Microbiota is structured by gut regions, life stage, and diet in the Black Soldier Fly (Hermetia illucens)

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: Aug. 17, 2023

The larvae of the Black Soldier Fly ( Hermetia illucens ) provide numerous ecological benefits, leading to significant commercial advancements. These benefits include bioconversion low-value waste into high-value feed and soil amendments. Understanding how bacterial eukaryotic microbiota communities affect host performance becomes vital for optimization specialization industrial-scale rearing. This study investigates H. -associated taxonomic composition dynamics across developmental cycle (eggs, neonates, larvae, prepupae, imago X0 second generation X1) when reared on two substrates: (i) plant-based (Housefly Gainesville diet) (ii) animal-based (poultry hatchery waste). By using 16S gene amplicon metataxonomic approach, we found that results revealed inherited from parents a different substrate may have induced dysbiosis in progeny. Specifically, interaction networks individuals showed high prevalence negative interactions low connectivity. Proteobacteria (39–92%), Firmicutes (4–39%), Bacteroidota (1–38%), Actinobacteria (1–33%). In animal feed-reared individuals, reached highest relative abundance (10–80%), followed by (6–55%), (1–31%), (0–22%). rearing was main driver composition, while stage influenced only whole individual's composition. Gut regions were associated with distinct richness, diversity decreasing along digestive tract. For first time, microeukaryotes other than Fungi investigated 18S genetic marker sequencing novel blocking primers specific Fly. Microeukaryotes are neglected part multitrophic can similar effects their hosts as microbiota. seven orders identified black soldier flies, including potential pathogens (e.g., Aplicomplexa group). Nucletmycea dominant class throughout development, Holozoa Stramenophiles. eukaryote structured stages but not gut regions. Insights this stepping stone toward microbiological flies industrial rearing, highlighting synthetic assembly should be tailored environment at targeted stage.

Language: Английский

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Biopreservation and the Safety of Fish and Fish Products, the Case of Lactic Acid Bacteria: A Basic Perspective

Fishes, Journal Year: 2024, Volume and Issue: 9(8), P. 303 - 303

Published: Aug. 2, 2024

Through fishing and aquaculture activities, humans have access to fish, which are a basic food source in the diet due their nutritional value. Fish widely distributed commercialized worldwide different products (e.g., whole fresh, filleted, sliced, frozen, dried, smoked, salted, canned among others). Because of composition value, fish highly susceptible spoilage contamination, mainly by microorganisms, compromising safety, shelf life, availability; therefore, consuming can become risk public health. Foodborne diseases considered important global health problems because incidence, consequences, mortality, negative economic impact on population. Among foods commonly associated with foodborne contaminated various agents that harmful throughout chain. constant growth population demand for greater quantities food, search development technologies generation availability safe sensorial qualities has increased. This is how biopreservation emerges, which, through application lactic acid bacteria and/or metabolites, positioned as sustainable, economic, simple alternative obtaining making them available human animal consumption. Therefore, this work focuses providing general perspective information search, collection, analysis databases, such Google Scholar, SciELO, Redalyc, ScienceDirect, institutional repositories, regarding production, properties, diseases, causal agents, associations products. Additionally, study describes process use metabolites extend life promote safety sensory intended

Language: Английский

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Investigating Both Mucosal Immunity and Microbiota in Response to Gut Enteritis in Yellowtail Kingfish

Microorganisms, Journal Year: 2020, Volume and Issue: 8(9), P. 1267 - 1267

Published: Aug. 20, 2020

The mucosal surfaces of fish play numerous roles including, but not limited to, protection against pathogens, nutrient digestion and absorption, excretion nitrogenous wastes osmotic regulation. During infection or disease, these act as the first line defense, where immune system interacts closely with associated microbiota to maintain homeostasis. This study evaluated microbial changes across gut skin in yellowtail kingfish displaying signs inflammation, well explored host gene expression tissues order improve our understanding underlying mechanisms that contribute emergence conditions. For this, we obtained analyzed 16S rDNA transcriptomic (RNA-Seq) sequence data from mucosa exhibiting different health states (i.e., healthy at early late stages enteritis). Both were perturbed by disease. More specifically, gastrointestinal diseased was dominated an uncultured Mycoplasmataceae sp., stage disease showed a significant loss diversity skin. Using transcriptomics, found only few genes significantly differentially expressed gut. In contrast, differed widely between states, particular These several metabolic pathways reflected weakened host. Altogether, this highlights sensitivity surface response inflammation.

Language: Английский

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Teleost skin microbiome: An intimate interplay between the environment and the host immunity

Fish & Shellfish Immunology, Journal Year: 2023, Volume and Issue: 139, P. 108869 - 108869

Published: June 5, 2023

Language: Английский

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Microbial communities associated with the skin, gill, and gut of large yellow croaker (Larimichthys crocea)

BMC Microbiology, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 11, 2025

The microbiota inhabiting the surface of fish mucosal tissue play important roles in nutrition, metabolism and immune system their host. However, most investigations on microbial symbionts have focused gut, but associated with external tissues (such as skin gill) is poorly understood. This study characterised traits dynamic communities skin, gill gut large yellow croaker (Larimichthys crocea) culturing net enclosures or pens at different sampling times (with seasonal transition). Results revealed structure function differed according to croaker. richness diversity were significantly higher than that gut. Discriminative taxa such Psychrobacter Enterobacterales gill, Fusobacterium discriminative predictive functions identified Furthermore, environmental-related factors time/season culture method) had impacts differently. composition changed over time, difference across was significant among three tissues. method impacted microbiota, no found between enclosure pen. These results indicated more diverse affected by other provides new insights into structure, environmental response pattern, relationship host health

Language: Английский

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