Cells Prioritize the Regulation of Cell Mass Density DOI Open Access

Jinyu Fu,

Na Qin, Yufei Wu

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

A cell's global physical state is characterized by its volume and dry mass. The ratio of cell mass to the density (CMD), which also a measure macromolecular crowding concentrations all proteins. Using Fluorescence eXclusion method (FXm) Quantitative Phase Microscopy (QPM), we investigate CMD dynamics after exposure sudden media osmolarity change. We find that while exhibit complex behavior osmotic shock, follows straightforward monotonic recovery in 48 hours. cell-cycle independent relies on coordinated adjustment protein synthesis growth rates. Surprisingly, rate decreases when increases. This result explained CMD-dependent nucleoplasm-cytoplasm transport, serves as negative regulatory feedback CMD. Na+/H+ exchanger NHE plays role regulating affecting both Taken together, reveal cells possess robust control system actively regulates during environmental

Language: Английский

Perspective on quantitative phase imaging to improve precision cancer medicine DOI Creative Commons
Yang Liu, Shikhar Uttam

Journal of Biomedical Optics, Journal Year: 2024, Volume and Issue: 29(S2)

Published: March 26, 2024

SignificanceQuantitative phase imaging (QPI) offers a label-free approach to non-invasively characterize cellular processes by exploiting their refractive index based intrinsic contrast. QPI captures this contrast translating associated shifts into intensity-based quantifiable data with nanoscale sensitivity. It holds significant potential for advancing precision cancer medicine providing quantitative characterization of the biophysical properties cells and tissue in natural states.AimThis perspective aims discuss increase our understanding development its response therapeutics. also explores new developments methods towards personalized therapy early detection.ApproachWe begin detailing technical advancements QPI, examining implementations across transmission reflection geometries retrieval methods, both interferometric non-interferometric. The focus then QPI's applications research, including dynamic cell mass drug assessment, risk stratification, in-vivo imaging.ResultsQPI has emerged as crucial tool medicine, offering insights tumor biology treatment efficacy. Its sensitivity detecting changes promise enhancing diagnostics, prognostication. future is envisioned integration artificial intelligence, morpho-dynamics, spatial biology, broadening impact research.ConclusionsQPI presents redefining diagnosis, monitoring, treatment. Future directions include harnessing high-throughput imaging, 3D realistic models, combining intelligence multi-omics extend capabilities. As result, stands at forefront research clinical application care.

Language: Английский

Citations

7
Nanoplastics Penetrate Human Bronchial Smooth Muscle and Small Airway Epithelial Cells and Affect Mitochondrial Metabolism DOI Open Access
Ewa Winiarska, Monika Chaszczewska-Markowska, Daniel Ghete

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(9), P. 4724 - 4724

Published: April 26, 2024

Micro- and nanoplastic particles, including common forms like polyethylene polystyrene, have been identified as relevant pollutants, potentially causing health problems in living organisms. The mechanisms at the cellular level largely remain to be elucidated. This study aims visualize nanoplastics bronchial smooth muscle (BSMC) small airway epithelial cells (SAEC), assess impact on mitochondrial metabolism. Healthy asthmatic human BSMC SAEC vitro cultures were stimulated with polystyrene (PS-NPs) of 25 or 50 nm size, for 1 24 h. Live cell, label-free imaging by holotomography microscopy respiration glycolysis assessment performed. Furthermore, NPs shown penetrate SAEC, along healthy diseased BSMC, they impaired bioenergetics induce dysfunction compared not treated NPs, changes oxygen consumption rate extracellular acidification rate. pose a serious threat penetrating tissues cells, affecting both oxidative glycolytic

Language: Английский

Citations

7
Raman Spectroscopy in Cellular and Tissue Aging Research DOI Creative Commons
Jeong Hee Kim, Daejong Yang, Seungman Park

et al.

Aging Cell, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 28, 2025

ABSTRACT The establishment of various molecular, physiological, and genetic markers for cellular senescence aging‐associated conditions has progressed the aging study. To identify such markers, a combination optical, proteomic‐, sequencing‐based tools is primarily used, often accompanying extrinsic labels. Yet, clinical detection at cellular, tissue levels are still lacking which profoundly hinders advancements in specific timely prevention aging‐related diseases pathologies. Raman spectroscopy, with its capability rapid, label‐free, non‐invasive analysis molecular compositions alterations cells tissues, holds considerable promise vivo applications. In this review, we present recent application spectroscopy to study tissues. We explore use related techniques detecting senescence, focusing on that accompany these processes. Subsequently, provide review identifying changes molecules within tissues organs.

