Decoding internally generated transitions of conscious contents in the prefrontal cortex without subjective reports DOI Creative Commons
Vishal Kapoor, Abhilash Dwarakanath, Shervin Safavi

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: March 22, 2022

Abstract A major debate about the neural correlates of conscious perception concerns its cortical organization, namely, whether it includes prefrontal cortex (PFC), which mediates executive functions, or is constrained within posterior cortices. It has been suggested that PFC activity during paradigms investigating conflated with post-perceptual processes associated reporting contents consciousness feedforward signals originating from exogenous stimulus manipulations and relayed via areas. We addressed this by simultaneously probing neuronal populations in rhesus macaque (Macaca mulatta) a no-report paradigm, capable instigating internally generated transitions perception, without changes visual stimulation. find feature-selective neurons are modulated concomitantly subjective perceptual suppression their preferred both externally induced perception. Importantly, enables reliable single-trial, population decoding contents. Control experiments confirm significant contents, even when oculomotor responses, used for inferring suppressed. These findings suggest reliably reflected absence volitional reports sensory input.

Language: Английский

Inhibitory stabilization and cortical computation DOI
Sadra Sadeh, Claudia Clopath

Nature reviews. Neuroscience, Journal Year: 2020, Volume and Issue: 22(1), P. 21 - 37

Published: Nov. 11, 2020

Language: Английский

Citations

162
Sono-optogenetics facilitated by a circulation-delivered rechargeable light source for minimally invasive optogenetics DOI Creative Commons
Xiang Wu, Xingjun Zhu, P. Andrew Chong

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2019, Volume and Issue: 116(52), P. 26332 - 26342

Published: Dec. 6, 2019

Optogenetics, which uses visible light to control the cells genetically modified with light-gated ion channels, is a powerful tool for precise deconstruction of neural circuitry neuron-subtype specificity. However, due limited tissue penetration light, invasive craniotomy and intracranial implantation tethered optical fibers are usually required in vivo optogenetic modulation. Here we report mechanoluminescent nanoparticles that can act as local sources brain when triggered by brain-penetrant focused ultrasound (FUS) through intact scalp skull. Mechanoluminescent be delivered into blood circulation via i.v. injection, recharged 400-nm photoexcitation superficial vessels during circulation, turned on FUS emit 470-nm repetitively stimulation. Unlike conventional "outside-in" approaches optogenetics fiber implantation, our method provides an "inside-out" approach deliver nanoscopic emitters intrinsic circulatory system switch them off at any time location interest without extravasation minimally interface.

Language: Английский

Citations

158
The structures and functions of correlations in neural population codes DOI
Stefano Panzeri, Monica Moroni, Houman Safaai

et al.

Nature reviews. Neuroscience, Journal Year: 2022, Volume and Issue: 23(9), P. 551 - 567

Published: June 22, 2022

Language: Английский

Citations

154
All-Optical Electrophysiology Reveals the Role of Lateral Inhibition in Sensory Processing in Cortical Layer 1 DOI Creative Commons
Linlin Z. Fan, Simon Kheifets, Urs L. Böhm

et al.

Cell, Journal Year: 2020, Volume and Issue: 180(3), P. 521 - 535.e18

Published: Jan. 23, 2020

Language: Английский

Citations

147
A fluorescent sensor for spatiotemporally resolved imaging of endocannabinoid dynamics in vivo DOI
Ao Dong, Kaikai He, Barna Dudok

et al.

Nature Biotechnology, Journal Year: 2021, Volume and Issue: 40(5), P. 787 - 798

Published: Nov. 11, 2021

Language: Английский

Citations

131
Probing neural codes with two-photon holographic optogenetics DOI
Hillel Adesnik, Lamiae Abdeladim

Nature Neuroscience, Journal Year: 2021, Volume and Issue: 24(10), P. 1356 - 1366

Published: Aug. 16, 2021

Language: Английский

Citations

126
A unifying perspective on neural manifolds and circuits for cognition DOI
Christopher Langdon, Mikhail Genkin, Tatiana A. Engel

et al.

Nature reviews. Neuroscience, Journal Year: 2023, Volume and Issue: 24(6), P. 363 - 377

Published: April 13, 2023

Language: Английский

Citations

117
Fluorescence imaging of large-scale neural ensemble dynamics DOI Creative Commons
Tony Hyun Kim, Mark J. Schnitzer

Cell, Journal Year: 2022, Volume and Issue: 185(1), P. 9 - 41

Published: Jan. 1, 2022

Language: Английский

Citations

116
BiPOLES is an optogenetic tool developed for bidirectional dual-color control of neurons DOI Creative Commons
Johannes Vierock, Silvia Rodriguez-Rozada, Alexander Dieter

et al.

Nature Communications, Journal Year: 2021, Volume and Issue: 12(1)

Published: July 26, 2021

Abstract Optogenetic manipulation of neuronal activity through excitatory and inhibitory opsins has become an indispensable experimental strategy in neuroscience research. For many applications bidirectional control allowing both excitation inhibition the same neurons a single experiment is desired. This requires low spectral overlap between opsin, matched photocurrent amplitudes fixed expression ratio. Moreover, independent activation two distinct populations with different optogenetic actuators still challenging due to blue-light sensitivity all opsins. Here we report BiPOLES, tool for potent light wavelengths. BiPOLES enables sensitive, reliable dual-color spiking silencing single- or two-photon excitation, optical tuning membrane voltage, using second, sensitive opsin. The utility demonstrated worms, flies, mice ferrets.

Language: Английский

Citations

114
Structural basis for channel conduction in the pump-like channelrhodopsin ChRmine DOI Creative Commons
Koichiro Kishi, Yoon Seok Kim, Masahiro Fukuda

et al.

Cell, Journal Year: 2022, Volume and Issue: 185(4), P. 672 - 689.e23

Published: Feb. 1, 2022

ChRmine, a recently discovered pump-like cation-conducting channelrhodopsin, exhibits puzzling properties (large photocurrents, red-shifted spectrum, and extreme light sensitivity) that have created new opportunities in optogenetics. ChRmine its homologs function as ion channels but, by primary sequence, more closely resemble pump rhodopsins; mechanisms for passive channel conduction this family remained mysterious. Here, we present the 2.0 Å resolution cryo-EM structure of revealing architectural features atypical channelrhodopsins: trimeric assembly, short transmembrane-helix 3, twisting extracellular-loop 1, large vestibules within monomer, an opening at trimer interface. We applied to design three proteins (rsChRmine hsChRmine, conferring further high-speed properties, respectively, frChRmine, combining faster performance) suitable fundamental neuroscience opportunities. These results illuminate gating channelrhodopsins point way toward structure-guided creation applications across biology.

Language: Английский

Citations

109