 247 602 lncRNAs exhibited differential relative control group. Among these, 83 down-regulated 141 up-regulated DEMs specifically discerned patients LN. GO highlighted processes such as transcriptional regulation, intrinsic apoptotic signaling pathways response DNA damage, regulation mitophagy. KEGG primarily revealed protein processing within endoplasmic reticulum, apoptosis, P53 pathway. Co-expression PPI suggested that nodes higher degrees connectivity concentrated related apoptosis autophagy. An assessment immune infiltration unveiled correlation between activated B cells CD56 dim natural killer (NK) pathogenesis. prediction drugs implicated inhibition mechanistic Aurora kinase A ( AURKA ) primary targeted intervention. molecular docking process confirmed robust binding activity hub genes' components. Conclusion has illuminated distinct profiles (LN) patients. These profiles, particularly context autophagy, involvement, provide valuable LN's underlying mechanisms. therapeutic targets, , offers promising directions future interventions management.

Language: Английский

Glucose metabolism is controlled by non-coding RNAs in autoimmune diseases; a glimpse into immune system dysregulation DOI
Raed Obaid Saleh,

Raed Fanoukh Aboqader Al-Aouadi,

Nujud Almuzaini

et al.

Human Immunology, Journal Year: 2025, Volume and Issue: 86(3), P. 111269 - 111269

Published: Feb. 24, 2025

Language: Английский

Citations

1
Advances in RNA therapy for the treatment of autoimmune diseases DOI
Ying Zhang, Chenyang Zang, Manyun Mao

et al.

Autoimmunity Reviews, Journal Year: 2025, Volume and Issue: unknown, P. 103753 - 103753

Published: Jan. 1, 2025

Language: Английский

Citations

0
Exosomes derived let-7f-5p is a potential biomarker of SLE with anti-inflammatory function DOI Creative Commons
Yi‐Jing Liu,

Hai-bing Miao,

Shu Lin

et al.

Non-coding RNA Research, Journal Year: 2025, Volume and Issue: 12, P. 116 - 131

Published: Feb. 22, 2025

Language: Английский

Citations

0
Biological functions and affected signaling pathways by Long Non-Coding RNAs in the immune system DOI Creative Commons

Hossein Ghahramani Almanghadim,

Bahareh Karimi,

Sepehr Valizadeh

et al.

Non-coding RNA Research, Journal Year: 2024, Volume and Issue: 10, P. 70 - 90

Published: Sept. 6, 2024

Language: Английский

Citations

3
Regulation of IFNβ expression: focusing on the role of its promoter and transcription regulators DOI Creative Commons

Jiqiang Fan,

Qiumei Li,

Jiadi Liang

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: June 15, 2023

IFNβ is a single-copy gene without an intron. Under normal circumstances, it shows low or no expression in cells. It upregulated only when the body needs stimulated. Stimuli bind to pattern recognition receptors (PRRs) and pass via various signaling pathways several basic transcriptional regulators, such as IRFs, NF-кB, AP-1. Subsequently, regulators enter nucleus regulatory elements of promoter. After modifications, position nucleosome altered complex assembled activate expression. However, regulation involves network. For study immunity diseases, important understand how transcription factors through specific forms, which cells are involved regulation, what occurs during assembly enhancers complexes, possible mechanisms after transcription. Thus, this review focuses on activation In addition, we discuss impact biology.

Language: Английский

Citations

4
Bridging autoimmunity and epigenetics: The influence of lncRNA MALAT1 DOI
Syam Mohan, Mohammed Ageeli Hakami, Hamad Ghaleb Dailah

et al.

Pathology - Research and Practice, Journal Year: 2023, Volume and Issue: 254, P. 155041 - 155041

Published: Dec. 20, 2023

Language: Английский

Citations

2
LÚPUS ERITEMATOSO SISTÊMICO: CRITÉRIOS DIAGNÓSTICOS E OPÇÕES TERAPÊUTICAS DOI

Diogo Uechi Fukuda Okoti,

Leylinda Fernanda de Sousa Leopoldino Dantas,

Larissa Linhares Santos

et al.

Revista Contemporânea, Journal Year: 2024, Volume and Issue: 4(6), P. e4884 - e4884

Published: June 27, 2024

O Lúpus Eritematoso Sistêmico (LES) é uma doença autoimune complexa que acomete sortidas partes do organismo. Há o desencadeamento de inflamação e dano tecidual. A prevalência eminente no sexo feminino, em afrodescendentes, hispânicos asiáticos. diagnóstico LES se baseia critérios clínicos, laboratoriais imunológicos, sendo fundamental para iniciar tratamento adequado prevenir complicações graves. multidisciplinar visa controle sintomático, a redução da proteção dos tecidos acometidos. Esta análise sistemática literatura averiguou pesquisas publicadas, nos últimos 5 anos, inerentes conduta LES. Os inclusão abrangeram estudos humanos, múltiplos idiomas, resultando na seleção 13 relevantes. Destacam-se: classificação, biomarcadores genéticos, terapia com células-tronco mesenquimais novas opções terapêuticas. Perspectivas positivas são avanços terapêuticos. Entretanto, revelou gaps compreensão patologia, desse modo, há necessidade futuros mais refinados elucidação adequada.

Citations

0
Unveiling Urinary Biomarkers, Crucial Genes, and Immune Infiltration in Lupus Nephritis Patients DOI Creative Commons

Huiqiong Zeng,

Zhongyu Xie, Haofei Hu

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 17, 2023

Abstract Background This study was conducted to investigate the expression patterns and biological roles of urinary long non-coding RNAs (lncRNAs) messenger (mRNAs) in individuals diagnosed with lupus nephritis (LN). Methods The cohort comprised six participants: three systemic erythematosus (SLE) involving LN, SLE without healthy controls (CON). Microarray technology employed analyze mRNAs lncRNAs, thereby exploring alterations overall RNA expression. Functional insights into dysregulated differentially expressed (DEMs) associated LN were derived through gene ontology (GO) enrichment analysis, Kyoto Encyclopedia Genes Genomes (KEGG) pathway set analysis (GSEA). Furthermore, construction a protein-protein interaction (PPI) network accomplished using Search Tool for Retrieval Interacting Genes/Proteins (STRING). identification immune-related cell types facilitated by Single-sample Gene Set Enrichment Analysis (ssGSEA). To predict potential drug candidates based on genes (DEGs), Connectivity Map (CMap) conducted. Results Within urine samples patients, total 247 602 lncRNAs exhibited differential relative control group. Among these, 83 down-regulated 141 up-regulated DEMs specifically discerned patients LN. GO highlighted processes such as transcriptional regulation, intrinsic apoptotic signaling pathways response DNA damage, regulation mitophagy. KEGG primarily revealed protein processing within endoplasmic reticulum, apoptosis, P53 pathway. Co-expression PPI suggested that nodes higher degrees connectivity concentrated related apoptosis autophagy. An assessment immune infiltration unveiled correlation between activated B cells CD56 dim natural killer (NK) pathogenesis. prediction drugs implicated inhibition mechanistic Aurora kinase A ( AURKA ) primary targeted intervention. molecular docking process confirmed robust binding activity hub genes' components. Conclusion has illuminated distinct profiles (LN) patients. These profiles, particularly context autophagy, involvement, provide valuable LN's underlying mechanisms. therapeutic targets, , offers promising directions future interventions management.

Language: Английский

Citations

0