Oncogene, Journal Year: 2023, Volume and Issue: 42(10), P. 737 - 747
Published: Jan. 5, 2023
Language: Английский
Electrochimica Acta, Journal Year: 2024, Volume and Issue: 487, P. 144205 - 144205
Published: April 1, 2024
Triple-negative breast cancer (TNBC) is one of the most aggressive and lethal types BC, affecting mostly young women its diagnosis difficult requires invasive methods, such as tissue biopsy which painful expensive. However, nowadays liquid emerging a great tool for determining blood-circulating species associated to early prognosis. Among species, relevance microRNAs (miRNAs) has been highlighted promising biomarker, miRNA-652-5p with TNBC it promotes growth migration cells. In this work we designed characterized paper-based electrochemical device capable recognizing quantifying miRNA-652-5p, future in TNBC. The consists an AuNP-modified office screen-printed electrode customized anti-miRNA probe selective recognition miRNA-652-5p. All experimental parameters have carefully evaluated, platform allowed detect standard solution human serum down 0.4 nM, satisfactory repeatability about 6 3% respectively. selectivity presence other miRNA sequences was demonstrated. addition, demonstrated effectiveness pre-concentration by coupling external disk made chromatographic paper: detection limit improved 10-fold without use complex/expensive procedures. presented manuscript represents important step towards development non-invasive, sensitive TNBC-specific diagnostic that could improve patients' prognosis quality life, ulteriorly pre-concentering properties frugal supports ones.
Language: Английский
Citations
10
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Jan. 10, 2025
Biological processes intricately intertwine with tumorigenesis, significantly influencing treatment outcomes and prognosis. However, the mechanisms fostering mucoepidermoid carcinoma (MEC) remain inadequately elucidated. This research utilizes expression profiles of lncRNAs from clinical MEC tissues matched normal glandular tissues, integrating public data to explore biological immune microenvironment characteristics tumorigenesis. Gene set enrichment analysis identified key pathways, a customized epithelial-mesenchymal transition (EMT) score elucidated relationship between pathological prognosis, while an signature revealed tumor characteristics. MECs exhibited significant in EMT pathway, genes such as Secretogranin II, tissue factor pathway inhibitor 2, periostin contributors process. High scores correlated upregulated response activity, indicating poor Single-sample gene unveiled tumors' infiltration signature, suggesting active antigen presentation positive for immunotherapy. Additionally, SLC2A1-AS1 CERS6-AS1 were potential mediators environment. study provides insights into tumorigenesis identifies therapeutic targets future research.
Language: Английский
Citations
1
Seminars in Cancer Biology, Journal Year: 2023, Volume and Issue: 95, P. 120 - 139
Published: Aug. 10, 2023
Cancer cells adapt to varying stress conditions survive through plasticity. Stem exhibit a high degree of plasticity, allowing them generate more stem or differentiate into specialized cell types contribute tissue development, growth, and repair. can also plasticity acquire properties that enhance their survival. TGF-β is an unrivaled growth factor exploited by cancer gain TGF-β-mediated signaling enables carcinoma alter epithelial mesenchymal epithelial-mesenchymal (EMP). However, multifunctional cytokine; thus, the be detrimental beneficial depending on cellular context. Those overcome anti-tumor effect induce transition (EMT) EMP benefits. allows tumor immune microenvironment (TIME), facilitating Due significant roles in progression, it essential understand how exploit this This understanding will guide development effective TGF-β-targeting therapies eliminate
Language: Английский
Citations
23
Aggregate, Journal Year: 2024, Volume and Issue: unknown
Published: June 25, 2024
Abstract The poor prognosis of triple‐negative breast cancer (TNBC) results from its high metastasis, whereas inflammation accompanied by excessive reactive oxygen species (ROS) is prone to aggravate tumor metastasis. Although photothermal therapy (PTT) has extremely therapeutic efficiency, the crafty cells allow an increase in expression heat shock proteins (HSPs) limit effect, and PTT‐induced also thought be a potential trigger for Herein, myricetin, iron ions, polyvinylpyrrolidone were utilized develop nanomedicines self‐assembly strategy treatment metastatic TNBC. with marvelous water solubility dispersion can inhibit glucose transporter 1 interfere mitochondrial function block energy supply cells, achieving starvation on TNBC cells. Nanomedicines excellent conversion properties down‐regulating HSPs enhance effect PTT. Interestingly, broad spectrum ROS scavenging ability successfully attenuates as well influences hypoxia‐inducible factors‐1α/3‐phosphoinositide‐dependent protein kinase related pathway through glycometabolism inhibition reduce cell Moreover, have negligible side effects good clinical application prospects, which provides valuable paradigm interference, anti‐inflammation, starvation, synergistic therapy.
Language: Английский
Citations
6
Disease Models & Mechanisms, Journal Year: 2024, Volume and Issue: 17(3)
Published: March 1, 2024
ABSTRACT Breast cancer stands as the most prevalent malignancy afflicting women. Despite significant advancements in its diagnosis and treatment, breast metastasis continues to be a leading cause of mortality among To metastasize, cells face numerous challenges: breaking away from primary tumor, surviving circulation, establishing distant location, evading immune detection and, finally, thriving initiate new tumor. Each these sequential steps requires adapt myriad stressors develop survival mechanisms. In addition, patients with undergo surgical removal their tumor have various therapeutic interventions designed eradicate cells. this plethora attacks stresses, certain not only manage persist but also proliferate robustly, giving rise substantial tumors that frequently culminate patient's demise. enhance patient outcomes, there is an imperative need for deeper understanding molecular cellular mechanisms empower survive expand. Herein, we delve into intrinsic stresses encounter throughout metastatic journey additional induced by interventions. We focus on elucidating remarkable strategies adopted cells, such cell–cell clustering intricate communication mechanisms, ensure survival.
