The coral microbiome in sickness, in health and in a changing world

Nature Reviews Microbiology, Journal Year: 2024, Volume and Issue: 22(8), P. 460 - 475

Published: March 4, 2024

Language: Английский

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Coupled carbon and nitrogen cycling regulates the cnidarian–algal symbiosis

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 1, 2023

Efficient nutrient recycling underpins the ecological success of cnidarian-algal symbioses in oligotrophic waters. In these symbioses, nitrogen limitation restricts growth algal endosymbionts hospite and stimulates their release photosynthates to cnidarian host. However, mechanisms controlling availability role symbiosis regulation remain poorly understood. Here, we studied metabolic symbiotic cycling sea anemone Aiptasia by experimentally altering labile carbon a series experiments. Combining

Language: Английский

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Destabilization of mutualistic interactions shapes the early heat stress response of the coral holobiont

Microbiome, Journal Year: 2025, Volume and Issue: 13(1)

Published: Jan. 31, 2025

The stability of the symbiotic relationship between coral and their dinoflagellate algae (Symbiodiniaceae) is disrupted by ocean warming. Although thermal response depends on complex interactions host, Symbiodiniaceae prokaryotes, mechanisms underlying initial destabilization these symbioses are poorly understood. In a 2-month manipulative experiment, we exposed Porites lutea to gradually increasing temperatures corresponding 0-8 degree heating weeks (DHW) assessed holobiont using transcriptomics, microbial 16S rRNA gene sequencing physiological measurements. From early stages heat stress (< 1 DHW), increase in metabolic turnover shifted net heterotrophic state which algal-derived nutrients were insufficient meet host energy demands, resulting reduced performance at DHW. We postulate altered nutrient cycling also affected coral-associated community, with relative abundance Endozoicomonas bacteria declining under stress. Integration responses correlated this decline an expression ADP-ribosylation factor, suggesting that may underlie similar endosymbiotic regulatory processes. thermotolerance holobionts therefore influenced nutritional status its members interactions, identified interdependency highlights importance applying integrative approach guide reef conservation efforts. Video Abstract.

Language: Английский

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A carbon-nitrogen negative feedback loop underlies the repeated evolution of cnidarian–Symbiodiniaceae symbioses

Nature Communications, Journal Year: 2023, Volume and Issue: 14(1)

Published: Nov. 1, 2023

Symbiotic associations with Symbiodiniaceae have evolved independently across a diverse range of cnidarian taxa including reef-building corals, sea anemones, and jellyfish, yet the molecular mechanisms underlying their regulation repeated evolution are still elusive. Here, we show that despite independent evolution, hosts use same carbon-nitrogen negative feedback loop to control symbiont proliferation. Symbiont-derived photosynthates used assimilate nitrogenous waste via glutamine synthetase-glutamate synthase-mediated amino acid biosynthesis in carbon-dependent manner, which regulates availability nitrogen symbionts. Using nutrient supplementation experiments, provision additional carbohydrates significantly reduces density while ammonium promotes High-resolution metabolic analysis confirmed all co-incorporated glucose-derived 13C ammonium-derived 15N biosynthesis. Our results reveal general these symbioses provide parsimonious explanation for evolution.

Language: Английский

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Microbial Processes and Nutrient Uptake in the Coral Holobiont and Reef Ecosystems

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 113 - 130

Published: Jan. 1, 2025

Language: Английский

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Insights into the molecular mechanisms underlying the different heat tolerance of the scleractinian coral Pavona decussata

Coral Reefs, Journal Year: 2024, Volume and Issue: 43(2), P. 429 - 442

Published: March 4, 2024

Language: Английский

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Photosynthesis and other factors affecting the establishment and maintenance of cnidarian–dinoflagellate symbiosis

Philosophical Transactions of the Royal Society B Biological Sciences, Journal Year: 2024, Volume and Issue: 379(1901)

Published: March 18, 2024

Coral growth depends on the partnership between animal hosts and their intracellular, photosynthetic dinoflagellate symbionts. In this study, we used sea anemone Aiptasia , a laboratory model for coral biology, to investigate poorly understood mechanisms that mediate symbiosis establishment maintenance. We found initial colonization of both adult polyps larvae by compatible algal strain was more effective when algae were able photosynthesize long-term maintenance also depended photosynthesis. dark, cells taken up into host gastrodermal not rapidly expelled, but they seemed unable reproduce thus gradually lost. When confocal microscopy examine interaction with two strains cannot establish stable symbioses it appeared pre- post-phagocytosis involved. With one strain, entered gastric cavity be completely excluded from cells. other small numbers proliferate there slowly lost upon further incubation. asked if exclusion either incompatible could result simply cells' being too large accommodate. However, size distributions overlapped extensively. Moreover, examination macerates confirmed earlier reports individual expand accommodate multiple This article is part theme issue ‘Sculpting microbiome: how factors determine respond microbial colonization’.

Language: Английский

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Sea Anemone Membrane Attack Complex/Perforin Superfamily Demonstrates an Evolutionary Transitional State between Venomous and Developmental Functions

Molecular Biology and Evolution, Journal Year: 2024, Volume and Issue: 41(5)

Published: April 26, 2024

Abstract Gene duplication is a major force driving evolutionary innovation. A classic example generating new animal toxins via of physiological protein-encoding genes and recruitment into venom. While this process drives the innovation many venoms, reverse nonvenomous cells remains unresolved. Using comparative genomics, we find members Membrane Attack Complex Perforin Family (MAC) have been recruited venom-injecting (cnidocytes), in soft stony corals sea anemones, suggesting that ancestral MAC was cnidocyte expressed toxin. Further investigation model anemone Nematostella vectensis reveals three undergone Nematostella-specific duplications leading to their endomesodermal cells. Furthermore, simultaneous knockdown all endomesodermally MACs leads mis-development, supporting these paralogs function. By resolving history function Nematostella, provide first proof for from venom organismal development.

Language: Английский

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Ecosystem Microbiology of Coral Reefs

Coral reefs of the world, Journal Year: 2025, Volume and Issue: unknown, P. 131 - 144

Published: Jan. 1, 2025

Language: Английский

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Metabolic Shifts and Muscle Remodeling as Pro-Survival and Energy Compensation Strategies in Photosymbiotic Giant Clams after Bleaching

Environmental Science & Technology, Journal Year: 2025, Volume and Issue: unknown

Published: April 28, 2025

Tropical photosymbiotic giant clams are increasingly threatened by climate change, leading to widespread bleaching. Decline in density of symbionts caused mortality events symbiotic organisms; however, appear exhibit prior survival capacity against these detrimental effects. It remains unclear whether can mitigate the adverse impacts Herein, we found that bleaching after chronic heat stress induce remarkable changes and remodeling tissue clam Tridacna crocea. The structure unique muscle fibers with high collagen content, observed siphonal mantle animals, were negatively altered stress. These associated a metabolic shift from carbohydrates fatty acids amino as breakdown collagen-rich partially compensate for energy loss during Such was proposed be regulated AMP-activated protein kinase (AMPK) signaling FoxO-atrogin pathways. Overall, our study highlights pro-survival mechanism through plastic regulation, which likely contributes their relatively environmental resilience

Language: Английский

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