Controlled Neighbor Exchanges Drive Glassy Behavior, Intermittency, and Cell Streaming in Epithelial Tissues DOI Creative Commons
Amit Das, Srikanth Sastry, Dapeng Bi

et al.

Physical Review X, Journal Year: 2021, Volume and Issue: 11(4)

Published: Nov. 22, 2021

Cell neighbor exchanges are integral to tissue rearrangements in biology, including development and repair. Often these processes occur via topological T1 transitions analogous those observed foams, grains colloids. However, contrast non-living materials the biological tissues rate-limited cannot instantaneously due finite time required remodel complex structures at cell-cell junctions. Here we study how this rate-limiting process affects mechanics collective behavior of cells a by introducing important constraint theoretical vertex-based model as an intrinsic single-cell property. We report absence constraint, undergoes motility-driven glass transition characterized sharp increase intermittency rearrangements. Remarkably, disappears temporally limited. As unique consequence limited rearrangements, also find that develops spatially correlated streams fast slow cells, which organize into stream-like patterns with leader-follower interactions, maintain optimally stable contacts. The predictions work is compared existing in-vivo experiments Drosophila pupal development.

Language: Английский

COVID-19 tissue atlases reveal SARS-CoV-2 pathology and cellular targets DOI Creative Commons
Toni Delorey, Carly G.K. Ziegler, Graham Heimberg

et al.

Nature, Journal Year: 2021, Volume and Issue: 595(7865), P. 107 - 113

Published: April 29, 2021

Language: Английский

Citations

721
Actin-based force generation and cell adhesion in tissue morphogenesis DOI Creative Commons
D. Nathaniel Clarke, Adam C. Martin

Current Biology, Journal Year: 2021, Volume and Issue: 31(10), P. R667 - R680

Published: May 1, 2021

Language: Английский

Citations

115
Dynamic changes in epithelial cell packing during tissue morphogenesis DOI Creative Commons
Sandra B. Lemke, Celeste M. Nelson

Current Biology, Journal Year: 2021, Volume and Issue: 31(18), P. R1098 - R1110

Published: Sept. 1, 2021

Language: Английский

Citations

64
Cytoskeletal prestress: The cellular hallmark in mechanobiology and mechanomedicine DOI Creative Commons
Farhan Chowdhury, Bo Huang, Ning Wang

et al.

Cytoskeleton, Journal Year: 2021, Volume and Issue: 78(6), P. 249 - 276

Published: March 23, 2021

Abstract Increasing evidence demonstrates that mechanical forces, in addition to soluble molecules, impact cell and tissue functions physiology diseases. How living cells integrate signals perform appropriate biological is an area of intense investigation. Here, we review the central role cytoskeletal prestress mechanotransduction mechanobiology. Elevating increases stiffness reinforces stiffening, facilitates long‐range cytoplasmic via integrins, enables direct chromatin stretching rapid gene expression, spurs embryonic development stem differentiation, boosts immune activation killing tumor whereas lowering maintains pluripotency, promotes tumorigenesis metastasis cell‐like malignant tumor‐repopulating cells, elevates drug delivery efficiency soft‐tumor‐cell‐derived microparticles. The overwhelming suggests governing principle cellular hallmark application mechanobiology medicine (mechanomedicine) rapidly emerging may help advance human health improve diagnostics, treatment, therapeutics

Language: Английский

Citations

42
Multiciliated cells use filopodia to probe tissue mechanics during epithelial integration in vivo DOI Creative Commons
Guilherme Ventura, Aboutaleb Amiri, Raghavan Thiagarajan

et al.

Nature Communications, Journal Year: 2022, Volume and Issue: 13(1)

Published: Oct. 28, 2022

Abstract During embryonic development, regeneration, and homeostasis, cells have to migrate physically integrate into the target tissues where they ultimately execute their function. While much is known about biochemical pathways driving cell migration in vivo, we are only beginning understand mechanical interplay between migrating surrounding tissue. Here, reveal that multiciliated precursors Xenopus embryo use filopodia pull at vertices of overlying epithelial sheet. This pulling effectively used sense vertex stiffness identify preferred positions for integration Notably, find forces equip with ability remodel junctions neighboring cells, enabling them generate a permissive environment facilitates integration. Our findings intricate physical crosstalk cell-tissue interface uncover previously unknown functions orchestrating

Language: Английский

Citations

30
Rap1 coordinates cell-cell adhesion and cytoskeletal reorganization to drive collective cell migration in vivo DOI Creative Commons
Katheryn E. Rothenberg, Yujun Chen, Jocelyn A. McDonald

et al.

