Genetically encoded indicators of neuronal activity

Nature Neuroscience, Journal Year: 2016, Volume and Issue: 19(9), P. 1142 - 1153

Published: Aug. 26, 2016

Language: Английский

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The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

Trends in Biochemical Sciences, Journal Year: 2016, Volume and Issue: 42(2), P. 111 - 129

Published: Nov. 1, 2016

Language: Английский

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Whole-brain activity mapping onto a zebrafish brain atlas

Nature Methods, Journal Year: 2015, Volume and Issue: 12(11), P. 1039 - 1046

Published: Sept. 14, 2015

Language: Английский

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Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks

Chemical Reviews, Journal Year: 2018, Volume and Issue: 118(24), P. 11707 - 11794

Published: Dec. 14, 2018

Cellular signaling networks are the foundation which determines fate and function of cells as they respond to various cues stimuli. The discovery fluorescent proteins over 25 years ago enabled development a diverse array genetically encodable biosensors that capable measuring spatiotemporal dynamics signal transduction pathways in live cells. In an effort encapsulate breadth have expanded, we endeavored assemble comprehensive list published engineered biosensors, discuss many molecular designs utilized their development. Then, review how high temporal spatial resolution afforded by has aided our understanding regulation at cellular subcellular level. Finally, highlight some emerging areas research both biosensor design applications on forefront

Language: Английский

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Genetic Dissection of Neural Circuits: A Decade of Progress

Neuron, Journal Year: 2018, Volume and Issue: 98(2), P. 256 - 281

Published: April 1, 2018

Tremendous progress has been made since Neuron published our Primer on genetic dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical, neurophysiological, and perturbation studies have carried out in a multitude invertebrate vertebrate organisms, linking neurons to behavioral functions. New methods allow systematic classification cell types provide access diverse neuronal for connectivity coding during behavior. Here we evaluate key advances over the past decade discuss future directions.

Language: Английский

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Cortical column and whole-brain imaging with molecular contrast and nanoscale resolution

Science, Journal Year: 2019, Volume and Issue: 363(6424)

Published: Jan. 18, 2019

Optical and electron microscopy have made tremendous inroads toward understanding the complexity of brain. However, optical offers insufficient resolution to reveal subcellular details, lacks throughput molecular contrast visualize specific constituents over millimeter-scale or larger dimensions. We combined expansion lattice light-sheet image nanoscale spatial relationships between proteins across thickness mouse cortex entire Drosophila These included synaptic at dendritic spines, myelination along axons, presynaptic densities dopaminergic neurons in every fly brain region. The technology should enable statistically rich, large-scale studies neural development, sexual dimorphism, degree stereotypy, structural correlations behavior activity, all with contrast.

Language: Английский

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DNA methylation changes in plasticity genes accompany the formation and maintenance of memory

Nature Neuroscience, Journal Year: 2015, Volume and Issue: 19(1), P. 102 - 110

Published: Dec. 14, 2015

Language: Английский

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Systemic AAV vectors for widespread and targeted gene delivery in rodents

Nature Protocols, Journal Year: 2019, Volume and Issue: 14(2), P. 379 - 414

Published: Jan. 8, 2019

Language: Английский

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Zebrafish Larvae as a Behavioral Model in Neuropharmacology

Biomedicines, Journal Year: 2019, Volume and Issue: 7(1), P. 23 - 23

Published: March 26, 2019

Zebrafish larvae show a clear and distinct pattern of swimming in response to light dark conditions, following the development swim bladder at 4 days post fertilization. This behavior is increasingly employed screening neuroactive drugs. The recent emergence high-throughput techniques for automatic tracking zebrafish has further allowed an objective efficient way finding subtle behavioral changes that could go unnoticed during manual observations. review highlights use as model compounds. We describe, brief, repertoire larvae. Then, we focus on utilization light-dark locomotion test identifying

Language: Английский

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Humidity Sensing in Drosophila

Current Biology, Journal Year: 2016, Volume and Issue: 26(10), P. 1352 - 1358

Published: May 1, 2016

Language: Английский

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