Ethylene-mediated nitric oxide depletion pre-adapts plants to hypoxia stress

Nature Communications, Journal Year: 2019, Volume and Issue: 10(1)

Published: Sept. 5, 2019

Timely perception of adverse environmental changes is critical for survival. Dynamic in gases are important cues plants to sense perturbations, such as submergence. In Arabidopsis thaliana, oxygen and nitric oxide (NO) control the stability ERFVII transcription factors. proteolysis regulated by N-degron pathway mediates adaptation flooding-induced hypoxia. However, how detect transduce early submergence signals remains elusive. Here we show that can rapidly through passive ethylene entrapment use this signal pre-adapt impending Ethylene enhance prior hypoxia increasing NO-scavenger PHYTOGLOBIN1. This ethylene-mediated NO depletion consequent accumulation pre-adapts survive subsequent Our results reveal biological link between three gaseous regulation flooding survival identifies key regulatory targets stress could be pivotal developing flood-tolerant crops.

Language: Английский

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Vascular transcription factors guide plant epidermal responses to limiting phosphate conditions

Science, Journal Year: 2020, Volume and Issue: 370(6518)

Published: Sept. 17, 2020

Roots primed for better phosphate uptake Phosphate is a key resource plants, and remediating deficiency drives considerable fertilizer use. In low-phosphate conditions, roots make more root hairs, which makes them able to take up what little can be found. Wendrich et al. performed single-cell transcriptomics on the developing Arabidopsis queried resulting gene-expression atlas responses related vascular development. The authors found that signals regulating hair development began in inner vasculature of with transcription factors drove production hormone cytokinin. Response cascades identified through transcriptome database pointed genes epidermal cells regulate Science , this issue p. eaay4970

Language: Английский

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Identification of cell populations necessary for leaf-to-leaf electrical signaling in a wounded plant

Proceedings of the National Academy of Sciences, Journal Year: 2018, Volume and Issue: 115(40), P. 10178 - 10183

Published: Sept. 18, 2018

Significance Numerous modes of long-distance electrical signaling exist in nature. The best known these, axonal conduction, requires one primary cell population, i.e., neurons. In contrast, the types that mediate leaf-to-leaf wounded plants have not been defined rigorously. Using genetic approaches, we find two distinct populations cells vasculature matrix are needed to perform this function. Surprisingly, these do contact each other directly. As further plant wound response, found wound-induced membrane depolarizations preceded large intravasculature calcium fluxes. We reveal a two-cell-type mode leaves and discuss parallels differences outside kingdom.

Language: Английский

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CRISPR-TSKO: A Technique for Efficient Mutagenesis in Specific Cell Types, Tissues, or Organs in Arabidopsis

The Plant Cell, Journal Year: 2019, Volume and Issue: 31(12), P. 2868 - 2887

Published: Sept. 27, 2019

Detailed functional analyses of many fundamentally important plant genes via conventional loss-of-function approaches are impeded by the severe pleiotropic phenotypes resulting from these losses. In particular, mutations in that required for basic cellular functions and/or reproduction often interfere with generation homozygous mutant plants, precluding further studies. To overcome this limitation, we devised a clustered regularly interspaced short palindromic repeats (CRISPR)-based tissue-specific knockout system, CRISPR-TSKO, enabling somatic particular cell types, tissues, and organs. Arabidopsis (

Language: Английский

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Coordination between microbiota and root endodermis supports plant mineral nutrient homeostasis

Science, Journal Year: 2020, Volume and Issue: 371(6525)

Published: Nov. 20, 2020

Plant roots and animal guts have evolved specialized cell layers to control mineral nutrient homeostasis. These must tolerate the resident microbiota while keeping homeostatic integrity. Whether how root diffusion barriers in endodermis, which are critical for balance of plants, coordinate with is unknown. We demonstrate that genes controlling endodermal function model plant Arabidopsis thaliana contribute microbiome assembly. characterized a regulatory mechanism differentiation driven by profound effects on Furthermore, we this linked microbiota's capacity repress responses phytohormone abscisic acid root. Our findings establish endodermis as hub coordinating assembly mechanisms.

