Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: Oct. 28, 2024
Exploring
microorganisms
with
downstream
synthetic
advantages
in
lignin
valorization
is
an
effective
strategy
to
increase
target
product
diversity
and
yield.
This
study
ingeniously
engineers
the
non-lignin-degrading
bacterium
Ralstonia
eutropha
H16
(also
known
as
Cupriavidus
necator
H16)
convert
lignin,
a
typically
underutilized
by-product
of
biorefinery,
into
valuable
bioplastic
polyhydroxybutyrate
(PHB).
The
aromatic
metabolism
capacities
R.
for
different
lignin-derived
aromatics
(LDAs)
are
systematically
characterized
complemented
by
integrating
robust
functional
modules
including
O-demethylation,
aldehyde
mitigation
inhibition.
A
pivotal
discovery
regulatory
element
PcaQ,
which
highly
responsive
hub
metabolite
protocatechuic
acid
during
degradation.
Based
on
computer-aided
design
we
develop
metabolite-based
autoregulation
(HMA)
system.
system
can
control
genes
expression
response
heterologous
LDAs
enhance
efficiency.
Multi-module
genome
integration
directed
evolution
further
fortify
strain's
stability
conversion
capacities,
leading
PHB
production
titer
2.38
g/L
using
sole
carbon
source.
work
not
only
marks
leap
from
components
but
also
provides
redesign
non-LDAs-degrading
microbes
efficient
valorization.
One
challenge
that
possessing
metabolic
unable
lignin.
Here,
authors
engineer
efficiently
self-enhanced
Microorganisms,
Journal Year:
2025,
Volume and Issue:
13(4), P. 935 - 935
Published: April 18, 2025
Efficient
utilization
of
lignin,
a
complex
polymer
in
plant
cell
walls,
is
one
the
key
strategies
for
developing
green
and
sustainable
bioeconomy.
However,
bioconversion
lignin
poses
significant
challenge
due
to
its
recalcitrant
nature.
Microorganisms,
particularly
fungi
bacteria,
play
crucial
role
biodegradation,
using
various
enzymatic
pathways.
Among
Pseudomonas
putida
considered
promising
host
degradation
valorization,
robust
flexible
metabolism
tolerance
many
noxious
toxic
compounds.
This
review
explores
mechanisms
breakdown
by
microorganisms,
with
focus
on
P.
putida’s
metabolic
versatility
genetic
engineering
potential.
By
leveraging
advanced
tools
pathway
optimization,
can
be
engineered
efficiently
convert
into
valuable
bioproducts,
offering
solutions
valorization
industrial
applications.
Applied and Environmental Microbiology,
Journal Year:
2024,
Volume and Issue:
90(7)
Published: June 28, 2024
Biological
valorization
of
lignin,
the
second
most
abundant
biopolymer
on
Earth,
is
an
indispensable
sector
to
build
a
circular
economy
and
net-zero
future.
However,
lignin
recalcitrant
bioupcycling,
demanding
innovative
solutions.
We
report
here
biological
lignin-derived
aromatic
carbon
value-added
chemicals
without
requesting
extra
organic
freshwater
via
reprogramming
marine
Angewandte Chemie,
Journal Year:
2024,
Volume and Issue:
136(48)
Published: Sept. 17, 2024
Abstract
The
natural
heterogeneity
of
guaiacyl
(G)
and
syringyl
(S)
compounds
resulting
from
lignin
processing
hampers
their
direct
use
as
plant‐based
chemicals
materials.
Herein,
we
explore
six
short
polyphenol
oxidases
(PPOs)
lignocellulose‐degrading
ascomycetes
for
capacity
to
react
with
G‐type
S‐type
phenolic
compounds.
All
PPOs
catalyze
the
ortho‐
hydroxylation
(guaiacol,
vanillic
acid,
ferulic
acid),
forming
corresponding
methoxy‐
ortho
‐diphenols.
Remarkably,
a
subset
these
is
also
active
towards
S‐compounds
(syringol,
syringic
sinapic
acid)
in
identical
diphenols.
Assays
18
O
2
demonstrate
that
particular
‐hydroxylation
‐demethoxylation
generate
methanol
co‐product.
Oxidative
(
−)
demethoxylation
novel
reaction
PPOs,
which
propose
occurs
by
distinct
mechanism
compared
aryl‐O‐demethylases.
We
further
show
addition
reducing
agent
can
steer
PPO
form
‐diphenols
both
G‐
substrates
rather
than
reactive
quinones
lead
unfavorable
polymerization.
Application
opens
new
routes
reduce
methoxylation
degree
mixtures
G
S
lignin‐derived
