Phosphate deficiency induced by infection promotes synthesis of anthracnose-resistant anthocyanin-3-O-galactoside phytoalexins in the Camellia sinensis plant DOI Creative Commons
Tongtong Li,

Shenrong Wang,

Dandan Shi

et al.

Horticulture Research, Journal Year: 2023, Volume and Issue: 10(12)

Published: Dec. 1, 2023

Abstract Tea (Camellia sinensis) is a well-known beverage crop rich in polyphenols with health benefits for humans. Understanding how tea participate plant resistance beneficial to breeding resistant varieties and uncovering the mechanisms. Here, we report that Colletotrichum infection-induced ‘pink ring’ symptom appeared outside lesion, which highly likely occur cultivars. By identifying morphological feature-specific metabolites pink ring their association disease resistance, analysis of between metabolite gene expression, study revealed accumulation anthocyanin-3-O-galactosides, red phytotoxin compounds anthracnose, plays pivotal role hypersensitive response surrounding infection sites plants. The results genetic manipulation showed expression CsF3Ha, CsANSa, CsUGT78A15, CsUGT75L43, CsMYB113, are involved anthocyanin biosynthesis, positively correlated anthracnose-resistance formation ring. Further phosphorus quantification fertilization experiments confirmed phosphate deficiency caused by anthracnose occurrence Genetic studies indicated altering levels Pi transporter proteins (CsPHT2-1, CsPHT4;4) deprivation transcription factors (CsWRKY75-1, CsWRKY75-2, CsMYB62-1) enhances This article provides first evidence anthocyanin-3-O-galactosides anthracnose-resistant phytoalexins among various plants, this presents an approach genes where transformation challenging.

Language: Английский

The complexities of proanthocyanidin biosynthesis and its regulation in plants DOI Creative Commons
Keji Yu,

Yushuang Song,

Jinxing Lin

et al.

Plant Communications, Journal Year: 2022, Volume and Issue: 4(2), P. 100498 - 100498

Published: Nov. 26, 2022

Proanthocyanidins (PAs) are natural flavan-3-ol polymers that contribute protection to plants under biotic and abiotic stress, benefits human health, bitterness astringency food products. They also potential targets for carbon sequestration climate mitigation. In recent years, from model species commercial crops, research has moved closer elucidating the flux control channeling, subunit biosynthesis polymerization, transport mechanisms, regulatory networks involved in plant PA metabolism. This review extends conventional understanding with findings provide new insights address lingering questions focus strategies manipulating traits plants.

Language: Английский

Citations

56
Single‐cell transcriptome atlas reveals developmental trajectories and a novel metabolic pathway of catechin esters in tea leaves DOI Creative Commons
Qiang Wang, Yi Wu,

Anqi Peng

et al.

Plant Biotechnology Journal, Journal Year: 2022, Volume and Issue: 20(11), P. 2089 - 2106

Published: July 10, 2022

The tea plant is an economically important woody beverage crop. unique taste of evoked by certain metabolites, especially catechin esters, whereas their precise formation mechanism in different cell types remains unclear. Here, a fast protoplast isolation method was established and the transcriptional profiles 16 977 single cells from 1st 3rd leaves were investigated. We first identified 79 marker genes based on six isolated tissues constructed transcriptome atlas, mapped developmental trajectories further delineated distribution during leaf differentiation associated with fate transformation. Interestingly, eight differently expressed found to co-exist at four branch points. Genes involved biosynthesis metabolites showed cell- development-specific characteristics. An unexpected ester glycosyltransferase characterized for time plants gene co-expression network mesophyll cells. Thus, single-cell landscape crop leave reported novel metabolism pathway esters discovered.

Language: Английский

Citations

54
Gene mining and genomics-assisted breeding empowered by the pangenome of tea plant Camellia sinensis DOI
Shuai Chen, Pengjie Wang, Weilong Kong

et al.

Nature Plants, Journal Year: 2023, Volume and Issue: 9(12), P. 1986 - 1999

Published: Nov. 27, 2023

Language: Английский

Citations

36
Special tea products featuring functional components: Health benefits and processing strategies DOI

Gaozhong Yang,

Qing Meng, Jiang Shi

et al.

Comprehensive Reviews in Food Science and Food Safety, Journal Year: 2023, Volume and Issue: 22(3), P. 1686 - 1721

Published: March 1, 2023

Abstract The functional components in tea confer various potential health benefits to humans. To date, several special products featuring (STPFCs) have been successfully developed, such as O ‐methylated catechin‐rich tea, γ‐aminobutyric acid‐rich low‐caffeine and selenium‐rich products. STPFCs some unique enhanced when compared with conventional products, which can meet the specific needs preferences of different groups huge market potential. processing strategies improve by regulating component content an active area research food science. fresh leaves varieties rich are used raw materials, technologies employed prepare STPFCs. Huge progress has achieved development these However, current status not yet systematically reviewed. Here, studies on comprehensively reviewed a focus their strategies. Additionally, other chemical be developed into teas application industry discussed. Finally, suggestions promises challenges for future study provided. This paper might shed light Future should screening varieties, identifying new components, evaluating health‐promoting effects, improving flavor quality, elucidating interactions between components.

