Cell, Journal Year: 2018, Volume and Issue: 174(4), P. 953 - 967.e22
Published: July 19, 2018
Language: Английский
Nature Cell Biology, Journal Year: 2021, Volume and Issue: 23(1), P. 11 - 22
Published: Jan. 1, 2021
Language: Английский
Citations
353
Trends in Genetics, Journal Year: 2017, Volume and Issue: 33(8), P. 540 - 552
Published: June 17, 2017
Language: Английский
Citations
321
ACS Chemical Biology, Journal Year: 2017, Volume and Issue: 13(2), P. 406 - 416
Published: Oct. 16, 2017
Next-generation DNA sequencing technologies have led to a massive accumulation of genomic and transcriptomic data from patients healthy individuals. The major challenge ahead is understand the functional significance elements human genome transcriptome, implications for diagnosis treatment. Genetic screens in mammalian cells are powerful approach systematically elucidating gene function health disease states. In particular, recently developed CRISPR/Cas9-based screening approaches enormous potential uncover mechanisms therapeutic strategies diseases. focus this review use CRISPR interference (CRISPRi) activation (CRISPRa) genetic cells. We introduce underlying technology present different types CRISPRi/a screens, including those based on cell survival/proliferation, sensitivity drugs or toxins, fluorescent reporters, single-cell transcriptomes. Combinatorial which large numbers pairs targeted construct interaction maps, reveal pathway relationships protein complexes. compare contrast CRISPRi CRISPRa with alternative technologies, RNA (RNAi) nuclease-based screens. Finally, we highlight challenges opportunities ahead.
Language: Английский
Citations
313
Nature reviews. Cancer, Journal Year: 2022, Volume and Issue: 22(5), P. 259 - 279
Published: Feb. 22, 2022
Language: Английский
Citations
291
Cell, Journal Year: 2018, Volume and Issue: 174(4), P. 953 - 967.e22
Published: July 19, 2018
Language: Английский
Citations
287