Voltage dynamics of dendritic integration and back-propagationin vivo DOI Creative Commons

J. David Wong-Campos,

Pojeong Park,

Hunter C. Davis

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: May 26, 2023

Neurons integrate synaptic inputs within their dendrites and produce spiking outputs, which then propagate down the axon back into where they contribute to plasticity. Mapping voltage dynamics in dendritic arbors of live animals is crucial for understanding neuronal computation plasticity rules. Here we combine patterned channelrhodopsin activation with dual-plane structured illumination imaging, simultaneous perturbation monitoring somatic Layer 2/3 pyramidal neurons anesthetized awake mice. We examined integration compared optogenetically evoked, spontaneous, sensory-evoked back-propagating action potentials (bAPs). Our measurements revealed a broadly shared membrane throughout arbor, few signatures electrical compartmentalization among inputs. However, observed spike rate acceleration-dependent propagation bAPs distal dendrites. propose that this filtering may play critical role activity-dependent

Language: Английский

A local circuit-basis for spatial navigation and memory processes in hippocampal area CA1 DOI Creative Commons
Tristan Geiller, James B. Priestley, Attila Losonczy

et al.

Current Opinion in Neurobiology, Journal Year: 2023, Volume and Issue: 79, P. 102701 - 102701

Published: March 4, 2023

Language: Английский

Citations

19
The dendritic engram DOI Creative Commons
George Kastellakis,

Simone Tasciotti,

Ioanna Pandi

et al.

Frontiers in Behavioral Neuroscience, Journal Year: 2023, Volume and Issue: 17

Published: July 27, 2023

Accumulating evidence from a wide range of studies, including behavioral, cellular, molecular and computational findings, support key role dendrites in the encoding recall new memories. Dendrites can integrate synaptic inputs non-linear ways, provide substrate for local protein synthesis facilitate orchestration signaling pathways that regulate plasticity. These capabilities allow them to act as second layer computation within neuron serve fundamental unit As such, are integral parts memory engram, namely physical representation memories brain increasingly studied during learning tasks. Here, we review experimental studies novel, dendritic view engram is centered on branches elementary units. We highlight potential implications engrams field discuss future research directions.

Language: Английский

Citations

18
Synaptic Basis of Behavioral Timescale Plasticity DOI Open Access
Kevin C. Gonzalez, Adrian Negrean, Zhenrui Liao

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 5, 2023

Abstract Learning and memory are fundamental to adaptive behavior cognition. Various forms of synaptic plasticity have been proposed as cellular substrates for the emergence feature selectivity in neurons underlying episodic memory. However, despite decades work, our understanding how underlies encoding remains limited, largely due a shortage tools technical challenges associated with visualization at single-neuron resolution awake-behaving animals. Behavioral Timescale Synaptic Plasticity (BTSP) postulates that inputs active during seconds-long time window preceding immediately following large depolarizing plateau spike potentiated, while outside this depressed. We experimentally tested model vivo mice using an all-optical approach by inducing place fields (PFs) single CA1 pyramidal (CA1PNs) monitoring spatiotemporal tuning individual dendritic spines changes their corresponding weights. identified asymmetric kernel resulting from bidirectional modifications weights around burst induction. Surprisingly, work also uncovered compartment-specific differences magnitude temporal expression between basal oblique dendrites CA1PNs. Our results provide first experimental evidence linking rapid spatial hippocampal neurons, critical prerequisite

Language: Английский

Citations

17
Periodic ER-plasma membrane junctions support long-range Ca2+ signal integration in dendrites DOI Creative Commons
Lorena Benedetti, Ruolin Fan, Aubrey V. Weigel

et al.

Cell, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 1, 2024

Language: Английский

Citations

7
A biochemical description of postsynaptic plasticity—with timescales ranging from milliseconds to seconds DOI Creative Commons

Guanchun Li,

David W. McLaughlin,

Charles S. Peskin

et al.

Proceedings of the National Academy of Sciences, Journal Year: 2024, Volume and Issue: 121(7)

Published: Feb. 7, 2024

Synaptic plasticity [long-term potentiation/depression (LTP/D)], is a cellular mechanism underlying learning. Two distinct types of early LTP/D (E-LTP/D), acting on very different time scales, have been observed experimentally—spike timing dependent (STDP), scales tens ms; and behavioral scale synaptic (BTSP), seconds. BTSP candidate for rapid learning spatial location by place cells. Here, computational model the induction E-LTP/D at spine head synapse hippocampal pyramidal neuron developed. The single-compartment represents two interacting biochemical pathways activation (phosphorylation) kinase (CaMKII) with phosphatase, ion inflow through channels (NMDAR, CaV1,Na). reactions are represented deterministic system differential equations, detailed description CaMKII that includes opening compact state CaMKII. This single captures realistic responses (temporal profiles differing timescales) STDP their asymmetries. simulations distinguish several mechanisms vs. BTSP, including i) flow Ca 2 + NMDAR CaV1 channels, ii) origin in also realizes priming E-LTP induced CaV1.3 channels. Once head, this small additional opens CaMKII, placing ready subsequent LTP.

