Metabolic engineering of fast-growing Vibrio natriegens for efficient pyruvate production DOI Creative Commons
Fengli Wu, Shucai Wang, Yanfeng Peng

et al.

Microbial Cell Factories, Journal Year: 2023, Volume and Issue: 22(1)

Published: Sept. 4, 2023

Pyruvate is a widely used value-added chemical which also serves as hub of various metabolic pathways. The fastest-growing bacterium Vibrio natriegens promising chassis for synthetic biology applications with high substrate uptake rates. aim this study was to investigate if the rates V. enable pyruvate production at productivities.Two prophage gene clusters and several essential genes biosynthesis byproducts were first deleted. In order promote accumulation, key aceE encoding dehydrogenase complex E1 component down-regulated reduce carbon flux into tricarboxylic acid cycle. Afterwards, expression ppc phosphoenolpyruvate carboxylase fine-tuned balance cell growth synthesis. resulting strain PYR32 able produce 54.22 g/L from glucose within 16 h, yield 1.17 mol/mol an average productivity 3.39 g/L/h. addition, efficiently convert sucrose or gluconate titers.A novel engineered capable provide higher in This lays foundation its derivatives fast-growing natriegens.

Language: Английский

Unraveling the Structure and Function of Melanin through Synthesis DOI
Wei Cao, Xuhao Zhou, Naneki C. McCallum

et al.

Journal of the American Chemical Society, Journal Year: 2021, Volume and Issue: 143(7), P. 2622 - 2637

Published: Feb. 9, 2021

Melanin is ubiquitous in living organisms across different biological kingdoms of life, making it an important, natural biomaterial. Its presence nature from microorganisms to higher animals and plants attributed the many functions melanin, including pigmentation, radical scavenging, radiation protection, thermal regulation. Generally, melanin classified into five types-eumelanin, pheomelanin, neuromelanin, allomelanin, pyomelanin-based on various chemical precursors used their biosynthesis. Despite its long history study, exact makeup remains unclear, moreover has inherent diversity complexity structure, likely properties that remain be identified. Synthetic mimics have begun play a broader role unraveling structure function relationships melanins. In past decade, polydopamine, which served as conventional form synthetic eumelanin, dominated literature melanin-based materials, while analogues other melanins received far less attention. this perspective, we will discuss synthesis materials with special focus beyond polydopamine. We emphasize efforts elucidate biosynthetic pathways structural characterization approaches can harnessed interrogate specific structure-function relationships, electron paramagnetic resonance (EPR) solid-state nuclear magnetic (ssNMR) spectroscopy. believe timely Perspective introduce class biopolymer chemistry community, where hope stimulate new opportunities novel, poly-functional materials.

Language: Английский

Citations

276
Microbial melanin: Recent advances in biosynthesis, extraction, characterization, and applications DOI

Sanju Singh,

Satish Balasaheb Nimse,

Doniya Elze Mathew

et al.

Biotechnology Advances, Journal Year: 2021, Volume and Issue: 53, P. 107773 - 107773

Published: May 20, 2021

Language: Английский

Citations

149
Microalgae-bacteria consortium for wastewater treatment and biomass production DOI
Lisa Aditya, T.M.I. Mahlia, Luong N. Nguyen

et al.

The Science of The Total Environment, Journal Year: 2022, Volume and Issue: 838, P. 155871 - 155871

Published: May 11, 2022

Language: Английский

Citations

136
Phycobiliproteins: Structural aspects, functional characteristics, and biotechnological perspectives DOI Creative Commons
Jorge Dagnino‐Leone,

Cristina Pinto Figueroa,

Mónica Latorre Castañeda

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2022, Volume and Issue: 20, P. 1506 - 1527

Published: Jan. 1, 2022

Phycobiliproteins (PBPs) are fluorescent proteins of various colors, including fuchsia, purple-blue and cyan, that allow the capture light energy in auxiliary photosynthetic complexes called phycobilisomes (PBS). PBPs have several highly preserved structural physicochemical characteristics. In PBS context, function is luminous 450–650 nm range delivers it to photosystems allowing photosynthesis take place. Besides harvesting function, also shown multiple biological activities, antioxidant, antibacterial antitumours, making them an interesting focus for different biotechnological applications areas like biomedicine, bioenergy scientific research. Nowadays, main sources cyanobacteria micro macro algae from phylum Rhodophyta. Due diverse activities PBPs, they attracted attention industries, such as food, biomedical cosmetics. This why a large number patents related production, extraction, purification their application cosmetics, biopharmaceuticals or diagnostic been generated, looking less ecological impact natural prairies macroalgae culture time higher productivity satisfy markets require high amounts these molecules. this review, we summarize characteristics biosynthesys applications. We address current trends future perspectives market.

Language: Английский

Citations

119
Self-pigmenting textiles grown from cellulose-producing bacteria with engineered tyrosinase expression DOI Creative Commons
Kenneth T. Walker, Ivy S. Li,

Jennifer Keane

et al.

