Cited by 稀疏结构光照明三维层析显微技术

Computational optical phase imaging: From digital holographic interferometry to intensity diffraction tomography DOI

Chao Zuo,

Qian Chen

Chinese Science Bulletin (Chinese Version), Journal Year: 2023, Volume and Issue: 68(25), P. 3240 - 3243

Published: Aug. 17, 2023

Language: Английский

Citations

基于三角级数拟合的间接飞行时间相机谐波误差校正方法 DOI

姜兆祥 Jiang Zhaoxiang,

宋展 Song Zhan,

肖振中 Xiao Zhenzhong

et al.

Laser & Optoelectronics Progress, Journal Year: 2023, Volume and Issue: 60(8), P. 0811028 - 0811028

Published: Jan. 1, 2023

Citations

Quantitative real-time phase microscopy for extended depth-of-field imaging based on the 3D single-shot differential phase contrast (ssDPC) imaging method DOI

Jingfan Wang, Xing Zhao, Yan Wang

et al.

Optics Express, Journal Year: 2023, Volume and Issue: 32(2), P. 2081 - 2081

Published: Dec. 21, 2023

Optical diffraction tomography (ODT) is a promising label-free imaging method capable of quantitatively measuring the three-dimensional (3D) refractive index distribution transparent samples. In recent years, partially coherent ODT (PC-ODT) has attracted increasing attention due to its system simplicity and absence laser speckle noise. Quantitative phase (QPI) technologies represented by Fourier ptychographic microscopy (FPM), differential contrast (DPC) intensity (IDT) need collect several or hundreds images, which usually introduce motion artifacts when shooting fast-moving targets, leading decrease in image quality. Hence, quantitative real-time (qRPM) for extended depth field (DOF) based on 3D single-shot (ssDPC) proposed this research study. qRPM incorporates microlens array (MLA) simultaneously spatial information angular information. subsequent optical processing, deconvolution used obtain stacks under different illumination angles raw light image. Importing obtained stack into DPC model able finally distribution. The captured four-dimensional enables reconstruction single snapshot extending DOF qRPM. capability experimental verified samples, achieve single-exposure with an 160 µm nearly 30 times higher than traditional microscope system.

Language: Английский

Citations

Live-cell analysis framework for quantitative phase imaging with slightly off-axis digital holographic microscopy DOI

Qian Shen,

Zhuoshi Li, Jiasong Sun

et al.

Frontiers in Photonics, Journal Year: 2022, Volume and Issue: 3

Published: Dec. 22, 2022

Label-free quantitative phase imaging is an essential tool for studying in vitro living cells various research fields of life sciences. Digital holographic microscopy (DHM) a non-destructive full-field technique that provides images by directly measuring the optical path differences, which facilitates cell segmentation and allows determination several important cellular physical features, such as dry mass. In this work, we present systematic analysis framework live-cell morphological characterization, terms LAF (live-cell framework). All image processing algorithms involved are implemented on high-resolution artifact-free obtained our previously proposed slightly off-axis system (FPDH) associated reconstruction methods. A highly robust automated method applied to extract valid region, followed determine properties, including area, perimeter, irregularity, volume mass, each individual cell. Experiments live HeLa demonstrate validity effectiveness presented framework, revealing its potential diverse biomedical applications.

Language: Английский

Citations

稀疏结构光照明三维层析显微技术 DOI

雷云泽 Lei Yunze,

郜鹏 Gao Peng,

刘星 Liu Xing

et al.

Laser & Optoelectronics Progress, Journal Year: 2023, Volume and Issue: 60(8), P. 0811016 - 0811016

Published: Jan. 1, 2023

Citations