Language: Английский

Citations

30
Quantitative fusion omics reveals that refrigeration drives methionine degradation through perturbing 5-methyltetrahydropteroyltriglutamate-homocysteine activity DOI Creative Commons
Wei Jia,

Xixuan Wu,

Ning Liu

et al.

Food Chemistry, Journal Year: 2022, Volume and Issue: 409, P. 135322 - 135322

Published: Dec. 24, 2022

Language: Английский

Citations

32
Anthocyanin metabolism and its differential regulation in purple tea (Camellia sinensis) DOI
Xiaoxiang Li, Ze-Yu Li,

Wan Zhu

et al.

Plant Physiology and Biochemistry, Journal Year: 2023, Volume and Issue: 201, P. 107875 - 107875

Published: July 9, 2023

Language: Английский

Citations

20
Characterization of thermostable carboxypeptidase from high-altitude hot spring metagenome DOI
Atif Khurshid Wani, Chirag Chopra, Mushtaq Ahmad Ansari

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 276, P. 133974 - 133974

Published: July 18, 2024

Language: Английский

Citations

7
Genomic variation of 363 diverse tea accessions unveils the genetic diversity, domestication, and structural variations associated with tea adaptation DOI
Wei Tong, Yanli Wang, Fangdong Li

et al.

Journal of Integrative Plant Biology, Journal Year: 2024, Volume and Issue: 66(10), P. 2175 - 2190

Published: July 11, 2024

ABSTRACT Domestication has shaped the population structure and agronomic traits of tea plants, yet complexity genetic variation that determines these remains unclear. We here investigated resequencing data 363 diverse accessions collected extensively from almost all distributions found plants was divided into eight subgroups, which were basically consistent with their geographical distributions. The diversity in China decreased southwest to east as latitude increased. Results also indicated Camellia sinensis var. assamica (CSA) illustrated divergent selection signatures (CSS). domesticated genes CSA mainly involved leaf development, flavonoid alkaloid biosynthesis, while CSS participated amino acid metabolism, aroma compounds cold stress. Comparative genomics further identified ~730 Mb novel sequences, generating 6,058 full‐length protein‐encoding genes, significantly expanding gene pool plants. discovered 217,376 large‐scale structural variations 56,583 presence absence (PAVs) across accessions, some associated quality stress resistance. Functional experiments demonstrated two PAV ( CSS0049975 CSS0006599 ) likely drive trait diversification tolerance between overall findings not only revealed domestication but underscored vital role plant traits.

Language: Английский

Citations

6
Tea Plantation Intercropping Legume Improves Soil Ecosystem Multifunctionality and Tea Quality by Regulating Rare Bacterial Taxa DOI Creative Commons
Ting Wang,

Yu Duan,

Xiaogang Lei

et al.

Agronomy, Journal Year: 2023, Volume and Issue: 13(4), P. 1110 - 1110

Published: April 13, 2023

Microorganisms play essential roles in soil-ecosystem multifunctionality. However, the contribution of their community assembly processes, composition, diversity, and keystone species to ecosystem multifunctionality is unclear, especially tea-plantation ecosystems. In order assess effects various intercropping patterns (tea-plant monoculture tea plants, respectively, intercropped with soybean, soybean—milk vetch, soybean—red clover, soybean—smooth vetch) on soil rare abundant taxa, a field experiment was carried out. We found that plantation legumes improved by altering environment, ultimately benefited nutrient absorption quality improvement leaves. Whether it bacteria or fungi, taxa had higher proportion deterministic processes than taxa. Additionally, practices changed bacterial were assembled shifted from variable selection homogeneous dispersal. Intercropping significantly fungal communities’ α-diversity Increasing legume practice enhanced dissimilarity affecting pH, ammonium nitrogen, nitrate nitrogen. Rare β-diversity exhibited stronger positive relationships (both average multi-threshold approaches) compared corresponding Furthermore, under different closely related operational taxonomic units, Chloroflexi. Our results emphasize disparate feedbacks diverse practices, as well important connection between

Language: Английский

Citations

14
A flavonoid metabolon: cytochrome b5 enhances B‐ring trihydroxylated flavan‐3‐ols synthesis in tea plants DOI

Haixiang Ruan,

Liping Gao, Fang Zhou

et al.

The Plant Journal, Journal Year: 2024, Volume and Issue: 118(6), P. 1793 - 1814

Published: March 10, 2024

Flavan-3-ols are prominent phenolic compounds found abundantly in the young leaves of tea plants. The enzymes involved flavan-3-ol biosynthesis plants have been extensively investigated. However, localization and associations these numerous functional within cells largely neglected. In this study, we aimed to investigate synthesis flavan-3-ols plants, particularly focusing on epigallocatechin gallate. Our analysis involving DESI-MSI method reveal a distinct distribution pattern B-ring trihydroxylated flavonoids, primarily concentrated outer layer buds. Subcellular showed that CsC4H, CsF3'H, CsF3'5'H localizes endoplasmic reticulum. Protein-protein interaction studies demonstrated direct between cytoplasmic (CHS, CHI, F3H, DFR, FLS, ANR), highlighting their interactions biosynthetic pathway. Notably, CsF3'5'H, enzyme for trihydroxylation, did not directly interact with other enzymes. We identified cytochrome b

Language: Английский

Citations

5