A Pump‐Free, Hydraulic‐Amplification Oscillatory Microfluidic Device for Continuous Particle and Cell Manipulation DOI Creative Commons
Yong Liu, Mingyi Liang, Shanshan Xu

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 23, 2025

Abstract Microfluidics can achieve the spatiotemporal manipulation of particles and cells in microscale fluids, but highly relies on accuracy pumping systems. To overcome this issue, a pump‐free, hydraulic‐amplification oscillatory microfluidic (PHOMF) device is presented, which be actuated by fingers to handle within microchannel. The PHOMF has module for pressure transfer soft microchannel generation flows. This made possible periodic finger‐driven liquid causes deform then drives reciprocating flow fluid volumes In flow, single‐line focusing driven spatially accumulated inertial elastic lift forces. particle elasto‐inertial theory been revealed. demonstrate system's practicality, utilized early observation platelet clots (3 min) rapid staining cancer (8 min). provides miniaturized, inexpensive, efficient detection tool lab‐on‐a‐chip, potential become mass‐produced, widely available, convenient disease product.

Language: Английский

A comprehensive review on recent advances in exosome isolation and characterization: Toward clinical applications DOI Creative Commons
Nihat Dilsiz

Translational Oncology, Journal Year: 2024, Volume and Issue: 50, P. 102121 - 102121

Published: Sept. 14, 2024

Language: Английский

Citations

54
Extracellular Vesicle Preparation and Analysis: A State‐of‐the‐Art Review DOI Creative Commons
Zesheng Wang, Xiaoyu Zhou, Qinglong Kong

et al.

Advanced Science, Journal Year: 2024, Volume and Issue: 11(30)

Published: June 14, 2024

Abstract In recent decades, research on Extracellular Vesicles (EVs) has gained prominence in the life sciences due to their critical roles both health and disease states, offering promising applications diagnosis, drug delivery, therapy. However, inherent heterogeneity complex origins pose significant challenges preparation, analysis, subsequent clinical application. This review is structured provide an overview of biogenesis, composition, various sources EVs, thereby laying groundwork for a detailed discussion contemporary techniques preparation analysis. Particular focus given state‐of‐the‐art technologies that employ microfluidic non‐microfluidic platforms EV processing. Furthermore, this discourse extends into innovative approaches incorporate artificial intelligence cutting‐edge electrochemical sensors, with particular emphasis single proposes current outlines prospective avenues future research. The objective motivate researchers innovate expand methods analysis fully unlocking biomedical potential.

Language: Английский

Citations

39
SERS biosensors for liquid biopsy towards cancer diagnosis by detection of various circulating biomarkers: current progress and perspectives DOI Creative Commons
Nana Lyu, Amin Hassanzadeh‐Barforoushi,

Laura M. Rey Gomez

et al.

Nano Convergence, Journal Year: 2024, Volume and Issue: 11(1)

Published: May 29, 2024

Abstract Liquid biopsy has emerged as a promising non-invasive strategy for cancer diagnosis, enabling the detection of various circulating biomarkers, including tumor cells (CTCs), nucleic acids (ctNAs), tumor-derived small extracellular vesicles (sEVs), and proteins. Surface-enhanced Raman scattering (SERS) biosensors have revolutionized liquid by offering sensitive specific methodologies these biomarkers. This review comprehensively examines application SERS-based identification analysis biomarkers CTCs, ctNAs, sEVs proteins in diagnosis. The discussion encompasses diverse range SERS biosensor platforms, label-free assay, magnetic bead-based microfluidic device-based system, paper-based each demonstrating unique capabilities enhancing sensitivity specificity critically assesses strengths, limitations, future directions

Language: Английский

Citations

29
Particle manipulation under X-force fields DOI
Chun‐Dong Xue,

Yifan Yin,

Xiaoyu Xu

et al.

Lab on a Chip, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

This review highlights recent technological advances for progress in particle manipulation under X-force fields, and forecasts the trajectory of future developments.

Language: Английский

Citations

2
The new advance of exosome-based liquid biopsy for cancer diagnosis DOI Creative Commons

Haozhou Tang,

Dan Yu, Jiahui Zhang

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Oct. 8, 2024

Liquid biopsy is a minimally invasive method that uses biofluid samples instead of tissue for cancer diagnosis. Exosomes are small extracellular vesicles secreted by donor cells and act as mediators intercellular communication in human health disease. Due to their important roles, exosomes have been considered promising biomarkers liquid biopsy. However, traditional methods exosome isolation cargo detection time-consuming inefficient, limiting practical application. In the past decades, many new strategies, such microfluidic chips, nanowire arrays electrochemical biosensors, proposed achieve rapid, accurate high-throughput analysis exosomes. this review, we discussed about advance exosome-based technology, including isolation, enrichment, approaches. The comparison currently available also included. Finally, summarized advantages limitations present strategies further gave perspective future translational use.

