Hepatic stellate cells: balancing homeostasis, hepatoprotection and fibrogenesis in health and disease DOI
Robert F. Schwabe, David A. Brenner

Nature Reviews Gastroenterology & Hepatology, Journal Year: 2025, Volume and Issue: unknown

Published: May 22, 2025

Language: Английский

Antifibrotic Therapies for Metabolic Dysfunction-associated Steatotic Liver Disease DOI Creative Commons
Robert F. Schwabe, Frank Tacke, Atsushi Sugimoto

et al.

JHEP Reports, Journal Year: 2025, Volume and Issue: unknown, P. 101421 - 101421

Published: April 1, 2025

Language: Английский

Citations

1
Building an atlas of mechanobiology: high-throughput contractility screen of 2418 kinase inhibitors in five primary human cell types reveals selective divergent responses among related cell types DOI Creative Commons

Anton Shpak,

Jeremy Wan,

Ricky Huang

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Abstract Cellular mechanical forces play crucial roles in both normal physiology and disease, yet drug discovery efforts targeting mechanobiology have been limited part by assumptions about the conservation of contractile pathways across cell types. Here, we present first high-throughput contractility screen an annotated kinase inhibitor library, evaluating 2,418 compounds five primary human types using FLECS (Fluorescent Elastomer Contractility Sensors) platform. Quantification responses revealed selective divergent among related Clustering analysis identified distinct mechanobiological profiles novel pathway associations that challenge assumption are too highly conserved for targeting. This systematic approach supports wider adoption phenotypic screening as a viable strategy discovering cell-type specific modulators broad range mechanically-driven disease indications.

Language: Английский

Citations

0
TRPV4 activation in Schwann cells mediates mechanically induced pain of oral cancer DOI Creative Commons
Yatendra Mulpuri, Nguyen Huu Tu, Kenji Inoue

et al.

Frontiers in Pain Research, Journal Year: 2025, Volume and Issue: 6

Published: March 12, 2025

Introduction Patients with oral cancer often experience intense functional pain due to mechanical stimulation at the site. The role of mechanosensitive ion channels in pain, such as TRPV4, is not fully understood. Objectives Our objective was investigate Schwann cell TRPV4 pain. Methods We examined impact inhibition on NU/J and C57BL/6J mice injected human tongue line (HSC-3) mouse (MOC2) hind paw or tongue. Mechanical heat sensitivity were assessed using von Frey Hargreaves tests, respectively. expression activity cells analyzed immunohistochemistry, qRT-PCR, Ca 2+ imaging, patch-clamp electrophysiology. effect activation responses evaluated a piezo stimulator. Conditioned media (CM) from TRPV4-activated into evaluate contribution hypersensitivity. Results reduced nociception dose-dependently without affecting sensitivity. also decreased facial mice. expressed mainly plasma membrane induced whole-cell currents cells. Mechanoactivated inhibited by antagonist HC-067047. CM hypersensitivity mice, which blocked pre-treatment Conclusion plays mediating mechanically cancer.

Language: Английский

Citations

0
M2 macrophage co-culture overrides viscoelastic hydrogel mechanics to promote IL-6-dependent fibroblast activation DOI Creative Commons

Leilani R. Astrab,

Mackenzie L. Skelton,

Steven R. Caliari

et al.

Published: April 1, 2025

Language: Английский

Citations

0
Fibroproliferative conditions: the 3R approach bridging fibrosis and tumors DOI
Chenyu Huang,

Yue Shao,

Jianbo Bai

et al.

Trends in Molecular Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Effects of embryonic origin, tissue cues and pathological signals on fibroblast diversity in humans DOI
Marta Torregrossa, Lindsay C. Davies, Hans‐Günther Machens

et al.

Nature Cell Biology, Journal Year: 2025, Volume and Issue: unknown

Published: April 22, 2025

Language: Английский

Citations

0
Integration of Mechanics and Immunology: Perspective for Understanding Fibrotic Disease Mechanisms and Innovating Therapeutic Strategies DOI
Lei Min, Guobao Chen

Acta Biomaterialia, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0
Signals from the extracellular matrix: Region- and sex-specificity in cardiac aging DOI Creative Commons

Md. Faris H. Ramli,

Brian A. Aguado, Jennifer L. Young

et al.

Current Opinion in Cell Biology, Journal Year: 2025, Volume and Issue: 95, P. 102524 - 102524

Published: May 9, 2025

During aging, the cardiac extracellular matrix (ECM) undergoes gradual remodeling that reduces heart's ability to function. Specific ECM changes cause alterations in cellular signaling pathways, eliciting maladaptive responses. Here, we provide insight into current knowledge of how age-specific contribute altered ligand-receptor interactions, dysregulated mechanotransduction, and propagation pro-fibrotic cascades underpin dysfunction. We also highlight regional sex differences new biomolecular bioengineered technologies have recently uncovered. call for biomaterial strategies mimic spatiotemporal sex-specific equip researchers with tools unravel complex events. believe this can be achieved through interdisciplinary cooperation amongst spanning biology, biomaterials, spatial omics, biomedical engineering.

Language: Английский

Citations

0
Exploring therapy for tooth eruption disturbances from the perspective of ECM-macrophage DOI

Han Qin,

Jun Cai

Medical Hypotheses, Journal Year: 2025, Volume and Issue: unknown, P. 111662 - 111662

Published: May 1, 2025

Language: Английский

Citations

0
Mechanical mechanics-reclaiming a new battlefield for chronic liver disease DOI Creative Commons

Y Zhang,

Tianle Ma,

XingXing Lu

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: May 1, 2025

Language: Английский

Citations

0