Syntrophy Goes Electric: Direct Interspecies Electron Transfer

Annual Review of Microbiology, Journal Year: 2017, Volume and Issue: 71(1), P. 643 - 664

Published: July 12, 2017

Direct interspecies electron transfer (DIET) has biogeochemical significance, and practical applications that rely on DIET or DIET-based aspects of microbial physiology are growing. Mechanisms for have primarily been studied in defined cocultures which Geobacter species one the partners. Electrically conductive pili (e-pili) can be an important electrical conduit DIET. However, there may instances contacts made between transport proteins associated with outer membranes Alternatively, partners plug into carbon materials, such as granular activated carbon, cloth, biochar, long-range exchange without need e-pili. Magnetite promotes DIET, possibly by acting a substitute outer-surface c-type cytochromes. is primary mode some anaerobic digesters converting wastes to methane. Promoting materials shows promise stabilizing accelerating methane production digesters, permitting higher organic loading rates. Various lines evidence suggest terrestrial wetlands, source atmospheric also role oxidation coupled sulfate reduction, control releases. The finding serve electrons photosynthesis further broadens its potential environmental significance. Microorganisms capable good catalysts several bioelectrochemical technologies e-pili promising renewable electronic materials. study early stages, additional investigation required better understand diversity microorganisms importance flow environments, biochemistry

Language: Английский

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Electromicrobiology: the ecophysiology of phylogenetically diverse electroactive microorganisms

Nature Reviews Microbiology, Journal Year: 2021, Volume and Issue: 20(1), P. 5 - 19

Published: July 27, 2021

Language: Английский

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Advances towards understanding and engineering direct interspecies electron transfer in anaerobic digestion

Bioresource Technology, Journal Year: 2017, Volume and Issue: 244, P. 698 - 707

Published: Aug. 8, 2017

Language: Английский

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Hydrochar-Facilitated Anaerobic Digestion: Evidence for Direct Interspecies Electron Transfer Mediated through Surface Oxygen-Containing Functional Groups

Environmental Science & Technology, Journal Year: 2020, Volume and Issue: 54(9), P. 5755 - 5766

Published: April 7, 2020

Acceleration of the anaerobic digestion (AD) process is crucial to achieving energy-efficient recycling organic wastes. Hydrochar produced by hydrothermal liquefaction biomass, yet its application in AD rarely reported. The present study showed that sewage sludge-derived hydrochar (SH) enhanced methane production rate glucose 37%. SH increased from acetate but did not affect acidification and H2/CO2. hydrogenotrophic methanogenesis, which could be due direct interspecies electron transfer (DIET) converting H+, e–, CO2 methane. Trichococcus Methanosaeta were dominant with SH. Label-free proteomic analysis was involved DIET as reflected up-regulation proteins methanogenesis. Hydrochars derived corn straw (CH), Enteromorpha algae (EH), poplar wood (PH), well activated carbon (AC), also tested process. SH, CH, EH obviously rates, 39%, 15%, 20% higher than control experiment, respectively. It neither electrical conductivity nor total redox property hydrochars AC abundances surface oxygen-containing functional groups correlated rates.

Language: Английский

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Microbiological insights into anaerobic digestion for biogas, hydrogen or volatile fatty acids (VFAs): a review

Bioengineered, Journal Year: 2022, Volume and Issue: 13(3), P. 6521 - 6557

Published: Feb. 25, 2022

In the past decades, considerable attention has been directed toward anaerobic digestion (AD), which is an effective biological process for converting diverse organic wastes into biogas, volatile fatty acids (VFAs), biohydrogen, etc. The microbial bioprocessing takes part during AD of substantial significance, and one crucial approaches deep adequate understanding manipulating it different products microbiology. Due to highly complexity microbiome, critically important study involved microorganisms in AD. recent years, addition traditional methods, novel molecular techniques meta-omics have developed provide accurate details about communities Better microbiomes could guide us identifying controlling various factors both improving diverting metabolic pathway production selective bio-products. This review covers platforms that results final from microbiological point view. also highlights distinctive interactions occurring among communities. Furthermore, assessment these existing digesters discussed more insights their structure, dynamics, pathways. Moreover, affecting each platform are highlighted. Finally, provides some applications bio-products deals with challenges future perspectives