Language: Английский

Citations

0
Innovative Approaches for Drug Discovery: Quantifying Drug Distribution and Response with Raman Imaging DOI
Erin L. Dunnington, Brian Wong, Dan Fu

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(20), P. 7926 - 7944

Published: April 16, 2024

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTInnovative Approaches for Drug Discovery: Quantifying Distribution and Response with Raman ImagingErin L. DunningtonErin DunningtonDepartment of Chemistry, University Washington, Seattle, Washington 98195, United StatesMore by Erin DunningtonView Biographyhttps://orcid.org/0009-0005-7063-8346, Brian S. WongBrian WongDepartment WongView Biographyhttps://orcid.org/0000-0001-6405-468X, Dan Fu*Dan FuDepartment States*[email protected]More FuView Biographyhttps://orcid.org/0000-0001-9243-8306Cite this: Anal. Chem. 2024, 96, 20, 7926–7944Publication Date (Web):April 16, 2024Publication History Received16 March 2024Accepted8 April 2024Revised8 2024Published online16 inissue 21 May 2024https://pubs.acs.org/doi/10.1021/acs.analchem.4c01413https://doi.org/10.1021/acs.analchem.4c01413review-articleACS PublicationsCopyright © 2024 American Chemical SocietyRequest reuse permissionsArticle Views732Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Anatomy,Biological imaging,Imaging,Lipids,Pharmaceuticals Get e-Alerts

Language: Английский

Citations

3
Optical Diffraction Tomography and Raman Confocal Microscopy for the Investigation of Vacuoles Associated with Cancer Senescent Engulfing Cells DOI Creative Commons
Silvia Ghislanzoni, Jeon Woong Kang, Arianna Bresci

et al.

Biosensors, Journal Year: 2023, Volume and Issue: 13(11), P. 973 - 973

Published: Nov. 7, 2023

Wild-type p53 cancer therapy-induced senescent cells frequently engulf and degrade neighboring ones inside a massive vacuole in their cytoplasm. After clearance of the internalized cell, persists, seemingly empty, for several hours. Despite large vacuoles being associated with cell death, this process is known to confer survival advantage engulfing cells, leading therapy resistance tumor relapse. Previous attempts resolve vacuolar structure visualize content using dyes were unsatisfying lack targets ineffective dye penetration and/or retention. Here, we overcame problem by applying optical diffraction tomography Raman spectroscopy MCF7 doxorubicin-induced cells. We demonstrated real ability phenotype complex microstructures, such as cell-in-cells vacuoles, detect chemical species extremely low concentrations within live completely label-free fashion. show that had density indistinguishable medium, but not instead contained diluted cell-derived macromolecules, could discern from medium fingerprint. Our approach useful noninvasive investigation (and other peculiar) unperturbed conditions, crucial better understanding biological processes.

Language: Английский

Citations

7
Pinpointing Fat Molecules: Advances in Coherent Raman Scattering Microscopy for Lipid Metabolism DOI

Xiangjie Huang,

Zexin Xue,

Delong Zhang

et al.

Analytical Chemistry, Journal Year: 2024, Volume and Issue: 96(20), P. 7945 - 7958

Published: May 3, 2024

ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTPinpointing Fat Molecules: Advances in Coherent Raman Scattering Microscopy for Lipid MetabolismXiangjie HuangXiangjie HuangCollege of Biomedical Engineering & Instrument Science, and Key Laboratory Ministry Education, Zhejiang University, Hangzhou 310027, ChinaMore by Xiangjie Huang, Zexin XueZexin XueCollege Xue, Delong ZhangDelong ZhangMOE Frontier Science Center Brain Brain-Machine Integration, ChinaZhejiang Micro-nano Quantum Chips Control, School Physics, Zhanghttps://orcid.org/0000-0002-9734-3573, Hyeon Jeong Lee*Hyeon LeeCollege ChinaMOE China*Email: [email protected]More Leehttps://orcid.org/0000-0002-2417-9338Cite this: Anal. Chem. 2024, 96, 20, 7945–7958Publication Date (Web):May 3, 2024Publication History Received15 March 2024Accepted24 April 2024Revised17 2024Published online3 May inissue 21 2024https://pubs.acs.org/doi/10.1021/acs.analchem.4c01398https://doi.org/10.1021/acs.analchem.4c01398review-articleACS PublicationsCopyright © 2024 American Chemical SocietyRequest reuse permissionsArticle Views356Altmetric-Citations-LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008 (both PDF HTML) across all institutions individuals. These metrics regularly updated to reflect usage leading up last few days.Citations number other articles citing this article, calculated Crossref daily. Find more information about citation counts.The Altmetric Attention Score is a quantitative measure attention that research has received online. Clicking on donut icon will load page at altmetric.com with additional details score social media presence given article. how calculated. Share Add toView InAdd Full Text ReferenceAdd Description ExportRISCitationCitation abstractCitation referencesMore Options onFacebookTwitterWechatLinked InRedditEmail Other access optionsGet e-Alertsclose SUBJECTS:Biological imaging,Cancer,Imaging,Lipids,Metabolism Get e-Alerts

