Isolation and Identification of Vibrio Species from Different Types of Water Sources Along with Their Drug Susceptible Pattern

Biomedical and Biotechnology Research Journal (BBRJ), Journal Year: 2024, Volume and Issue: 8(2), P. 207 - 212

Published: April 1, 2024

Abstract Background: Human activities are almost usually the cause of contaminated water supplies and places with large population densities intensive land usage particularly prone to pollution drinking sources. It is challenging detoxify polluted Cholera an acute form diarrheal disease that plagued human civilization over centuries. Vibrio spp. , commonly found in aquatic environments, most common bacterial gastroenteritis world, those may spread humans through ingestion or exposure seawater. Methods: In present study, a total 12 samples were collected from four types sources for isolation along their drug-resistant pattern conventional, biochemical, antibiotic disc diffusion methods . Results: The enriched into alkaline peptone then inoculated culture media such as nutrient agar, MacConkey, TCBs agar medium. After incubation 24 h at 37°C on TCBS yellow green colonies screened out biochemical identification. Nonsucrose-fermenting parahaemolyticus mimicus vulnificus showed colonies, sucrose-fermenting cholera alginolyticus TCBS. antibiotics ERY (15 μg), PCN (10 CEX (30 VAN μg) completely resistant while more effective against SXT (25 CHL GEN μg). However, ciprofloxacin nitrofurantoin moderate efficacy. Conclusion: To expand current knowledge occurrence, ecological niche, persistence potential pathogenic extensive laboratory experiment required. sum up, this kind information crucial bringing attention existence enabling prompt implementation preventive measures.

Language: Английский

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Prevalence and antimicrobial susceptibility pattern of Vibrio cholerae isolates from cholera outbreak sites in Ethiopia

BMC Public Health, Journal Year: 2024, Volume and Issue: 24(1)

Published: July 31, 2024

Cholera is an acute infectious disease caused by ingestion of contaminated food or water with Vibrio cholerae. remains a global threat to public health and indicator inequity lack social development. The aim this study was assess the prevalence antimicrobial susceptibility pattern V. cholerae from cholera outbreak sites in Ethiopia. Across-sectional conducted May 2022 October 2023 across different regions Ethiopia: Oromia National Regional State, Amhara State Addis Ababa City Administration. A total 415 fecal samples were collected three regions. Two milliliter each participants. cultured on Blood Agar, MacConkey Agar Thiosulfate Citrate Bile Salt Sucrose Agar. series biochemical tests Oxidase test, String Motility, Indole, Citrate, Gas production, H2S Urease test used identify species. Both polyvalent monovalent antisera for agglutination differentiate serogroup serotypes. In addition, Kirby-Bauer Disk diffusion antibiotic method done. Data registered epi-enfo version 7 analyzed Statistical Package Social Science 25. Descriptive statistics determine Logistic regression model fitted p-value < 0.05 considered as statically significant. 30.1%. Majority isolates 43.2% (n = 54) followed 31.2% 39) Administration 25.6% 32). Most O1 serogroups 90.4% 113) Ogawa serotypes 86.4% 108). susceptible ciprofloxacin 100% 125), tetracycline 72% 90) gentamycin 68% 85). More than half resistant trimethoprim-sulfamethoxazole 62.4% 78) ampicillin 56.8% 71). study, participants unable read write about four times more at risk infection (AOR: 3.8, 95% CI: 1.07–13.33). consumption river 2.8, 1.08–7.08). our revealed high samples. predominant Ogawa, respectively. Fortunately, showed most tested antibiotics. Drinking identified associated factor infection. Protecting community drinking provision safe treated could reduce outbreaks areas.

Language: Английский

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Towards a Comprehensive Definition of Pandemics and Strategies for Prevention: A Historical Review and Future Perspectives

Microorganisms, Journal Year: 2024, Volume and Issue: 12(9), P. 1802 - 1802

Published: Aug. 30, 2024

The lack of a universally accepted definition pandemic hinders comprehensive understanding and effective response to these global health crises. Current definitions often quantitative criteria, rendering them vague limiting their utility. Here, we propose refined that considers the likelihood susceptible individuals contracting an infectious disease culminates in widespread transmission, increased morbidity mortality, profound societal, economic, political consequences. Applying this retrospectively, identify 22 pandemics occurred between 165 2024 AD were caused by variety diseases, including smallpox (Antonine American), plague (Justinian, Black Death, Third Plague), cholera (seven pandemics), influenza (two Russian, Spanish, Asian, Hong Kong, swine), AIDS, coronaviruses (SARS, MERS, COVID-19). This work presents analysis past both emerging re-emerging pathogens, along with epidemiological characteristics, societal impact, evolution public responses. We also highlight need for proactive measures reduce risk future pandemics. These strategies include prioritizing surveillance zoonotic conserving biodiversity counter wildlife trafficking, minimizing potential spillover events. In addition, interventions such as promoting alternative protein sources, enforcing closure live animal markets biodiversity-rich regions, fostering collaboration among diverse stakeholders are critical preventing Crucially, improving systems will require concerted efforts local, national international entities, laboratories, field researchers, conservationists, government agencies other stakeholders. By collaborative networks establishing robust biorepositories, can strengthen our collective capacity detect, monitor, mitigate emergence transmission pathogens.

Language: Английский

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Vibrio cholerae virulence is blocked by chitosan oligosaccharide-mediated inhibition of ChsR activity

Nature Microbiology, Journal Year: 2024, Volume and Issue: 9(11), P. 2909 - 2922

Published: Oct. 16, 2024

Language: Английский

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Spatio-temporal patterns of cholera outbreak in rural settings of Ethiopia, 2023

Heliyon, Journal Year: 2025, Volume and Issue: 11(2), P. e41962 - e41962

Published: Jan. 1, 2025

The aim of this study was to assess the spatio-temporal pattern cholera in rural settings Ethiopia. A spatiotemporal analysis daily cases 59 Kebeles across 7 districts Gedeo zone from April 2 November 18, 2023, obtained Zone Health Department, conducted. global Moran's I statistic used for spatial autocorrelation analysis, and retrospective space-time scan analyze clusters cholera. Throughout outbreak, 792 were reported, corresponding an annual incidence 169.4 per 100,000 population. distribution showed strong autocorrelation, with a coefficient 0.272 (P-value <0.001). Five statistically significant identified by statistics using discrete Poisson model. These overlapped time had longer durations relatively high risk areas. identification high-risk specific forms basis rapid public health emergency response resource allocation prioritizing significantly control eventually eliminate There is room improve outbreaks settings.

Language: Английский

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