Frontiers in Neuroscience,
Journal Year:
2022,
Volume and Issue:
16
Published: Nov. 16, 2022
Radial
glia
is
a
cell
type
traditionally
associated
with
the
developing
nervous
system,
particularly
formation
of
cortical
layers
in
mammalian
brain.
Nonetheless,
some
these
cells,
or
closely
related
types,
called
radial
glia-like
cells
are
found
adult
central
system
structures,
functioning
as
neurogenic
progenitors
normal
homeostatic
maintenance
and
response
to
injury.
The
heterogeneity
nowadays
being
probed
molecular
tools,
primarily
by
expression
specific
genes
that
define
types.
Similar
markers
have
identified
non-vertebrate
organisms.
In
this
review,
we
focus
on
processes
during
homeostasis
We
highlight
our
results
using
model
echinoderm
Advanced Science,
Journal Year:
2023,
Volume and Issue:
10(16)
Published: April 7, 2023
Spatial
transcriptomics
is
a
newly
emerging
field
that
enables
high-throughput
investigation
of
the
spatial
localization
transcripts
and
related
analyses
in
various
applications
for
biological
systems.
By
transitioning
from
conventional
studies
to
"in
situ"
biology,
can
provide
transcriptome-scale
information.
Currently,
ability
simultaneously
characterize
gene
expression
profiles
cells
relevant
cellular
environment
paradigm
shift
studies.
In
this
review,
recent
progress
its
neuroscience
cancer
are
highlighted.
Technical
aspects
existing
technologies
future
directions
new
developments
(as
March
2023),
computational
analysis
transcriptome
data,
application
notes
studies,
discussions
regarding
multi-omics
their
expanding
roles
biomedical
emphasized.
Cells,
Journal Year:
2023,
Volume and Issue:
12(16), P. 2042 - 2042
Published: Aug. 10, 2023
Spatial
transcriptomic
technologies
enable
measurement
of
expression
levels
genes
systematically
throughout
tissue
space,
deepening
our
understanding
cellular
organizations
and
interactions
within
tissues
as
well
illuminating
biological
insights
in
neuroscience,
developmental
biology
a
range
diseases,
including
cancer.
A
variety
spatial
have
been
developed
and/or
commercialized,
differing
resolution,
sensitivity,
multiplexing
capability,
throughput
coverage.
In
this
paper,
we
review
key
enabling
their
applications
the
perspective
techniques
new
emerging
that
are
to
address
current
limitations
methodologies.
addition,
describe
how
transcriptomics
data
can
be
integrated
with
other
omics
modalities,
complementing
methods
deciphering
cellar
phenotypes
providing
novel
insight
into
organization.
The Plant Journal,
Journal Year:
2023,
Volume and Issue:
116(6), P. 1571 - 1581
Published: Aug. 31, 2023
SUMMARY
The
plant
community
lags
far
behind
the
animal
and
human
fields
concerning
application
of
single‐cell
methodologies.
This
is
primarily
due
to
challenges
associated
with
tissue
dissection
limitations
available
technologies.
However,
recent
advances
in
spatial
transcriptomics
enable
study
single‐cells
derived
from
tissues
a
perspective.
technology
already
successfully
used
identify
cell
types,
reconstruct
cell‐fate
lineages,
reveal
cell‐to‐cell
interactions.
Future
technological
advancements
will
overcome
sample
processing,
data
analysis,
integration
multiple‐omics
Thanks
transcriptomics,
we
anticipate
several
research
projects
significantly
advance
our
understanding
critical
aspects
biology.
Nature Ecology & Evolution,
Journal Year:
2023,
Volume and Issue:
7(10), P. 1714 - 1728
Published: Sept. 14, 2023
Abstract
The
vertebrate
brain
emerged
more
than
~500
million
years
ago
in
common
evolutionary
ancestors.
To
systematically
trace
its
cellular
and
molecular
origins,
we
established
a
spatially
resolved
cell
type
atlas
of
the
entire
sea
lamprey—a
jawless
species
whose
phylogenetic
position
affords
reconstruction
ancestral
traits—based
on
extensive
single-cell
RNA-seq
situ
sequencing
data.
Comparisons
this
to
neural
data
from
mouse
other
jawed
vertebrates
unveiled
various
shared
features
that
enabled
types,
tissue
structures
gene
expression
programs
brain.
However,
our
analyses
also
revealed
key
tissues
types
arose
later
evolution.
For
example,
was
probably
devoid
cerebellar
oligodendrocytes
(myelinating
cells);
suggest
latter
astrocyte-like
precursors
lineage.
Altogether,
work
illuminates
architecture
provides
foundation
for
exploring
diversification
during
Experimental & Molecular Medicine,
Journal Year:
2023,
Volume and Issue:
55(10), P. 2105 - 2115
Published: Oct. 2, 2023
Abstract
The
brain
is
one
of
the
most
complex
living
tissue
types
and
composed
an
exceptional
diversity
cell
displaying
unique
functional
connectivity.
Single-cell
RNA
sequencing
(scRNA-seq)
can
be
used
to
efficiently
map
molecular
identities
various
in
by
providing
transcriptomic
profiles
individual
cells
isolated
from
tissue.
However,
lack
spatial
context
scRNA-seq
prevents
a
comprehensive
understanding
how
different
configurations
give
rise
specific
functions
regions
each
distinct
connected
form
unit.
To
understand
contribute
functions,
it
crucial
correlate
obtained
through
with
their
information
intact
Spatial
transcriptomics
(ST)
resolve
organization
Various
ST
tools
developed
during
past
decade
based
on
imaging
technology
have
permitted
creation
atlases
pulled
properties
neural
circuits
into
ever-sharper
focus.
In
this
review,
we
present
summary
several
applications
neuroscience
discuss
unprecedented
insights
these
made
possible.
