Integration of optogenetics with complementary methodologies in systems neuroscience

Nature reviews. Neuroscience, Journal Year: 2017, Volume and Issue: 18(4), P. 222 - 235

Published: March 17, 2017

Language: Английский

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Genetically encoded indicators of neuronal activity

Nature Neuroscience, Journal Year: 2016, Volume and Issue: 19(9), P. 1142 - 1153

Published: Aug. 26, 2016

Language: Английский

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The Growing and Glowing Toolbox of Fluorescent and Photoactive Proteins

Trends in Biochemical Sciences, Journal Year: 2016, Volume and Issue: 42(2), P. 111 - 129

Published: Nov. 1, 2016

Language: Английский

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Multifunctional materials for implantable and wearable photonic healthcare devices

Nature Reviews Materials, Journal Year: 2020, Volume and Issue: 5(2), P. 149 - 165

Published: Jan. 7, 2020

Language: Английский

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Optical magnetic detection of single-neuron action potentials using quantum defects in diamond

Proceedings of the National Academy of Sciences, Journal Year: 2016, Volume and Issue: 113(49), P. 14133 - 14138

Published: Nov. 22, 2016

Significance We demonstrate noninvasive detection of action potentials with single-neuron sensitivity, including in whole organisms. Our sensor is composed quantum defects within a diamond chip, which detect time-varying magnetic fields generated by potentials. The biocompatible and can be brought into close proximity to the organism without adverse effect, allowing for long-term observation superior resolution neuron fields. Optical also provides information about potential propagation that not easily available existing methods. technique requires no labeling or genetic modification, allows submillisecond time resolution, does bleach, senses through opaque tissue. With further development, we expect micrometer-scale imaging variety neuronal phenomena.

Language: Английский

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Fast high-resolution miniature two-photon microscopy for brain imaging in freely behaving mice

Nature Methods, Journal Year: 2017, Volume and Issue: 14(7), P. 713 - 719

Published: May 29, 2017

Language: Английский

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Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks

Chemical Reviews, Journal Year: 2018, Volume and Issue: 118(24), P. 11707 - 11794

Published: Dec. 14, 2018

Cellular signaling networks are the foundation which determines fate and function of cells as they respond to various cues stimuli. The discovery fluorescent proteins over 25 years ago enabled development a diverse array genetically encodable biosensors that capable measuring spatiotemporal dynamics signal transduction pathways in live cells. In an effort encapsulate breadth have expanded, we endeavored assemble comprehensive list published engineered biosensors, discuss many molecular designs utilized their development. Then, review how high temporal spatial resolution afforded by has aided our understanding regulation at cellular subcellular level. Finally, highlight some emerging areas research both biosensor design applications on forefront

Language: Английский

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Genetic Dissection of Neural Circuits: A Decade of Progress

Neuron, Journal Year: 2018, Volume and Issue: 98(2), P. 256 - 281

Published: April 1, 2018

Tremendous progress has been made since Neuron published our Primer on genetic dissection of neural circuits 10 years ago. Since then, cell-type-specific anatomical, neurophysiological, and perturbation studies have carried out in a multitude invertebrate vertebrate organisms, linking neurons to behavioral functions. New methods allow systematic classification cell types provide access diverse neuronal for connectivity coding during behavior. Here we evaluate key advances over the past decade discuss future directions.

Language: Английский

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Bright and photostable chemigenetic indicators for extended in vivo voltage imaging

Science, Journal Year: 2019, Volume and Issue: 365(6454), P. 699 - 704

Published: Aug. 1, 2019

Genetically encoded voltage indicators (GEVIs) enable monitoring of neuronal activity at high spatial and temporal resolution. However, the utility existing GEVIs has been limited by brightness photostability fluorescent proteins rhodopsins. We engineered a GEVI, called Voltron, that uses bright photostable synthetic dyes instead protein-based fluorophores, thereby extending number neurons imaged simultaneously in vivo factor 10 enabling imaging for significantly longer durations relative to GEVIs. used Voltron mice, zebrafish, fruit flies. In mouse cortex, allowed single-trial recording spikes subthreshold signals from dozens over 15-minute period continuous imaging. larval enabled precise correlation spike timing with behavior.

Language: Английский

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A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters

Nature Chemical Biology, Journal Year: 2018, Volume and Issue: 14(4), P. 352 - 360

Published: Feb. 26, 2018

Language: Английский

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