Genomic and phylogenetic analysis of hypervirulent Klebsiella pneumoniae ST23 in Ireland

Microbial Genomics, Journal Year: 2025, Volume and Issue: 11(3)

Published: March 19, 2025

Hypervirulent Klebsiella pneumoniae (hv Kp ) has emerged as a pathogen of global concern associated with invasive community-acquired infections. The combination hypervirulence and carbapenem resistance can result in severe difficult-to-treat This retrospective study aimed to investigate the spread hv sequence type 23 (ST23) Ireland convergence hypervirulent (hv) antimicrobial genotypes. Short-read sequences (PE300) for 90 K. ST23 isolates were generated by Galway Reference Laboratory Services (GRLS). Isolates from screening swabs ( n =59), infections =18), non-invasive sites =12) hospital environment =1). virulence content assessed genomically using Kleborate (v2.2.0), ABRicate (v1.0.1) Platon (v1.6). vivo was murine model. All genotypically 88/90 having maximal score 5 including carriage key genes. Eighty-two per cent (74/90) carried carbapenemase gene bla OXA-48 / OXA-181 NDM-1 ), 42% genes 3 or more classes. Core genomic delineation revealed be clonal similar profiles. Two distinct clusters Irish detected consisting 82/90 isolates. infection demonstrated virulence. An established clone is circulating within causing both colonization patients. lack reliable methods makes its detection control healthcare setting challenging.

Language: Английский

Profiling the gut microbiota to assess infection risk in Klebsiella pneumoniae -colonized patients

Gut Microbes, Journal Year: 2025, Volume and Issue: 17(1)

Published: Feb. 18, 2025

Vornhagen et al. introduced a model combining gut microbiota structure and Klebsiella pneumoniae genotype to assess infection risk in K. pneumoniae-colonized patients. Building on their findings, we investigated the composition 16 colonized patients, five of whom had bloodstream infections at time fecal sampling. Importantly, did not apply original machine learning due small sample size our cohort. Instead, explored distribution key antimicrobial resistance stress genes analyzed community based amplicon sequence variants (ASVs) V3–V4 16S rRNA region. Notably, distinct gene profiles were observed both infected non-infected three patients without showed no detectable ASVs despite microbiological confirmation colonization. These findings highlight need integrate data into assessment address limitations taxonomic resolution size. Future studies should aim develop streamlined tools for clinical application

Language: Английский

Refining the gut colonization Zophobas morio larvae model using an oral administration of multidrug-resistant Escherichia coli

Journal of Global Antimicrobial Resistance, Journal Year: 2024, Volume and Issue: 39, P. 240 - 246

Published: Nov. 2, 2024

The darkling beetle Zophobas morio can be implemented as an alternative in vivo model to study different intestinal colonization aspects. Recently, we showed that its larvae colonized by multidrug-resistant Escherichia coli strains administered via contaminated food (for 7 days) for a total experimental duration of 28 days.

Language: Английский