Chemical Engineering Journal, Journal Year: 2024, Volume and Issue: unknown, P. 158773 - 158773
Published: Dec. 1, 2024
Language: Английский
Materials Today Bio, Journal Year: 2025, Volume and Issue: 31, P. 101536 - 101536
Published: Feb. 3, 2025
Language: Английский
Citations
1
Science Advances, Journal Year: 2024, Volume and Issue: 10(47)
Published: Nov. 22, 2024
Closed-loop strategies offer advanced therapeutic potential through intelligent disease management. Here, we develop a hydrogel-based, single-component, organic electronic device for closed-loop neurotherapy. Fabricated out of conductive hydrogels, the consists flexible array microneedle electrodes, each which can be individually addressed to perform electrical recording and control chemical release with sophisticated spatiotemporal control, thus pioneering smart antiseizure system by combining pharmacological interventions. The recorded neural signal acts as trigger voltage-driven drug in detected pathological conditions predicted real-time electrophysiology analysis. When implanted into epileptic animals, enables autonomous management, where dosing antiepileptic is controlled time-sensitive, region-selective, dose-adaptive manner, allowing inhibition seizure outbursts delivery just-necessary dosages. side effects are minimized dosages three orders magnitude lower than usage approaches simulating existing clinical treatments.
Language: Английский
Citations
5
Nature Communications, Journal Year: 2025, Volume and Issue: 16(1)
Published: Jan. 28, 2025
Creating durable, motion-compliant neural interfaces is crucial for accessing dynamic tissues under in vivo conditions and linking activity with behaviors. Utilizing the self-alignment of nano-fillers a polymeric matrix repetitive tension, here, we introduce conductive carbon nanotubes high aspect ratios into semi-crystalline polyvinyl alcohol hydrogels, create electrically anisotropic percolation pathways through cyclic stretching. The resulting hydrogel fibers (diameter 187 ± 13 µm) exhibit fatigue resistance (up to 20,000 cycles at 20% strain) stretchability 64.5 7.9% low electrochemical impedance (33.20 9.27 kΩ @ 1 kHz cm length). We observe reconstructed nanofillers' axial alignment corresponding decrease along direction fabricate fiber-shaped hydrogels bioelectronic devices implant them wild-type transgenic Thy1::ChR2-EYFP mice record electromyographic signals from muscles anesthetized freely moving conditions. These effectively enable simultaneous recording electrical ventral spinal cord neurons tibialis anterior during optogenetic stimulation. Importantly, maintain functionality intraspinal electrophysiology recordings over eight months after implantation, demonstrating their durability potential long-term monitoring neurophysiological studies. essential Here, authors nanotube-enhanced creating anisotropic, fatigue-resistant electrophysiological mice.
Language: Английский
Citations
0
Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)
Published: Feb. 20, 2025
spinal cord injury (SCI) disrupts the gut microbiota, worsening injury's impact. Fecal microbiota transplantation (FMT) is increasingly recognized as a promising strategy to improve neural function post-SCI, yet its precise mechanisms are still far from clear. The present study aims elucidate how FMT influences motor recovery and underlying utilizing SCI mouse model. Mice with received healthy donors. We used 16 S rRNA amplicon sequencing analyze alterations of microbes. Pathological in tissue, including neuronal survival, axonal regeneration, cell proliferation, neuroinflammation, were assessed among experimental groups. Additionally, RNA (RNA-seq) was explore relevant signaling pathways. Significant shifts composition following observed through analysis. On day 7 group exhibited significantly higher diversity compared ABX group, more closely resembling that mice. promoted survival leading notable improvements control Immunofluorescence staining showed increased alleviated extracellular matrix (ECM) deposition, diminished glial scar formation, reduced inflammation FMT-treated RNA-seq analysis indicated induced transcriptomic changes associated material metabolism, ECM remodeling, anti-inflammatory responses. restored balance mice, mitigated inflammation, establishing an optimal environment for recovery. These findings demonstrated may represent valuable approach enhance functional SCI.