Current Biology, Journal Year: 2023, Volume and Issue: 33(13), P. 2587 - 2601.e5

Published: May 26, 2023

Language: Английский

Citations

21
Mechanical force drives the initial mesenchymal-epithelial interaction during skin organoid development DOI Creative Commons
Mengyue Wang,

Xun Zhou,

Siyi Zhou

et al.

Theranostics, Journal Year: 2023, Volume and Issue: 13(9), P. 2930 - 2945

Published: Jan. 1, 2023

Rationale: Stem cells self-organize to form organoids that generate mini-organs resemble the physiologically-developed ones.The mechanism by which stem acquire initial potential for generating remains elusive.Here we used skin as an example study how mechanical force drives epidermal-dermal interaction potentiates regenerate hair follicles.Methods: Live imaging analysis, single-cell RNA-sequencing and immunofluorescence were analyze contractile of dermal in organoids.Bulk calcium probe detection, functional perturbations verify signaling pathways respond cells.In vitro loading experiment was prove stretching triggers epidermal Piezo1 expression negatively regulates cell attachment.Transplantation assay test regenerative ability organoids.Results: We found cell-derived contraction movement surrounding aggregates trigger mesenchymal-epithelial (MEI).In response force, arrangement cytoskeleton regulated pathway further influences dermal-epidermal attachment.The native generated from exerts a on adjacent cells, activating sensor basal during organoid culture.Epidermal turn strong MEI regulate attachment.Proper mechanical-chemical coupling culture is required regeneration upon transplantation into back nude mice. Conclusion:Our demonstrated cascade event development, fundamental organoid, developmental, biology fields.

Language: Английский

Citations

18
Mechanosensitive recruitment of Vinculin maintains junction integrity and barrier function at epithelial tricellular junctions DOI
Lotte van den Goor, Jolene Iseler,

Katherine Koning

et al.

Current Biology, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 1, 2024

Language: Английский

Citations

7
Assembly, dynamics and remodeling of epithelial cell junctions throughout development DOI
Marta Mira-Osuna, Roland Le Borgne

Development, Journal Year: 2024, Volume and Issue: 151(1)

Published: Jan. 1, 2024

ABSTRACT Cell junctions play key roles in epithelial integrity. During development, when epithelia undergo extensive morphogenesis, these must be remodeled order to maintain mechanochemical barriers and ensure the cohesion of tissue. In this Review, we present a comprehensive integrated description junctional remodeling mechanisms cells during from embryonic adult epithelia. We largely focus on Drosophila, as quantitative analyses organism have provided detailed characterization molecular governing cell topologies, discuss conservation across metazoans. consider how changes at level translate tissue-scale irreversible deformations, exploring composition assembly cellular interfaces unveil are preserve tissue homeostasis division, intercalation, invagination, ingression extrusion.

Language: Английский

Citations

5
Damage control of epithelial barrier function in dynamic environments DOI Creative Commons
Tomohito Higashi, Akira Saitō, Hideki Chiba

et al.

European Journal of Cell Biology, Journal Year: 2024, Volume and Issue: 103(2), P. 151410 - 151410

Published: April 3, 2024

Epithelial tissues cover the surfaces and lumens of internal organs multicellular animals crucially contribute to environment homeostasis by delineating distinct compartments within body. This vital role is known as epithelial barrier function. cells are arranged like cobblestones intricately bind together form an sheet that upholds this Central restriction solute fluid diffusion through intercellular spaces occluding junctions, tight junctions in vertebrates septate invertebrates. As part tissues, undergo constant renewal, with older being replaced new ones. Simultaneously, tissue undergoes relative rearrangement, elongating, shifting directionally a whole. The movement or shape changes necessitate significant deformation reconnection junctions. Recent advancements have shed light on intricate mechanisms which sustain their function dynamic environments. review aims introduce these noteworthy findings discuss some questions remain unanswered.

Language: Английский

Citations

5