Language: Английский

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Genetic analysis of the Arabidopsis TIR1/AFB auxin receptors reveals both overlapping and specialized functions

eLife, Journal Year: 2020, Volume and Issue: 9

Published: Feb. 18, 2020

The TIR1/AFB auxin co-receptors mediate diverse responses to the plant hormone auxin. Arabidopsis genome encodes six proteins representing three of four clades that were established prior angiosperm radiation. To determine role these in development we performed an extensive genetic analysis involving generation and characterization all possible multiply-mutant lines. We find loss results early embryo defects eventually seed abortion, yet a single wild-type allele TIR1 or AFB2 is sufficient support growth throughout development. Our reveals functional overlap between even most distantly related genes except for AFB1. Surprisingly, AFB1 has specialized function rapid auxin-dependent inhibition root phase gravitropism. This activity may be difference subcellular localization compared other members family.

Language: Английский

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DNA methylation-free Arabidopsis reveals crucial roles of DNA methylation in regulating gene expression and development

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: March 14, 2022

Abstract A contribution of DNA methylation to defense against invading nucleic acids and maintenance genome integrity is uncontested; however, our understanding the extent involvement this epigenetic mark in genome-wide gene regulation plant developmental control incomplete. Here, we knock out all five known methyltransferases Arabidopsis , generating methylation-free plants. This quintuple mutant exhibits a suite defects, unequivocally demonstrating that essential for multiple aspects development. We show CG non-CG are required plethora biological processes, including pavement cell shape, endoreduplication, death, flowering, trichome morphology, vasculature meristem development, root fate determination. Moreover, find has strong dose-dependent effect on expression repression transposable elements. Taken together, results demonstrate dispensable survival but proper processes.

Language: Английский

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GDSL-domain proteins have key roles in suberin polymerization and degradation

Nature Plants, Journal Year: 2021, Volume and Issue: 7(3), P. 353 - 364

Published: March 8, 2021

Language: Английский

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Synthetic genetic circuits as a means of reprogramming plant roots

Science, Journal Year: 2022, Volume and Issue: 377(6607), P. 747 - 751

Published: Aug. 11, 2022

The shape of a plant's root system influences its ability to reach essential nutrients in the soil and acquire water during drought. Progress engineering plant roots optimize nutrient acquisition has been limited by our capacity design build genetic programs that alter growth predictable manner. We developed collection synthetic transcriptional regulators for plants can be compiled create circuits. These circuits control gene expression performing Boolean logic operations used predictably structure. This work demonstrates potential across tissues reprogram growth.

Language: Английский

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Burning questions for a warming and changing world: 15 unknowns in plant abiotic stress

The Plant Cell, Journal Year: 2022, Volume and Issue: 35(1), P. 67 - 108

Published: Aug. 26, 2022

We present unresolved questions in plant abiotic stress biology as posed by 15 research groups with expertise spanning eco-physiology to cell and molecular biology. Common themes of these include the need better understand how plants detect water availability, temperature, salinity, rising carbon dioxide (CO2) levels; environmental signals interface endogenous signaling development (e.g. circadian clock flowering time); this integrated controls downstream responses stomatal regulation, proline metabolism, growth versus defense balance). The plasma membrane comes up frequently a site key transport events mechanosensing lipid-derived signaling, aquaporins). Adaptation extremes CO2 affects hydraulic architecture transpiration, well root shoot morphology, ways not fully understood. Environmental adaptation involves tradeoffs that limit ecological distribution crop resilience face changing increasingly unpredictable environments. Exploration diversity within among species can help us know which represent fundamental limits ones be circumvented bringing new trait combinations together. Better defining what constitutes beneficial resistance different contexts making connections between genes phenotypes, laboratory field observations, are overarching challenges.

Language: Английский

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