Nature Biotechnology, Journal Year: 2024, Volume and Issue: unknown

Published: April 2, 2024

Abstract Environmental concerns are driving interest in postpetroleum synthetic textiles produced from microbial and fungal sources. Bacterial cellulose (BC) is a promising sustainable leather alternative, on account of its material properties, low infrastructure needs biodegradability. However, for alternative like BC to be fully sustainable, ways dye need developed alongside production methods. To address this, we genetically engineer Komagataeibacter rhaeticus create bacterial strain that grows self-pigmenting BC. Melanin biosynthesis the bacteria recombinant tyrosinase expression achieves dark black coloration robust use. Melanated can scaled up construction prototype fashion products, illustrate potential combining engineered self-pigmentation with tools biology, through optogenetic patterning gene cellulose-producing bacteria. With this study, demonstrate genetic engineering current future methods textile biofabrication has new class textiles.

Language: Английский

Citations

20
Vibrio natriegens: an ultrafast‐growing marine bacterium as emerging synthetic biology chassis DOI
Josef Hoff, Benjamin J. Daniel, Daniel Stukenberg

et al.

Environmental Microbiology, Journal Year: 2020, Volume and Issue: 22(10), P. 4394 - 4408

Published: June 15, 2020

Summary The marine bacterium Vibrio natriegens is the fastest‐growing non‐pathogenic known to date and gaining more attention as an alternative chassis organism Escherichia coli . A recent wave of synthetic biology efforts has focused on establishment molecular tools in this fascinating organism, now enabling exciting applications – from speeding up our everyday laboratory routines increasing pace biotechnological production cycles In review, we seek give a broad overview literature V. , spanning all way its initial isolation latest applications. We discuss natural ecological niche interactions with other organisms, unveil some extraordinary traits, review genomic organization insight into diverse metabolism key physiological insights required further develop chassis. By providing comprehensive established genetic tools, methods highlight current possibilities but also identify gaps that could drive future lines research, hopefully stimulating growth research community.

Language: Английский

Citations

105
Bio-synthesis of food additives and colorants-a growing trend in future food DOI
Lichao Sun, Fengjiao Xin, Hal S. Alper

et al.

Biotechnology Advances, Journal Year: 2020, Volume and Issue: 47, P. 107694 - 107694

Published: Dec. 31, 2020

Language: Английский

Citations

77
Vibrio natriegens genome‐scale modeling reveals insights into halophilic adaptations and resource allocation DOI Creative Commons

Lucas Coppens,

Tanya Tschirhart, Dagmar H. Leary

et al.

Molecular Systems Biology, Journal Year: 2023, Volume and Issue: 19(4)

Published: Feb. 27, 2023

Abstract Vibrio natriegens is a Gram‐negative bacterium with an exceptional growth rate that has the potential to become standard biotechnological host for laboratory and industrial bioproduction. Despite this burgeoning interest, current lack of organism‐specific qualitative quantitative computational tools hampered community's ability rationally engineer bacterium. In study, we present first genome‐scale metabolic model (GSMM) V. . The GSMM (iLC858) was developed using automated draft assembly extensive manual curation validated by comparing predicted yields, central fluxes, viable carbon substrates, essential genes empirical data. Mass spectrometry‐based proteomics data confirmed translation at least 76% enzyme‐encoding be expressed during aerobic in minimal medium. iLC858 subsequently used carry out comparison between organism Escherichia coli , leading analysis architecture ' respiratory ATP‐generating system discovery role sodium‐dependent oxaloacetate decarboxylase pump. were further investigate additional halophilic adaptations Finally, utilized create Resource Balance Analysis study allocation resources. Taken together, models presented provide useful guide engineering efforts

Language: Английский

Citations

24
The Amazing World of Biological Pigments: A Review on Microbial Melanins DOI
Divya Singh,

Deepshikha Deepshikha,

Venkatesh Chaturvedi

et al.

Dyes and Pigments, Journal Year: 2025, Volume and Issue: unknown, P. 112711 - 112711

Published: Feb. 1, 2025

Language: Английский

Citations

1
An Overview on Industrial and Medical Applications of Bio-Pigments Synthesized by Marine Bacteria DOI Creative Commons
Ali Nawaz, Rida Chaudhary, Zinnia Shah

et al.

Microorganisms, Journal Year: 2020, Volume and Issue: 9(1), P. 11 - 11

Published: Dec. 22, 2020

Marine bacterial species contribute to a significant part of the oceanic population, which substantially produces biologically effectual moieties having various medical and industrial applications. The use marine-derived pigments displays snowballing effect in recent times, being natural, environmentally safe, health beneficial compounds. Although isolating marine bacteria is strenuous task, these are still compelling subject for researchers, due their promising avenues numerous Marine-derived serve as valuable products food, pharmaceutical, textile, cosmetic industries attributes, including anticancer, antimicrobial, antioxidant, cytotoxic activities. Biodegradability higher environmental compatibility further strengthen bio-pigments over artificially acquired colored molecules. Besides that, hazardous effects associated with consumption synthetic colors substantiated dyes color additives well. This review sheds light on sources pigmented compounds along applicability therapeutic insights based data available literature. It also encompasses need introducing global pigment industry, highlighting future potential, aiming worldwide economy.

Language: Английский

Citations

56