Language: Английский

Citations

6
Calcium and activity-dependent signaling in the developing cerebral cortex DOI Creative Commons

Arpana Arjun McKinney,

Ralitsa Petrova, Georgia Panagiotakos

et al.

Development, Journal Year: 2022, Volume and Issue: 149(17)

Published: Sept. 1, 2022

Calcium influx can be stimulated by various intra- and extracellular signals to set coordinated gene expression programs into motion. As such, the precise regulation of intracellular calcium represents a nexus between environmental cues intrinsic genetic programs. Mounting evidence points role for deregulation signaling in neuropsychiatric disorders developmental origin. These findings have prompted renewed enthusiasm understanding roles during normal dysfunctional prenatal development. In this Review, we describe fundamental mechanisms through which is spatiotemporally regulated directs early neurodevelopmental events. We also discuss unanswered questions about emergence disease, provide that disruption cell-specific homeostasis and/or redeployment may contribute adult neurological disorders. propose events build nervous system will rely on gaining insights cell type-specific mechanisms. Such an enable therapeutic strategies targeting calcium-dependent mitigate disease.

Language: Английский

Citations

26
Latent representations in hippocampal network model co-evolve with behavioral exploration of task structure DOI Creative Commons
Ian Cone, Claudia Clopath

Nature Communications, Journal Year: 2024, Volume and Issue: 15(1)

Published: Jan. 23, 2024

To successfully learn real-life behavioral tasks, animals must pair actions or decisions to the task's complex structure, which can depend on abstract combinations of sensory stimuli and internal logic. The hippocampus is known develop representations this forming a so-called "cognitive map". However, precise biophysical mechanisms driving emergence task-relevant maps at population level remain unclear. We propose model in plateau-based learning single cell level, combined with reinforcement an agent, leads latent representational structures codependently evolving behavior task-specific manner. In agreement recent experimental data, we show that develops essential for task-solving (cue-dependent "splitters") while excluding irrelevant ones. Finally, our makes testable predictions concerning co-dependent interactions between split policy during their evolution.

Language: Английский

Citations

5
Activity-dependent mitochondrial ROS signaling regulates recruitment of glutamate receptors to synapses DOI Creative Commons
Rachel L. Doser, Kaz M. Knight, Ennis W. Deihl

et al.

eLife, Journal Year: 2024, Volume and Issue: 13

Published: March 14, 2024

Our understanding of mitochondrial signaling in the nervous system has been limited by technical challenge analyzing function vivo. In transparent genetic model Caenorhabditis elegans, we were able to manipulate and measure reactive oxygen species (mitoROS) individual mitochondria as well neuronal activity single neurons Using this approach, provide evidence supporting a novel role for mitoROS dendrites excitatory glutamatergic C. elegans interneurons. Specifically, show that following activity, dendritic take up calcium (Ca2+) via Ca2+ uniporter (MCU-1) results an upregulation production. We also observed are positioned close proximity synaptic clusters GLR-1, ortholog AMPA subtype glutamate receptors mediate excitation. recruitment GLR-1 is upregulated when MCU-1 pharmacologically or genetically impaired but downregulated signaling. Thus, from postsynaptic may regulate synapse maintain homeostasis preventing excitotoxicity energy depletion.

Language: Английский

Citations

5
Synaptic basis of feature selectivity in hippocampal neurons DOI
Kevin C. Gonzalez, Adrian Negrean, Zhenrui Liao

et al.

Nature, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 18, 2024

Language: Английский

Citations

5
Dendritic, delayed, and stochastic CaMKII activation underlies behavioral time scale plasticity in CA1 synapses DOI Open Access
Anant Jain, Yoshihisa Nakahata, Tetsuya Watabe

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2023, Volume and Issue: unknown

Published: Aug. 1, 2023

Behavioral time scale plasticity (BTSP), is a form of non-Hebbian induced by integrating pre- and postsynaptic components separated behavioral (seconds). BTSP in the hippocampal CA1 neurons underlies place cell formation. However, molecular mechanisms underlying this (eligibility trace) synapse specificity are unknown. CaMKII can be activated synapse-specific manner remain active for few seconds, making it compelling candidate eligibility trace during BTSP. Here, we show that single dendritic spine using 2-photon glutamate uncaging paired with current injection temporally scale. Using an improved sensor, saw no detectable activation induction. Instead, observed dendritic, delayed, stochastic (DDSC) associated Ca

Language: Английский

Citations

12