Ultrafast neuronal imaging of dopamine dynamics with designed genetically encoded sensors

Science, Journal Year: 2018, Volume and Issue: 360(6396)

Published: May 31, 2018

Imaging dopamine release in the brain Neuromodulator alters function of target circuits poorly known ways. An essential step to address this knowledge gap is measure dynamics neuromodulatory signals while simultaneously manipulating elements circuit during behavior. Patriarchi et al. developed fluorescent protein–based indicators visualize spatial and temporal directly with high fidelity resolution. In cortex, two-photon imaging these was used map activity at cellular Science , issue p. eaat4422

Language: Английский

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CaImAn an open source tool for scalable calcium imaging data analysis

eLife, Journal Year: 2019, Volume and Issue: 8

Published: Jan. 14, 2019

Advances in fluorescence microscopy enable monitoring larger brain areas in-vivo with finer time resolution. The resulting data rates require reproducible analysis pipelines that are reliable, fully automated, and scalable to datasets generated over the course of months. We present CaImAn, an open-source library for calcium imaging analysis. CaImAn provides automatic methods address problems common pre-processing, including motion correction, neural activity identification, registration across different sessions collection. It does this while requiring minimal user intervention, good scalability on computers ranging from laptops high-performance computing clusters. is suitable two-photon one-photon imaging, also enables real-time streaming data. To benchmark performance we collected combined a corpus manual annotations multiple labelers nine mouse datasets. demonstrate achieves near-human detecting locations active neurons.

Language: Английский

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Intelligent Image-Activated Cell Sorting

Cell, Journal Year: 2018, Volume and Issue: 175(1), P. 266 - 276.e13

Published: Aug. 27, 2018

Language: Английский

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Genetically Encoded Fluorescent Biosensors Illuminate the Spatiotemporal Regulation of Signaling Networks

Chemical Reviews, Journal Year: 2018, Volume and Issue: 118(24), P. 11707 - 11794

Published: Dec. 14, 2018

Cellular signaling networks are the foundation which determines fate and function of cells as they respond to various cues stimuli. The discovery fluorescent proteins over 25 years ago enabled development a diverse array genetically encodable biosensors that capable measuring spatiotemporal dynamics signal transduction pathways in live cells. In an effort encapsulate breadth have expanded, we endeavored assemble comprehensive list published engineered biosensors, discuss many molecular designs utilized their development. Then, review how high temporal spatial resolution afforded by has aided our understanding regulation at cellular subcellular level. Finally, highlight some emerging areas research both biosensor design applications on forefront

Language: Английский

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Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice

Cell, Journal Year: 2019, Volume and Issue: 179(7), P. 1590 - 1608.e23

Published: Dec. 1, 2019

Language: Английский

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Optogenetics for light control of biological systems

Nature Reviews Methods Primers, Journal Year: 2022, Volume and Issue: 2(1)

Published: July 21, 2022

Language: Английский

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Wireless and battery-free technologies for neuroengineering

Nature Biomedical Engineering, Journal Year: 2021, Volume and Issue: 7(4), P. 405 - 423

Published: March 8, 2021

Language: Английский

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Population imaging of neural activity in awake behaving mice

Nature, Journal Year: 2019, Volume and Issue: 574(7778), P. 413 - 417

Published: Oct. 9, 2019

Language: Английский

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Circuit Investigations With Open-Source Miniaturized Microscopes: Past, Present and Future

Frontiers in Cellular Neuroscience, Journal Year: 2019, Volume and Issue: 13

Published: April 5, 2019

The ability to simultaneously image the spatiotemporal activity signatures from many neurons during unrestrained vertebrate behaviors has become possible through development of miniaturized fluorescence microscopes, or miniscopes, sufficiently light be carried by small animals such as bats, birds and rodents. Miniscopes have permitted study circuits underlying song vocalization, action sequencing, head-direction tuning, spatial memory encoding sleep name a few. foundation for these microscopes been laid over last two decades academic research with some this work resulting in commercialization. More recently, open-source initiatives led an even broader adoption miniscopes neuroscience community. Open-source designs allow rapid modification extension their function, which resulted new generation that now permit wire-free wireless recording, concurrent electrophysiology imaging, two-color detection, simultaneous optical actuation read-out well wide-field volumetric light-field imaging. These novel will further expand toolset those seeking affordable methods probe neural circuit function naturalistic behaviors. Here, we discuss early development, present use future potential miniscopes.

Language: Английский

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A genetically encoded near-infrared fluorescent calcium ion indicator

Nature Methods, Journal Year: 2019, Volume and Issue: 16(2), P. 171 - 174

Published: Jan. 21, 2019

Language: Английский

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