Genome-wide identification and analysis of the NF-Y transcription factor family reveal its potential roles in tobacco ( Nicotiana tabacum L.)

Plant Signaling & Behavior, Journal Year: 2025, Volume and Issue: 20(1)

Published: Jan. 16, 2025

Nuclear Factor Y (NF-Y) represents a group of transcription factors commonly present in higher eukaryotes, typically consisting three subunits: NF-YA, NF-YB, and NF-YC. They play crucial roles the embryonic development, photosynthesis, flowering, abiotic stress responses, other essential processes plants. To better understand genome-wide NF-Y domain-containing proteins, protein physicochemical properties, chromosomal localization, synteny, phylogenetic relationships, genomic structure, promoter cis-elements, interaction network NtNF-Ys tobacco (Nicotiana tabacum L.) were systematically analyzed. In this study, we identified 58 tobacco, respectively, divided into subfamilies corresponding to their relationships. Their tissue specificity expression pattern analyses for leaf drought saline-alkali stress, ABA response carried out using RNA-seq or qRT-PCR. These findings illuminate role regulating plant tolerance, response. This study offers new insights enhance our understanding identify potential genes involved as well tolerance

Language: Английский

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Systematic Analysis of Stay-Green Genes in Six Ipomoea Species Reveals the Evolutionary Dynamics, Carotenoid and Anthocyanin Accumulation, and Stress Responses of Sweet Potato

Genes, Journal Year: 2025, Volume and Issue: 16(3), P. 266 - 266

Published: Feb. 24, 2025

Background/Objectives: Stay-green proteins (SGRs) play a vital role in regulating plant chlorophyll degradation and senescence. However, this gene family has not been explored Ipomoea species sweet potato. Methods: A total of 19 SGR genes were identified using Basic Local Alignment Search Tool (BLAST) methods. The proteins’ physiological properties, evolutionary phylogenetic relationships, conserved domain motifs, structures, collinearity, promoter cis-elements systematically analyzed. Moreover, expression patterns protein interaction network analyses performed for Results: In study, we SGRs six species. These divided into four subgroups according to their relationships. Domian analysis revealed that had the “stay-green” domain. Gene structure showed similar structures. collinearity originated from two genes, with one undergoing duplication during evolution history; moreover, experienced rearrangement throughout process Cis-elements related pigment biosynthesis hormone stress responses found. addition, pattern IbSGRs, especially IbSGR1, IbSGR2, IbSGR3, might an important accumulation. could also respond (i.e., cold, drought, salt) take part crosstalk abscisic acid (ABA), methyl jasmonate (MeJA), salicylic (SA)). Conclusions: Taken together, findings study provide new insights further understanding functions candidate accumulation tolerance potatoes.

Language: Английский

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Integrated Analysis of Hormone Metabolomics and Transcriptomics Reveals the Differential Mechanism of Ginger (Zingiber officinale Roscoe) Rhizome Bulking

Agronomy, Journal Year: 2025, Volume and Issue: 15(4), P. 779 - 779

Published: March 22, 2025

As a product and reproductive organ of ginger (Zingiber officinale Roscoe), the degree rhizome bulking is key factor in determining yield economic value ginger. There are few studies on regulatory mechanism This study aims to identify hormone that regulates screen for critical hormone-associated genes. research subjects, two accessions—large (L) with thickened small (S) slender rhizome—were derived from same parent plant. The ploidy differences between determine variations gene dosage as well differential expression patterns. levels eight hormones L S during different growth stages were analyzed. Differentially expressed genes (DEGs) identified by combining third-generation transcriptome sequencing technology (PacBio SMART) quantitative real time PCR (qRT-PCR). Through screening methods such Orthogonal Partial Least Squares Discriminant Analysis (OPLS-DA), auxin, cytokinin, salicylic acid across various periods. Among these, changes IAA level showed positive correlation bulking. them, change was positively correlated Transcriptome analysis combined qRT-PCR revealed auxin response ZoARF7 ZoARF23 likely act regulators provides theoretical foundation elucidating molecular mechanisms underlying hormone-mediated

Language: Английский

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The Ibmyb52/Ibarf11l-Ibdrm1 Module Negatively Regulates the Root Development of Sweetpotato

Published: Jan. 1, 2025

Language: Английский

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The Ibmyb52/Ibarf11l-Ibdrm1 Module Negatively Regulates the Root Development of Sweetpotato

Published: Jan. 1, 2025

Language: Английский

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The IbDof2.1–IbABF2 module regulates abscisic acid responses and proline biosynthesis to enhance drought tolerance in sweetpotato

The Plant Journal, Journal Year: 2025, Volume and Issue: 122(3)

Published: May 1, 2025

SUMMARY Drought is a major abiotic stress that impairs plant growth and development. Developing drought‐tolerant crop varieties an important goal of breeders. Transcription factors belonging to the DNA‐binding with one zinc finger (Dof) family regulate responses However, roles regulatory mechanisms Dof2.1 members in tolerance are still unclear. Here, we cloned IbDof2.1 gene from sweetpotato found its overexpression significantly enhanced drought sweetpotato, whereas ‐RNA interference (RNAi) plants displayed opposite phenotype. The ‐overexpression showed increased abscisic acid (ABA) proline contents stomatal sensitivity ABA decreased H 2 O accumulation. Furthermore, interacted ABA‐binding factor (IbABF2) promoted expression biosynthesis IbP5CS1 increase content, further activating reactive oxygen species (ROS) scavenging system. These results suggest IbDof2.1–IbABF2 module induces closure activates ROS system by regulating enhance sweetpotato. Our findings provide novel insights into plants.

Language: Английский

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A Small Auxin-Up RNA Gene, IbSAUR36, Regulates Adventitious Root Development in Transgenic Sweet Potato

Genes, Journal Year: 2024, Volume and Issue: 15(6), P. 760 - 760

Published: June 10, 2024

Small auxin-upregulated RNAs (

Language: Английский

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Genome and Transcriptome Analysis of NF-Y Transcription Factors in Sweet Potato under Salt Stress

Horticulturae, Journal Year: 2024, Volume and Issue: 10(8), P. 798 - 798

Published: July 28, 2024

Nuclear factor Y (NF-Y) is a heterotrimeric complex composed of three unique subunits: NF-YA, NF-YB, and NF-YC. This transcription binds to the CCAAT box eukaryotic promoters, playing crucial role in various biological processes plants. Despite its importance, NF-Y gene family has not been reported sweet potato (Ipomoea batatas) genome, an important food energy crop. Understanding function potatoes could provide valuable insights for genetic improvement yield enhancement. To address this gap, our research aimed comprehensively catalog characterize genes potatoes, which we refer as ‘IbNF-Y’, where ‘Ib’ denotes Ipomoea batatas. A total 37 NF-Ys were identified, including 11 21 5 NF-YC members, their phylogeny, structure, chromosomal distribution, conserved motifs analyzed. Additionally, assessed expression patterns under salt stress both light dark conditions using transcriptome sequencing. Notably, discovered that certain IbNF-Y showed significant changes stress, suggesting potential roles potato’s adaptation saline environments. Furthermore, work enriches genomics on contributes knowledge broader scientific community Convolvulaceae family.

Language: Английский

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The molecular coordination of tuberization: Current status and future directions

Current Opinion in Plant Biology, Journal Year: 2024, Volume and Issue: 82, P. 102655 - 102655

Published: Nov. 8, 2024

Language: Английский

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