bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2024,
Volume and Issue:
unknown
Published: March 14, 2024
Summary
Liver
sinusoidal
endothelial
cells
(LSECs)
critically
regulate
homeostatic
liver
function
and
pathogenesis.
However,
the
isolation
of
LSECs
remains
a
major
technological
bottleneck
in
studying
molecular
mechanisms
governing
LSEC
functions.
Current
techniques
to
isolate
LSECs,
relying
on
perfusion-dependent
digestion,
are
cumbersome
with
limited
throughput.
We
here
describe
perfusion-independent
high-throughput
procedure
high
purity.
Indifferently
from
previous
approaches,
chopped
tissue
was
incubated
digestion
mix
for
30
minutes
intermittent
mixing
serological
pipette.
This
led
safeguarding
integrity
yielded
10
±
1.0
million
per
adult
mouse
liver,
which
is
far
higher
than
protocols
comparable
yield
established
isolating
LSECs.
Combining
magnetic
fluorescence-activated
cell
sorting
(FACS),
different
zones
hepatic
sinusoid
can
now
be
isolated
numbers
less
two
hours
downstream
applications
including
proteomics.
Our
protocol
enabled
fibrotic
tissues
mice
healthy
vertebrate
species
(pigs),
where
traditional
perfusion-based
have
very
application.
In
conclusion,
these
technical
advancements
reduce
post-mortem
changes
state
aid
reliable
investigation
Frontiers in Cell and Developmental Biology,
Journal Year:
2025,
Volume and Issue:
12
Published: Feb. 12, 2025
Chronic
kidney
disease
(CKD)
is
a
progressive
condition
that
can
lead
to
chronic
renal
failure
(CRF),
affecting
8%–16%
of
adults
globally
and
imposing
significant
burden
on
healthcare
systems.
Renal
fibrosis
key
pathological
hallmark
CKD
progression
linked
poor
prognosis.
Multiple
signaling
pathways,
including
WNT/β-catenin.Aberrant
activation
WNT/β-catenin
implicated
in
fibrosis.
The
roles
macrophages
fibroblasts
are
pivotal
Communications Biology,
Journal Year:
2025,
Volume and Issue:
8(1)
Published: Jan. 8, 2025
Abstract
Liver
sinusoidal
endothelial
cells
(LSECs)
critically
regulate
homeostatic
liver
function
and
pathogenesis.
However,
the
isolation
of
LSECs
remains
a
major
technological
bottleneck
in
studying
molecular
mechanisms
governing
LSEC
functions.
Current
techniques
to
isolate
LSECs,
relying
on
perfusion-dependent
digestion,
are
cumbersome
with
limited
throughput.
We
here
describe
perfusion-independent
high-throughput
procedure
high
purity.
Indifferently
from
previous
approaches,
chopped
tissue
was
incubated
digestion
mix
for
30
minutes
intermittent
mixing
serological
pipette.
This
led
safeguarding
integrity
yielded
10
±
1.0
million
per
adult
mouse
liver,
which
is
far
higher
than
protocols
comparable
yield
established
isolating
LSECs.
Combining
magnetic
fluorescence-activated
cell
sorting
(FACS),
different
zones
hepatic
sinusoid
can
now
be
isolated
numbers
less
two
hours
downstream
applications
including
proteomics.
Our
protocol
enables
fibrotic
tissues
mice
healthy
vertebrate
species
(pigs),
where
traditional
perfusion-based
have
very
application.
In
conclusion,
these
technical
advancements
reduce
post-mortem
changes
state
aid
reliable
investigation
Cells,
Journal Year:
2025,
Volume and Issue:
14(2), P. 105 - 105
Published: Jan. 13, 2025
Tissue
fibrosis
results
from
a
dysregulated
and
chronic
wound
healing
response
accompanied
by
inflammation
angiogenesis.
Regardless
of
the
affected
organ,
shares
following
common
hallmarks:
recruitment
immune
cells,
fibroblast
activation/proliferation,
excessive
extracellular
matrix
deposition.
Chemokines
play
pivotal
role
in
initiating
advancing
these
fibrotic
processes.
CCL24
(eotaxin-2)
is
chemokine
secreted
cells
epithelial
which
promotes
trafficking
activation
profibrotic
through
CCR3
receptor
binding.
Higher
levels
were
found
tissue
sera
patients
with
fibro-inflammatory
diseases,
including
primary
sclerosing
cholangitis
(PSC),
systemic
sclerosis
(SSc),
metabolic
dysfunction-associated
steatohepatitis
(MASH).
This
review
delves
into
intricate
highlighting
its
impact
on
fibrotic,
immune,
vascular
pathways.
We
focus
preclinical
clinical
evidence
supporting
therapeutic
potential
blocking
diseases
that
involve
fibrosis.
Frontiers in Pharmacology,
Journal Year:
2025,
Volume and Issue:
16
Published: Feb. 5, 2025
The
SOX9
gene
locus
is
not
only
extensive
but
also
intricate,
and
it
could
promote
fibrosis
in
different
organs
or
tissues,
including
cardiac
fibrosis,
liver
kidney
pulmonary
as
well
other
organ
fibrosis.
Many
disorders
are
associated
with
the
process
of
fibrosis;
moreover,
a
common
symptom
chronic
inflammatory
diseases,
characterized
by
accumulation
excessive
components
extracellular
matrix
through
signaling
pathways.
advanced
stage
fibrotic
leads
to
dysfunction
and,
ultimately,
death.
In
this
review,
we
first
give
an
overview
original
structure
functions
SOX9.
Second,
will
discuss
role
various
tissues.
Third,
describe
reveal
possibility
antifibrotic
treatment
target.
Finally,
focus
on
application
novel
technologies
for
subsequent
investigation
Science Advances,
Journal Year:
2024,
Volume and Issue:
10(32)
Published: Aug. 9, 2024
Endothelial
cells
(ECs)
are
highly
plastic,
capable
of
differentiating
into
various
cell
types.
Endothelial-to-mesenchymal
transition
(EndMT)
is
crucial
during
embryonic
development
and
contributes
substantially
to
vascular
dysfunction
in
many
cardiovascular
diseases
(CVDs).
While
targeting
EndMT
holds
therapeutic
promise,
understanding
its
mechanisms
modulating
pathways
remain
challenging.
Using
single-cell
RNA
sequencing
on
three
vitro
models,
we
identified
conserved
gene
signatures.
We
validated
original
regulators
vivo
heart
peripheral
artery
disease.
induction
led
global
expression
changes
all
EC
subtypes
rather
than
mesenchymal
clusters.
mitochondrial
calcium
uptake
as
a
key
driver
EndMT;
inhibiting
uniporter
(MCU)
prevented
vitro,
conditional
