OpaR Exerts a Dynamic Control over c-di-GMP Homeostasis and cpsA Expression in Vibrio parahaemolyticus through Its Regulation of ScrC and the Trigger Phosphodiesterase TpdA

Microbiology Spectrum, Journal Year: 2023, Volume and Issue: 11(3)

Published: May 18, 2023

The second messenger cyclic dimeric GMP (c-di-GMP) plays a central role in controlling decision-making processes that are vitally important for the environmental survival of human pathogen Vibrio parahaemolyticus. mechanisms by which c-di-GMP levels and biofilm formation dynamically controlled V. parahaemolyticus poorly understood. Here, we report involvement OpaR metabolism its effects on expression trigger phosphodiesterase (PDE) TpdA biofilm-matrix related gene cpsA. Our results revealed negatively modulates tpdA maintaining baseline level c-di-GMP. OpaR-regulated PDEs ScrC, ScrG, VP0117 enable upregulation tpdA, to different degrees, absence OpaR. We also found dominant degradation under planktonic conditions compared other PDEs. In cells growing solid medium, observed degrader alternates between ScrC TpdA. contrasting cpsA media forming biofilms over glass. These suggest can act as double-edged sword control perhaps development response understood factors. Finally, using an in-silico analysis, indicate outlets regulatory module impact decision making during motile-to-sessile transition IMPORTANCE is extensively used bacterial crucial social adaptations such formation. explore quorum-sensing regulator OpaR, from parahaemolyticus, dynamic signaling production. homeostasis Lysogeny Broth agar alternate time. Furthermore, roles biofilm-related surfaces growth conditions. This dual has not been reported orthologues HapR cholerae. It investigate origins consequences differences closely distantly pathogens better understand pathogenic behavior evolution.

Language: Английский

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Molecular docking and dynamics simulation studies uncover the host-pathogen protein-protein interactions in Penaeus vannamei and Vibrio parahaemolyticus

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(1), P. e0297759 - e0297759

Published: Jan. 24, 2024

Shrimp aquaculture contributes significantly to global economic growth, and the whiteleg shrimp, Penaeus vannamei , is a leading species in this industry. However, Vibrio parahaemolyticus infection poses major challenge ensuring success of P . aquaculture. Despite its significance industry, biological knowledge pathogenesis remains unclear. Hence, study was conducted identify interaction sites binding affinity between several immune-related proteins with V associated virulence factors. Potential host pathogen were identified using molecular docking dynamics (MD) simulation. The vannamei-V protein-protein Complex 1 (Ferritin-HrpE/YscL family type III secretion apparatus protein), 2 (Protein kinase domain-containing protein-Chemotaxis CheY 3 (GPCR-Chemotaxis protein) found interact -4319.76, -5271.39, -4725.57 docked score formation intermolecular bonds at interacting residues. scores 1, 2, validated MD simulation analysis, which revealed these complexes greatly contribute interactions proteins, free energies -22.50 kJ/mol, -30.20 -26.27 respectively. This finding illustrates capability computational approaches search for pathogen, could increase spp. on shrimps, then can be used assist development effective treatment.

Language: Английский

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The LuxO-OpaR quorum-sensing cascade differentially controls Vibriophage VP882 lysis-lysogeny decision making in liquid and on surfaces

PLoS Genetics, Journal Year: 2024, Volume and Issue: 20(7), P. e1011243 - e1011243

Published: July 30, 2024

Quorum sensing (QS) is a process of cell-to-cell communication that bacteria use to synchronize collective behaviors. QS relies on the production, release, and group-wide detection extracellular signaling molecules called autoinducers. Vibrios two systems: LuxO-OpaR circuit VqmA-VqmR circuit. Both circuits control group behaviors including biofilm formation surface motility. The Vibrio parahaemolyticus temperate phage φVP882 encodes VqmA homolog (called VqmAφ). When VqmAφ produced by lysogens, it binds host-produced autoinducer DPO launches lytic cascade. This activity times induction lysis with high host cell density presumably promotes maximal transmission new cells. Here, we explore whether, in addition from lysogeny, controls initial establishment lysogeny naïve Using mutagenesis, infection assays, phenotypic analyses, show connects its lysis-lysogeny decision both whether resides liquid or surface. Host cells low-cell-density state primarily undergo lysogenic conversion. regulator LuxO~P conversion planktonic By contrast, ScrABC surface-sensing system regulates surface-associated abundance second messenger molecule cyclic diguanylate, which turn, modulates scrABC operon only expressed when repressor, OpaR, absent. Thus, at low density, QS-dependent derepression drives These results demonstrate integrates cues multiple sensory pathways into lifestyle making upon cell.

Language: Английский

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The two-component system expression patterns and immune regulatory mechanism of Vibrio parahaemolyticus with different genotypes at the early stage of infection in THP-1 cells

mSystems, Journal Year: 2023, Volume and Issue: unknown

Published: July 11, 2023

Vibrio parahaemolyticus must endure various challenging circumstances while being swallowed by phagocytes of the innate immune system. Moreover, bacteria should recognize and react to environmental signals quickly in host cells. Two-component system (TCS) is an important way for perceive external transmit them interior trigger associated regulatory mechanism. However, function V. TCS cells unclear. Here, expression patterns -infected THP-1 cell-derived macrophages at early stage were studied first time. Based on protein-protein interaction network analysis, we mined analyzed seven critical genes with excellent research value regulating macrophages, as shown below. VP1503, VP1502, VPA0021, VPA0182 could regulate ATP-binding-cassette (ABC) transport VP1735, uvrY, peuR might interact thermostable hemolysin proteins, DNA cleavage-related TonB-dependent siderophore enterobactin receptor, respectively, which may assist infected macrophages. Subsequently, potential escape pathways explored RNA-seq. The results showed that infect controlling apoptosis, actin cytoskeleton, cytokines. In addition, found (peuS/R) enhance toxicity contribute activation macrophage apoptosis. IMPORTANCE This study offer crucial new insights into pathogenicity without tdh trh genes. also provided a novel direction inquiry pathogenic mechanism suggested several key regulation interaction.

Language: Английский

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Evaluation of Therapeutic Efficiency of Stylicin against Vibrio parahaemolyticus Infection in Shrimp Penaeus vannamei through Comparative Proteomic Approach

Probiotics and Antimicrobial Proteins, Journal Year: 2022, Volume and Issue: 16(1), P. 76 - 92

Published: Dec. 2, 2022

Language: Английский

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