Language: Английский

Citations

2
Label-free morpho-molecular phenotyping of living cancer cells by combined Raman spectroscopy and phase tomography DOI Creative Commons
Arianna Bresci, Koseki J. Kobayashi-Kirschvink, Giulio Cerullo

et al.

Communications Biology, Journal Year: 2024, Volume and Issue: 7(1)

Published: June 29, 2024

Accurate, rapid and non-invasive cancer cell phenotyping is a pressing concern across the life sciences, as standard immuno-chemical imaging omics require extended sample manipulation. Here we combine Raman micro-spectroscopy phase tomography to achieve label-free morpho-molecular profiling of human colon cells, following adenoma, carcinoma, metastasis disease progression, in living unperturbed conditions. We describe how decode interpret quantitative chemical co-registered morphological traits from fingerprint spectra refractive index tomograms. Our multimodal strategy rapidly distinguishes phenotypes, limiting observations low number pristine cells culture. This synergistic dataset allows us study independent or correlated information spectral tomographic maps, it benefits type inference. method valuable asset biomedical research, particularly when biological material short supply, holds potential for monitoring progression organisms.

Language: Английский

Citations

2
Coherent Raman microscopy visualizes ongoing cellular senescence through amide I peak shifts originating from β sheets in disordered nucleolar proteins DOI Creative Commons
S Ishibashi, Akihito Inoko, Yuki Oka

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: Nov. 11, 2024

Abstract Cellular senescence occurs through the accumulation of many kinds stresses. Senescent cells in tissues also cause various age-related disorders. Therefore, detecting them without labeling is beneficial for medical research and developing diagnostic methods. However, existing biomarkers have limitations requiring fixation labeling, or their molecular backgrounds are uncertain. Coherent anti-Stokes Raman scattering (CARS) spectroscopic imaging a novel option because it can assess visualize structures based on fingerprint. Here, we present new label-free method to cellular using CARS nucleoli. We found peak nucleolar amide I band shifted higher wavenumber binuclear senescent cells, which reflects changes protein secondary structure from predominant α-helices β-sheets originating amyloid-like aggregates. Following this, developed procedure that by providing ratios subtractions these two components. confirmed aggregates due unfolded/misfolded proteins produced proteasome inhibition. Finally, this help defects naïve even before binucleation. Thus, our evaluate ongoing defects.

Language: Английский

Citations

2
Label‐Free Visualization and Morphological Profiling of Neuronal Differentiation and Axonal Degeneration through Quantitative Phase Imaging DOI
Jeong Hee Kim, Aysel Cetinkaya‐Fisgin,

Noah Zahn

et al.

Advanced Biology, Journal Year: 2024, Volume and Issue: 8(5)

Published: March 28, 2024

Abstract Understanding the intricate processes of neuronal growth, degeneration, and neurotoxicity is paramount for unraveling nervous system function holds significant promise in improving patient outcomes, especially context chemotherapy‐induced peripheral neuropathy (CIPN). These are influenced by a broad range entwined events facilitated chemical, electrical, mechanical signals. The progress each process inherently linked to phenotypic changes cells. Currently, primary means demonstrating morphological rely on measurements neurite outgrowth axon length. However, conventional techniques monitoring these often require extensive preparation enable manual or semi‐automated measurements. Here, label‐free non‐invasive approach employed differentiation degeneration using quantitative phase imaging (QPI). Operating unlabeled specimens offering little no phototoxicity photobleaching, QPI delivers maps optical path length delays that provide an objective measure cellular morphology dynamics. This enables visualization quantification other physical properties dorsal root ganglion (DRG) cells, allowing greater understanding responses stimuli simulating CIPN conditions. research paves new avenues development more effective strategies clinical management neurotoxicity.

Language: Английский

Citations

1
Analysis of the X-ray induced changes in lipids extracted from hepatocarcinoma cells by means of ATR-FTIR spectroscopy DOI

Valeria Cardamuro,

Bahar Faramarzi,

Martina Moggio

et al.

Vibrational Spectroscopy, Journal Year: 2024, Volume and Issue: 132, P. 103697 - 103697

Published: May 1, 2024

Language: Английский

Citations

1