Language: Английский
Citations
5
iScience, Journal Year: 2024, Volume and Issue: 27(7), P. 110116 - 110116
Published: May 27, 2024
Highlights•Luminal signature is closely associated with epithelial in breast cancer•Basal correlates well a hybrid epithelial-mesenchymal signature•Basal cancer exhibits higher heterogeneity patterns•Mathematical modeling of underlying gene networks explains observed heterogeneitySummaryIntra-tumoral phenotypic promotes tumor relapse and therapeutic resistance remains an unsolved clinical challenge. Decoding the interconnections among different biological axes plasticity crucial to understand molecular origins heterogeneity. Here, we use multi-modal transcriptomic data—bulk, single-cell, spatial transcriptomics—from cell lines primary samples, identify associations between transition (EMT) luminal-basal plasticity—two key processes that enable We show luminal strongly associates state, but basal epithelial/mesenchymal phenotype(s) Mathematical core regulatory representative crosstalk elucidate mechanistic underpinnings from data. Our systems-based approach integrating data analysis mechanism-based offers predictive framework characterize intra-tumor interventions restrict it.Graphical abstract
Language: Английский
Citations
5
Drug Resistance Updates, Journal Year: 2024, Volume and Issue: 76, P. 101119 - 101119
Published: July 14, 2024
Language: Английский
Citations
5
Advanced Science, Journal Year: 2024, Volume and Issue: unknown
Published: July 29, 2024
Aggressive triple-negative breast cancer (TNBC) still lacks approved targeted therapies, requiring more exploration of its underlying mechanisms. Previous studies have suggested a potential role SAT1 (Spermidine/Spermine N1-acetyltransferase 1) in cancer, which needs to be further elucidated cancer. In this study, highly expressed TNBC signified worse patient prognoses. And knockdown effectively inhibited the proliferation and migration abilities cells vitro vivo. terms mechanism, transcription factor JUN enhanced transcriptional activity by binding promoter region. Then, protein cytoplasm engaged directly with YBX1 for sustaining stability via deubiquitylation mediated E3 ligase HERC5. Further, was found suppress autophagy remarkably stabilization mTOR mRNA accumulation YBX1-mediated methyl-5-cytosine (m5C) modification. These findings proved that drives progression through SAT1/YBX1/mTOR axis, may provide candidate therapy advanced TNBC.
Language: Английский
Citations
5
Nature Medicine, Journal Year: 2024, Volume and Issue: 30(11), P. 3236 - 3249
Published: Oct. 30, 2024
Abstract Although metastatic disease is the leading cause of cancer-related deaths, its tumor microenvironment remains poorly characterized due to technical and biospecimen limitations. In this study, we assembled a multi-modal spatial cellular map 67 biopsies from 60 patients with breast cancer across diverse clinicopathological features nine anatomic sites detailed clinical annotations. We combined single-cell or single-nucleus RNA sequencing for all panel four expression assays (Slide-seq, MERFISH, ExSeq CODEX) H&E staining consecutive serial sections up 15 these biopsies. leveraged coupled measurements provide reference points utility integration different experimental techniques used them assess variability in cell type composition as well emerging characteristics methodological diversity. Finally, assessed co-localization macrophage populations, three distinct phenotypes epithelial-to-mesenchymal transition identified programs associated local T infiltration versus exclusion, showcasing potential clinically relevant discovery such maps.
Language: Английский
Citations
5
Journal of Translational Medicine, Journal Year: 2023, Volume and Issue: 21(1)
Published: Oct. 13, 2023
Abstract Background Polyploid giant cancer cells (PGCCs), a specific type of stem (CSCs), can be induced by hypoxic microenvironments, chemical reagents, radiotherapy, and Chinese herbal medicine. Moreover, PGCCs produce daughter that undergo epithelial–mesenchymal transition, which leads to recurrence disseminated metastasis. Vimentin, mesenchymal cell marker, is highly expressed in their (PDCs) drives migratory persistence. This study explored the molecular mechanisms vimentin synergistically regulates generate with enhanced invasive metastatic properties. Methods Arsenic trioxide (ATO) was used induce formation Hct116 LoVo cells. Immunocytochemical immunohistochemical assays were performed determine subcellular localization vimentin. Cell function compare abilities PDCs control The underlying expression nuclear translocation investigated real-time polymerase chain reaction, western blotting, assays, transfection, co-immunoprecipitation, chromatin immunoprecipitation, followed sequencing. Finally, animal xenograft experiments clinical colorectal samples tumor tissues. Results Daughter derived from showed strong proliferative, migratory, abilities, located both cytoplasm nucleus. Vimentin undergoes small ubiquitin-like modification (SUMOylation) interacting SUMO1 SUMO2/3, are associated translocation. P62 controlling SUMO2/3 expression. In nucleus, acts as transcription factor CDC42 , cathepsin B, D promote PDC invasion migration. Furthermore, human specimens have confirmed Conclusion P62-dependent SUMOylation plays an important role migration invasion. overexpressed may predict patient prognosis, targeting promising therapeutic strategy for cancers.
Language: Английский
Citations
12