Language: Английский

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Why do DIETers like drinking: Metagenomic analysis for methane and energy metabolism during anaerobic digestion with ethanol

Water Research, Journal Year: 2019, Volume and Issue: 171, P. 115425 - 115425

Published: Dec. 23, 2019

Language: Английский

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Magnetite Triggering Enhanced Direct Interspecies Electron Transfer: A Scavenger for the Blockage of Electron Transfer in Anaerobic Digestion of High-Solids Sewage Sludge

Environmental Science & Technology, Journal Year: 2018, Volume and Issue: 52(12), P. 7160 - 7169

Published: May 21, 2018

At present, high-solids anaerobic digestion of sewage sludge has drawn great attention due to the superiority its small land area footprint and low energy consumption. However, a high organic loading rate may cause acids accumulation ammonia inhibition, thus leading an inhibited pseudo-steady state in which electron transfer through interspecies hydrogen (IHT) between acetogens methanogens is blocked. In this study, adding 50 mg/g TS (total solid) magnetite clearly reduced short-chain fatty accelerated methane production by 26.6%. As demonstrated, individual processes could not be improved when methanogenesis was interrupted. Analyzing stable carbon isotopes investigating pathways using acetate H2/CO2 as substrates together proved that direct (DIET) enhanced magnetite. Metatranscriptomic analysis determination key enzymes showed IHT partially substituted DIET, acetate-dependent after blockage scavenged. Additionally, expression both pili c-type cytochromes found decrease, indicating replace their roles for efficient methanogens; thus, robust chain established.

Language: Английский

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Geobacter Protein Nanowires

Frontiers in Microbiology, Journal Year: 2019, Volume and Issue: 10

Published: Sept. 24, 2019

The study of electrically conductive protein nanowires in Geobacter sulfurreducens has led to new concepts for long-range electron extracellular transport, as well the development sustainable materials and electronic devices with novel functions. Until recently, pili (e-pili), assembled from PilA pilin monomer, were only known nanowires. However, filaments comprised multi-heme c-type cytochrome, OmcS, are present some preparations G. outer-surface proteins. purpose this review is evaluate available evidence on vivo expression e-pili OmcS their biological function. Abundant literature demonstrates that expresses e-pili, which required transport Fe(III) oxides through biofilms. In contrast, there no definitive yet wild-type express long extending cells, deleting gene actually increases biofilm conductivity. does not support concern many previous studies mistakenly studying filaments. For example, heterologous aromatic-rich monomer metallireducens conductivity individual more than 5000-fold, whereas an aromatic-poor reduced 1000-fold. This million-fold range nanowire was achieved while maintaining 3 nm diameter characteristic e-pili. Purification methods eliminate all traces yield highly e-pilin microbes do produce or any other cytochromes. Future need be cognizant importance environmentally relevant growth conditions because artificial laboratory culture can rapidly select against expression. Principles derived have enabled identification non-cytochrome diverse bacteria archaea. A similar search cytochrome appendages warranted. Both offer design options synthesis protein-based 'green' electronics, may primary driving force these structures near future.

Language: Английский

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Microbial extracellular electron transfer and strategies for engineering electroactive microorganisms

Biotechnology Advances, Journal Year: 2020, Volume and Issue: 53, P. 107682 - 107682

Published: Dec. 15, 2020

Language: Английский

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The mechanisms of biochar interactions with microorganisms in soil

Environmental Geochemistry and Health, Journal Year: 2019, Volume and Issue: 42(8), P. 2495 - 2518

Published: Sept. 14, 2019

Language: Английский

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