Language: Английский

Citations

9
Intranasal delivery of extracellular vesicles: A promising new approach for treating neurological and respiratory disorders DOI

Sofía V Sánchez,

Gabriela N Otavalo,

Florence Gazeau

et al.

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 379, P. 489 - 523

Published: Jan. 18, 2025

Language: Английский

Citations

1
Recent Advances in Mass Spectrometry-Based Bottom-Up Proteomics DOI
Cameron S. Movassaghi, Jie Sun, Yuming Jiang

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 25, 2025

Mass spectrometry-based proteomics is about 35 years old, and recent progress appears to be speeding up across all subfields. In this review, we focus on advances over the last two in select areas within bottom-up proteomics, including approaches high-throughput experiments, data analysis using machine learning, drug discovery, glycoproteomics, extracellular vesicle structural proteomics.

Language: Английский

Citations

1
Fully Integrated Centrifugal Microfluidics for Rapid Exosome Isolation, Glycan Analysis, and Point-of-Care Diagnosis DOI
Xudong Zhao, Xiang Liu, Tucan Chen

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 27, 2025

Exosomes present in the circulatory system demonstrate considerable promise for diagnosis and treatment of diseases. Nevertheless, complex nature blood samples prevalence highly abundant proteins pose a significant obstacle to prompt effective isolation functional evaluation exosomes from blood. Here, we fully integrated lab-on-a-disc equipped with two nanofilters, also termed iExoDisc, which facilitates automated 400 μL within 45 min. By integrating plasma separation module, protein removal nanopore membrane-based total resulting exhibited significantly increased purity (∼3-6-fold) compared conventional ultracentrifugation polymer precipitation. Additionally, then successfully performed nontargeted targeted glycan profiling on derived clinical triple-negative breast cancer (TNBC) patients using MALDI-TOF-MS lectin microarray containing 56 kinds lectins. The findings both methodologies indicated that galactosylation sialylation exhibit potential as diagnostic indicators TNBC. Finally, by utilizing exosome-specific glycosylated CD63 proof-of-concept, realized integration point-of-care on-chip exosome situ detection 2 h. Thus, iExoDisc provides approach early detection, liquid biopsy, diagnosis.

Language: Английский

Citations

1
Integrated Microfluidic Chip for Neutrophil Extracellular Vesicle Analysis and Gastric Cancer Diagnosis DOI Creative Commons
Dan Yu, Jianmei Gu, Jiahui Zhang

et al.

ACS Nano, Journal Year: 2025, Volume and Issue: unknown

Published: March 9, 2025

Neutrophil-derived extracellular vesicles (NEVs) are critically involved in disease progression and considered potential biomarkers. However, the tedious processes of NEV separation detection restrain their use. Herein, we presented an integrated microfluidic chip for (IMCN) analysis, which achieved immune-separation CD66b+ NEVs multiplexed contained miRNAs (termed signatures) by using 10 μL serum samples. The optimized microchannel flow rate IMCN enabled efficient capture (>90%). After recognition captured a specific CD63 aptamer, on-chip rolling circle amplification (RCA) reaction was triggered released aptamers from heat-lysed NEVs. Then, RCA products bound to molecular beacons (MBs), initiating allosteric hairpin structures amplified "turn on" fluorescence signals (RCA-MB assay). Clinical sample analysis showed that signatures had high area under curve (AUC) distinguishing between healthy control (HC) gastric cancer (GC) (0.891), benign diseases (BGD) GC (0.857). Notably, AUC reached 0.912 with combination five biomarkers (NEV signatures, CEA, CA199) differentiate HC, diagnostic accuracy further increased machine learning (ML)-based ensemble classification system. Therefore, developed is valuable platform may have use diagnosis.

Language: Английский

Citations

1
Oscillating microbubble array–based metamaterials (OMAMs) for rapid isolation of high-purity exosomes DOI Creative Commons
Xinjia Li,

Zhiting Deng,

Wenjun Zhang

et al.

Science Advances, Journal Year: 2025, Volume and Issue: 11(16)

Published: April 16, 2025

Exosomes secreted by cells hold substantial potential for disease diagnosis and treatment. However, the rapid isolation of high-purity exosomes their subpopulations from biofluids (e.g., undiluted whole blood) remains challenging. This study presents oscillating microbubble array–based metamaterials (OMAMs) enabling without labeling or preprocessing. Particularly, leveraging acoustically excited oscillation, OMAMs can generate numerous acoustofluidic traps filtering in-fluid micro/nanoparticles, thus allowing removing bioparticles larger than to obtain (93%) blood in ~3 minutes. Moreover, exosome different size ranges be isolated tuning oscillation amplitude. Additionally, as each functions an ultradeep subwavelength (~λ/186) acoustic amplifier a nonlinear source, high-resolution complex energy patterns tune activating different-sized microbubbles at distinct resonance frequencies.

Language: Английский

Citations

1