Treadmill training does not enhance skeletal muscle recovery following disuse atrophy in older male mice

Frontiers in Physiology, Journal Year: 2023, Volume and Issue: 14

Published: Oct. 24, 2023

Introduction: A hallmark of aging is poor muscle recovery following disuse atrophy. Efficacious strategies to enhance atrophy in are non-existent. Prior exercise training could result favorable morphological and cellular adaptations that may promote aging. Here, we characterized the impact on skeletal inflammatory metabolic profiles remodeling function, together with femoral artery reactivity prior from aged male mice. We hypothesized 12 weeks treadmill mice would improve at baseline during atrophy, resulting improved regrowth. Methods: Physical performance, ex vivo vascular tissue organ mass, hindlimb (macrophage, satellite cell, capillary, myofiber size, fibrosis), proteolytic, inflammatory, transcripts were evaluated exercise-trained sedentary Results: found (vs. mice), capacity physical function increased, fat mass decreased, endothelial improved. However, did not alter tibialis anterior or gastrocnemius transcriptional profile, macrophage, capillarity collagen content, size only tended increase Conclusion: While old whole-body composition as anticipated, had limited response

Language: Английский

---

Muscle fiber Myc is dispensable for muscle growth and forced expression severely perturbs homeostasis

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: March 14, 2024

ABSTRACT The oncogenic transcription factor Myc stimulates many growth processes including cell cycle progression and ribosome biogenesis. expression is low in adult skeletal muscle, but upregulated upon stimuli. Furthermore, muscle fiber overexpression recapitulates aspects of growth-related gene expression, suggesting may mediate pro-growth responses to anabolic stimuli, such as exercise. Here, we tested this hypothesis by examining mouse models which was specifically eliminated or overexpressed fibers stem cells (MuSC). While increased during MuSCs required for successful regeneration, dispensable post-natal, mechanical overload PKB/Akt-induced mice. Similarly, constitutive did not promote hypertrophy, instead impaired structure function within days. These data question the role growth.

Language: Английский

---

Investigating age-related differences in muscles of Kazakh horse through transcriptome analysis

Gene, Journal Year: 2024, Volume and Issue: 919, P. 148483 - 148483

Published: April 24, 2024

This study conducted transcriptome sequencing on the skeletal muscles of three different anatomical locations across various growth stages to investigate impact ages crucial candidate genes and molecular mechanisms associated with muscle development in Kazakh horses. Sixteen horses were selected, they divided into four age groups, each biological replicates. Tissue samples from longest dorsal muscle, abdominal diaphragm collected for analysis. The results revealed differential mRNA expression between eight-month group (Group O) 10-year F), 434 up-regulated 322 down-regulated genes. In there 125 127 genes, while 73 70 this study, GO enrichment analysis focused processes. KEGG pathway highlighted Oxidative Phosphorylation annotating 37 differentially expressed (DEGs), including ATP5PF, NDUFB8, ATP5MG, all which down-regulated. For ECM-receptor interaction was enriched, 7 DEGs such as COL4A2, COL4A1, ITGA5. Hippo signaling 6 DEGs, SERPINE1, RASSF1, FZD10. provides robust data support a theoretical foundation comprehensive understanding influence

Language: Английский

---

Organ specificity and commonality of epigenetic aging in low- and high-running capacity rats

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: April 26, 2024

Abstract Background Epigenetic drift, which are gradual age-related changes in DNA methylation patterns, plays a significant role aging and diseases. However, the relationship between exercise, epigenetics, molecular mechanisms underlying their interactions poorly understood. The aim of this study was to investigate cardiorespiratory fitness (CRF), epigenetic aging, promoter individual genes across multiple organs selectively bred low- high-capacity runner (LCR HCR) aged rats. Methods In study, we performed reduced representation bisulfite sequencing (RRBS) on LCR HCR rats, focusing hippocampus, heart, soleus muscle, large intestine samples. We calculated various indicators, including rat clocks, global mean (GMM), entropy (MME), gene-specific methylation. Results trained available blood-derived RRBS data did not reflect differences CRF rats all four organs. observed organ specific GMM MME direction these opposite compared blood suggesting that high may mitigate an organ-specific manner. Notably exhibited most pronounced due CRF. also identified seven whose consistently influenced by Moreover, found age acceleration muscle significantly higher heart lower intestine. Finally, consistent Conclusions Our suggest associates with manner regulates organ-common findings provide important insights into biology emphasize need validate rejuvenation strategies context nature aging.

Language: Английский

---

Bioinformatics Analysis Identifies Key Genes in the Effect of Resistance Training on Female Skeletal Muscle Aging

Journal of Aging and Physical Activity, Journal Year: 2024, Volume and Issue: 32(4), P. 531 - 540

Published: April 29, 2024

Resistance training is used to combat skeletal muscle function decline in older adults. Few studies have been designed specific for females, resulting very limited treatment options atrophy aging women. Here, we analyzed the gene expression profiles of samples from sedentary young women, and resistance-trained using microarray data public database. A total 45 genes that were differentially expressed during female reversed by resistance identified. Functional pathway enrichment analysis, protein-protein interaction network receiver operating characteristic analysis performed reveal key pathways involved effects on aging. The collagen COL1A1, COL3A1, COL4A1 identified important regulators training, modulating multiple signaling pathways, such as PI3 kinase-Akt signaling, focal adhesions, extracellular matrix-receptor interactions, relaxin signaling. Interestingly, CDKN1A TP63 increased aging, further upregulated suggesting they may negatively affect outcomes. Our findings provide novel insights into molecular mechanisms identify potential biomarkers targets clinical intervention.

Language: Английский

---