bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 5, 2023
Abstract
3D
organization
of
the
genome
plays
a
critical
role
in
regulating
gene
expression.
However,
it
remains
unclear
how
chromatin
differs
among
different
cell
types
brain.
Here
we
used
genome-scale
DNA
and
RNA
imaging
to
investigate
3D-genome
transcriptionally
distinct
primary
motor
cortex
mouse
We
uncovered
wide
spectrum
differences
nuclear
architecture
types,
ranging
from
physical
size
nucleus
active-inactive
compartmentalization
radial
positioning
loci
within
nucleus.
These
cell-type-dependent
variations
exhibited
strong
correlation
with
both
total
transcriptional
activity
regulation
cell-type-specific
marker
genes.
Moreover,
found
that
methylated-DNA-binding
protein
MeCP2
regulates
transcription
divergent
manner,
depending
on
positions
loci,
through
modulating
compartmentalization.
Biology Open,
Journal Year:
2025,
Volume and Issue:
14(5)
Published: May 12, 2025
ABSTRACT
The
3D
organization
of
the
genome
has
emerged
as
an
important
regulator
cellular
development.
Post-mitotic
neurons
undergo
conserved
changes
in
organization,
such
inward
radial
repositioning
heterochromatin-rich
chromosomes
they
differentiate.
Additionally,
transcriptionally
active
but
heterochromatin-associated
gene-dense
(hGD)
regions
significantly
strengthen
their
long-distance
interactions
during
cerebellar
However,
specific
developmental
stages
which
these
nuclear
take
place
have
remained
poorly
defined.
Here,
we
report
that
hGD
relocalize
toward
interior
and
chromosomal
immature
granule
transition
from
cell
migration
to
subsequent
neuronal
differentiation.
During
this
period,
genomic
are
coordinately
repositioned
nucleus
alongside
physically
tethered
heterochromatic
chromocenters.
Despite
major
subcompartment
remains
distinct
other
or
repressive
bodies,
including
chromocenters,
throughout
Notably,
appear
be
independent
transcriptional
occur
neuron
Together,
our
results
provide
insights
into
timing
structural
post-mitotic
neurons.
The EMBO Journal,
Journal Year:
2024,
Volume and Issue:
43(16), P. 3312 - 3326
Published: July 15, 2024
Abstract
Aging
is
associated
with
a
progressive
decline
of
brain
function,
and
the
underlying
causes
possible
interventions
to
prevent
this
cognitive
have
been
focus
intense
investigation.
The
maintenance
neuronal
function
over
lifespan
requires
proper
epigenetic
regulation,
accumulating
evidence
suggests
that
deterioration
landscape
contributes
dysfunction
during
aging.
Epigenetic
aging
neurons
may,
however,
be
malleable.
Recent
reports
shown
age-related
changes
in
reversible
targetable
by
rejuvenation
strategies
can
restore
This
review
discusses
current
identifies
as
driver
promising
target
strategies,
it
highlights
potential
approaches
for
specific
manipulation
epigenome
youthful
state
brain.
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 5, 2023
Abstract
3D
organization
of
the
genome
plays
a
critical
role
in
regulating
gene
expression.
However,
it
remains
unclear
how
chromatin
differs
among
different
cell
types
brain.
Here
we
used
genome-scale
DNA
and
RNA
imaging
to
investigate
3D-genome
transcriptionally
distinct
primary
motor
cortex
mouse
We
uncovered
wide
spectrum
differences
nuclear
architecture
types,
ranging
from
physical
size
nucleus
active-inactive
compartmentalization
radial
positioning
loci
within
nucleus.
These
cell-type-dependent
variations
exhibited
strong
correlation
with
both
total
transcriptional
activity
regulation
cell-type-specific
marker
genes.
Moreover,
found
that
methylated-DNA-binding
protein
MeCP2
regulates
transcription
divergent
manner,
depending
on
positions
loci,
through
modulating
compartmentalization.