Language: Английский
Citations
0
Jentashapir Journal of Cellular and Molecular Biology, Journal Year: 2025, Volume and Issue: 16(1)
Published: Feb. 13, 2025
Context: Recent advances in induced pluripotent stem cells (iPSCs), CRISPR-Cas9 gene editing, nanotechnologies, and artificial intelligence have revolutionized regenerative medicine (RM) as a transformative field for tackling difficult medical problems. These breakthroughs promise specific treatments, proper restoration of tissue function, substantial improvements the quality life patients whose ailments cannot yet be cured. This review explores cutting-edge advancements RM platforms such cell therapy, 3D bioprinting, nanotechnology. The study also aims to shed light on challenges clinical translation policy implications, which are crucial fostering sustainable progressive discipline. Evidence Acquisition: manuscript draws research development application technologies. It synthesizes data cells, engineering, vitro organoid industry, (AI), nanotechnology that illustrate therapeutic potential. identify ethical, regulatory, practical hurdles translating from practice. Results: Breakthroughs those iPSC-derived organoids, nanostructured materials exhibit significant preclinical settings. Platforms organ-on-chip AI tools further enhance drug discovery treatment monitoring, while biomaterials scaffold-based approaches repair regeneration. Nevertheless, despite these advances, persist regarding scale-up, safety, ethical considerations. Conclusions: Innovations represent paradigm shift purely symptomatic treatments restorative therapies. Successful integration into practice will require multidisciplinary collaborative work, imposition rigorous safety protocols, enabling regulatory frameworks. Addressing would enable realize its true potential foundation 21st-century healthcare.
Language: Английский
Citations
0
Cell Reports Physical Science, Journal Year: 2025, Volume and Issue: unknown, P. 102498 - 102498
Published: March 1, 2025
Language: Английский
Citations
0
Composites Part B Engineering, Journal Year: 2025, Volume and Issue: unknown, P. 112383 - 112383
Published: March 1, 2025
Language: Английский
Citations
0
ACS Nano, Journal Year: 2025, Volume and Issue: unknown
Published: April 10, 2025
Skin burn wounds (SBWs) are common clinical injuries due to excessive exposure factors including heat, radiation, chemical agents, etc. However, the efficient healing of SBWs is still challenging persistent inflammation and high risk local infection. To meet these challenges, we report a hydrogel-based bioactive synthetic skin (HBSS) from biocompatible components as dressing materials for wound treatment, which mediated localized H2S release stimulate tissue regeneration while preventing bacterial infection inflammation. Here, donor (N-(benzoyl mercapto) benzamide) was first coassembled with thioketal (TK)-ligated dopamine dimer form nanoscale assemblies (DDNs), were then integrated into Schiff base-cross-linked hyaluronic acid-carboxymethyl chitosan hydrogels. The elevated acidity in would trigger hydrogel degradation DDNs, further activated by ROS-induced cleavage TK linkers gas attenuating ROS stress self-immolative manner, thus promoting angiogenesis through activating AMPK RAS-MAPK-AP1 prohealing pathways, enabling M1-to-M2 macrophage reprogramming ERK1/2 NRF2 signaling. Meanwhile, network could inhibit colonization at site prevent These merits acted cooperative manner enable accelerated robust healing, offering an approach treatment clinic.
Language: Английский
Citations
0
Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 162988 - 162988
Published: April 1, 2025
Language: Английский
Citations
0
Advanced Materials, Journal Year: 2025, Volume and Issue: unknown
Published: April 29, 2025
Abstract Spinal cord injury (SCI) is a debilitating condition that leads to severe disabilities and imposes significant economic social burdens. Current therapeutic strategies primarily focus on symptom management, with limited success in promoting full neurological recovery. In response this challenge, the design of novel guidance conduits incorporating multiple gradient cues, inspired reported by biological processes, enhance spinal repair. These are fabricated using electrospinning masked coaxial electrospraying, simple yet effective method integrates topological, haptotactic, chemotactic cues into single scaffold. The synergy these significantly promoted cell migration, neural stem differentiation neurons, axonal extension, resulting substantial improvements regeneration functional recovery rat model. Single‐nucleus RNA sequencing further demonstrated conduit inhibited fibroblast proliferation, preserved microglial homeostasis, restored cellular proportions, facilitated neuronal axons, dendrites, synapses. This work presents an innovative, versatile platform for fabricating tissue scaffolds integrate offering promising strategy SCI treatment broader applications.
Language: Английский
Citations
0